한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제22권2호
  • /
  • Pages.105-112
  • /
  • 2014
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지
DOI QR코드

DOI QR Code

머루전초, 독활전초, 감초 혼합추출물의 Amyloid β Protein (25-35) 유발 신경 독성에 대한 억제효과

Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity

  • 장지연 (충북대학교 수의과대학) ;
  • 성연희 (충북대학교 수의과대학)
  • Jang, Ji Yeon (College of Veterinary Medicine, Chungbuk National University) ;
  • Seong, Yeon Hee (College of Veterinary Medicine, Chungbuk National University)
  • 투고 : 2014.01.02
  • 심사 : 2014.02.17
  • 발행 : 2014.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study investigated an ethanol extract (SSB) of a mixture of three medicinal plants of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix for possible neuroprotective effects on neurotoxicity induced by Amyloid ${\beta}$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $15{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-30{\mu}g/m{\ell}$, SSB inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in cultured cortical neurons. Memory impairment and increase of acetylcholinesterase activity induced by intracerebroventricular injection of mice with 16 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with SSB (25, 50 and 100 mg/kg, p.o., for 8 days). From these results, it is suggested that antidementia effect of SSB is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that SSB may have a therapeutic role in preventing the progression of Alzheimer's disease.

키워드

참고문헌

  1. Alkam T, Nitta A, Mizoguchi H, Itoh A and Nabeshima T. (2007). A natural scavenger of peroxynitrites, rosmarinic acid, protects against impairment of memory induced by Abeta(25-35). Behavioural Brain Research. 180:139-145. https://doi.org/10.1016/j.bbr.2007.03.001
  2. Atack JR, Perry EK, Bonham JR, Perry RH, Tomlinson BE, Blessed G and Fairbairn A. (1983). Molecular forms of acetylcholinesterase in senile dementia of Alzheimer type: Selective loss of the intermediate(10S) form. Neuroscience Letters. 40:199-204. https://doi.org/10.1016/0304-3940(83)90302-6
  3. Bae K. (2000). The medicinal plants of Korea. Kyo-Hak Publishing Co. Seoul, Korea. p.362-363.
  4. Ban JY, Jeon SY, Bae K, Song KS and Seong YH. (2006). Catechin and epicatechin from Smilacis chinae rhizome protect cultured rat cortical neurons against amyloid beta protein(25-35)-induced neurotoxicity through inhibition of cytosolic calcium elevation. Life Sciences. 79:2251-2259. https://doi.org/10.1016/j.lfs.2006.07.021
  5. Butterfield DA, Drake J, Pocernich C and Castegna A. (2001). Evidence of oxidative damage in Alzheimer's disease brain: Central role for amyloid beta-peptide. Trends in Molecular Medicine. 7:548-554. https://doi.org/10.1016/S1471-4914(01)02173-6
  6. Chen CY, Jang JH, Park MH, Hwang SJ, Surh YJ and Park OJ. (2007). Attenuation of abeta-induced apoptosis of plant extract(Saengshik) mediated by the inhibition of mitochondrial dysfunction and antioxidative effect. Annals of the New York Academy of Sciences. 1095:399-411. https://doi.org/10.1196/annals.1397.043
  7. Cho SO, Ban JY, Kim JY, Jeong HY, Lee IS, Song KS, Bae K and Seong YH. (2009). Aralia cordata protects against amyloid beta protein(25-35)-induced neurotoxicity in cultured neurons and has antidementia activities in mice. Journal of Pharmacological Sciences. 111:22-32. https://doi.org/10.1254/jphs.08271FP
  8. Choung MG, Hwang YS, Kim GP, Ahn KG, Shim HS, Hong SB, Choi JH, Yu CY, Chung IM, Kim SH and Lim JD. (2013). Antimelanogenic effect and whitening of anthocyanin rich fraction from seeds of Liriope platyphylla. Korean Journal of Medicinal Crop Sciences. 21:361-371. https://doi.org/10.7783/KJMCS.2013.21.5.361
  9. Dang NH, Zhang X, Zheng M, Son KH, Chang HW, Kim HP, Bae K and Kang SS. (2005). Inhibitory constituents against cyclooxygenases from Aralia cordata Thunb. Archives of Pharmacal Research. 28:28-33. https://doi.org/10.1007/BF02975131
  10. Du Y and Ko KM. (2006). Oleanolic acid protects against myocardial ischemia-reperfusion injury by enhancing mitochondrial antioxidant mechanism mediated by glutathione and alpha-tocopherol in rats. Planta Medica. 72:222-227. https://doi.org/10.1055/s-2005-916197
  11. Ekinci FJ, Linsley MD and Shea TB. (2000). Beta-amyloid-induced calcium influx induces apoptosis in culture by oxidative stress rather than tau phosphorylation. Brain Research. 76:389-395. https://doi.org/10.1016/S0169-328X(00)00025-5
  12. Gasparini L, Ongini E and Wenk G. (2004). Non-steroidal anti-inflammatory drugs(NSAIDs) in Alzheimer's disease: Old and new mechanisms of action. Journal of Neurochemistry. 91:521-536. https://doi.org/10.1111/j.1471-4159.2004.02743.x
