Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effect of Culture Conditions on Characteristics of Growth and Production of Docosahexaenoic acid (DHA) by Schizochytrium mangrovei

배양조건에 따른 Schizochytrium mangrovei의 성장 및 Docosahexaenoic acid의 생산특성

  • Jeong, U-Cheol (Department of Marine Biology and Aquaculture/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Byeong-Dae (Department of Seafood Science and Technology, Gyeongsang National University) ;
  • Kang, Seok-Joong (Department of Marine Biology and Aquaculture/Institute of Marine Industry, Gyeongsang National University)
  • 정우철 (경상대학교 해양생명과학과/해양산업연구소) ;
  • 최병대 (경상대학교 해양식품공학과) ;
  • 강석중 (경상대학교 해양생명과학과/해양산업연구소)
  • Received : 2013.02.18
  • Accepted : 2013.11.25
  • Published : 2014.04.30
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Abstract

Both docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. Lipids in microbial cells play various biological roles and, consequently, much research has been carried out on their role in cell physiology. The lipid composition of microorganisms can exhibit considerable variations depending on environment. The effects of culture conditions, temperature (15, 20, 24, 28, 32 and $36^{\circ}C$), salinity (10, 20, 30, 40 and 50 psu), pH (pH5, 6, 7, 8 and 9), rotation speeds (50, 100, 150 and 200 rpm), carbon sources, nitrogen sources and C/N ratio on the production of docosahexaenoic acid, fatty-acid profiles, and acids secreted to the broth culture by the oleaginous microorganism, Schizochytrium mangrovei (KCTC 11117BP), were studied. Temperature (initially $28^{\circ}C$), salinity (20 psu), pH (pH7), rotation speeds (100 rpm), organism fatty acids, and secreted acids in the broth were varied during cultivation of S. mangrovei. At pH 7.0, S. mangrovei was able to accumulate lipids up to 40% of its biomass, with 13% (w/w) DHA content. The monosaccharides glucose and fructose, and yeast extract were suitable carbon and nitrogen sources, respectively. The primary omega-3 polyunsaturated fatty acid produced was docosahexaenoic acid.

Keywords

References

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