KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant and Antimicrobial Activities of Combined Extracts of Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis

오배자, 우슬, 가자 및 감초를 포함하는 혼합추출물의 항산화 및 항균활성

  • 조홍석 (월드코스텍 기업부설연구소) ;
  • 강세원 (월드코스텍 기업부설연구소) ;
  • 김주희 (월드코스텍 기업부설연구소) ;
  • 최민주 (월드코스텍 기업부설연구소) ;
  • 유혜원 (월드코스텍 기업부설연구소) ;
  • 박으뜸 (조선대학교 생명공학과) ;
  • 전홍성 (조선대학교 생명공학과)
  • Received : 2014.01.03
  • Accepted : 2014.01.22
  • Published : 2014.02.27
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Abstract

In this study, antioxidant and antimicrobial activities of ethanolic crude extract and its five different solvent subfractions (namely, ethyl acetate fraction, n-butanol fraction, chloroform fraction, n-hexane fraction and the aqueous fraction) from a mixture of four different medicinal herbs (Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis) were investigated. Among all the tested mixture combination of four medicinal herbs, 5:3:1:1 ratio of Galla:Achyranthes : Terminalia : Glycyrrhiza had the best antimicrobial effects against four strains of microorganisms (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli) and exhibited the highest DPPH radical-scavenging activity. Further sub-fractions with solvents were screened for antioxidant and antimicrobial activities. Antioxidant activity in order was ethyl acetate fraction > n-butanol fraction > chloroform fraction > nhexane fraction > aqueous fraction. The n-butanol extracted fraction showed the highest level of antimicrobial activity in com- parison to other fractions. In addition, all those fractions did not show any cytotoxicity against human skin cell CCD-986sk. These results suggest that 5:3:1:1 combination extracts of medicinal herbs (Galla : Achyranthes : Terminalia : Glycyrrhiza) may be potentially used as a safe natural antimicrobial preservative.

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References

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