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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Bioactivity Analysis of Salidroside from Acer tegmentosum using On-line Screening HPLC-ABTS+ Assay

On-line Screening HPLC-ABTS+ Assay을 이용한 산청목으로부터 Salidroside의 분리 및 생물활성 분석

  • Lee, Kwang Jin (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Song, Na-Young (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group) ;
  • Ma, Jin Yeul (Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group)
  • 이광진 (한국한의학연구원 한의신약개발그룹) ;
  • 송나영 (한국한의학연구원 한의신약개발그룹) ;
  • 마진열 (한국한의학연구원 한의신약개발그룹)
  • Received : 2014.03.17
  • Accepted : 2014.04.25
  • Published : 2014.04.30
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Abstract

Acer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as on-line screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase fromAcer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as online screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase from 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum. 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum.

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References

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