  13. Gitter BD, Cox LM, Rydel RE and May PC. (1995). Amyloid beta peptide potentiates cytokine secretion by interleukin-1 beta-activated human astrocytoma cells. Proceedings of the National Academy of Sciences of the United States of America. 92:10738-10741. https://doi.org/10.1073/pnas.92.23.10738
  14. Gray CW and Patel AJ. (1995). Neurodegeneration mediated by glutamate and beta-amyloid peptide: A comparison and possible interaction. Brain Research. 691:169-179. https://doi.org/10.1016/0006-8993(95)00669-H
  15. Ha DT, Chen QC, Hung TM, Youn UJ, Ngoc TM, Thuong PT, Kim HJ, Seong YH, Min BS and Bae K. (2009a). stillbenes and oligostilbenes from leaf and stem of Vitis amurensis and their cytotoxic activity. Archives of Pharmacal Research. 32:177-183. https://doi.org/10.1007/s12272-009-1132-2
  16. Ha DT, Kim H, Thuong PT, Ngoc TM, Lee I, Hung ND and Bae K. (2009b). Antioxidant and lipoxygenase inhibitory activity of oligostilbenes from the leaf and stem of Vitis amurensis. Journal of Ethnopharmacology. 125:304-309. https://doi.org/10.1016/j.jep.2009.06.019
  17. Han B, Woo E, Park M and Han Y. (1985). Studies on the antiinflammatory activity of Aralia continentalis (III). Archives of Pharmacal Research. 8:59-65. https://doi.org/10.1007/BF02912223
  18. Huang KC. (1999). The pharmacology of Chinese herbs. CRC press LLC, Boca Raton, Florida, USA. p.364-368
  19. Ivins KJ, Ivins JK, Sharp JP and Cotman CW. (1999). Multiple pathways of apoptosis in PC12 cells. CrmA inhibits apoptosis induced by beta-amyloid. The Journal of Biological Chemistry. 274:2107-2112. https://doi.org/10.1074/jbc.274.4.2107
  20. Jeong HY, Kim JY, Lee HK, Ha do T, Song KS, Bae K and Seong YH. (2010). Leaf and stem of Vitis amurensis and its active components protect against amyloid beta protein(25-35)-induced neurotoxicity. Archives of Pharmacal Research. 33:1655-1664. https://doi.org/10.1007/s12272-010-1015-6
  21. Jung MH, Song KS and Seong YH. (2012). Inhibitory effect of Chaenomeles sinensis fruit on amyloid ${\beta}$ protein(25-35)-induced neurotoxicity in cultured neurons and memory impairment in mice. Korean Journal of Medicinal Crop Sciences. 20:8-15. https://doi.org/10.7783/KJMCS.2012.20.1.008
  22. Kim JY, Kweon K, Lee HK, Kim SH, Yoo JD, Bae KH and Seong YH. (2011). Protective effect of an ethanol extract mixture of Curcuma longae radix, Phellinus linteus, and Scutellariae radix on oxidative neuronal damage. Korean Journal of Medicinal Crop Sciences. 19:31-37. https://doi.org/10.7783/KJMCS.2011.19.1.031
  23. Koh JY, Yang LL and Cotman CW. (1990). Beta-amyloid protein increases the vulnerability of cultured cortical neurons to excitotoxic damage. Brain Research. 533:315-320. https://doi.org/10.1016/0006-8993(90)91355-K
  24. Kontush A. (2001). Amyloid-beta: an antioxidant that becomes a pro-oxidant and critically contributes to Alzheimer's disease. Free radical Biology & Medicine. 31:1120-1131. https://doi.org/10.1016/S0891-5849(01)00688-8
  25. Kumar S, Sharma A, Madan B, Singhal V and Ghosh B. (2007). Isoliquiritigenin inhibits IkappaB kinase activity and ROS generation to block TNF-alpha induced expression of cell adhesion molecules on human endothelial cells. Biochemical Pharmacology. 73:1602-1612. https://doi.org/10.1016/j.bcp.2007.01.015
  26. Lee BY, Ban JY and Seong YH. (2005). Chronic stimulation of GABAA receptor with muscimol reduces amyloid beta protein(25-35)-induced neurotoxicity in cultured rat cortical cells. Neuroscience Research. 52:347-356. https://doi.org/10.1016/j.neures.2005.04.008
  27. Lee HK, Yang EJ, Kim JY, Song KS and Seong YH. (2012). Inhibitory effects of Glycyrrhizae radix and its active component, isoliquiritigenin, on Abeta(25-35)-induced neurotoxicity in cultured rat cortical neurons. Archives of Pharmacal Research. 35:897-904. https://doi.org/10.1007/s12272-012-0515-y
  28. Lee IS, Jin W, Zhang X, Hung TM, Song KS, Seong YH and Bae K. (2006). Cytotoxic and COX-2 inhibitory constituents from the aerial parts of Aralia cordata. Archives of Pharmacal Research. 29:548-555. https://doi.org/10.1007/BF02969263
  29. Maurice T, Lockhart BP and Privat A. (1996). Amnesia induced in mice by centrally administered beta-amyloid peptides involves cholinergic dysfunction. Brain Research. 706:181-193. https://doi.org/10.1016/0006-8993(95)01032-7
  30. Miranda S, Opazo C, Larrondo LF, Munoz FJ, Ruiz F, Leighton F and Inestrosa NC. (2000). The role of oxidative stress in the toxicity induced by amyloid beta-peptide in Alzheimer's disease. Progress in Neurobiology. 62:633-648. https://doi.org/10.1016/S0301-0082(00)00015-0
  31. Morais Cardoso S, Swerdlow RH and Oliveira CR. (2002). Induction of cytochrome c-mediated apoptosis by amyloid beta 25-35 requires functional mitochondria. Brain Research. 931:117-125. https://doi.org/10.1016/S0006-8993(02)02256-4
  32. Park HJ, Hong MS, Lee JS, Leem KH, Kim CJ, Kim JW and Lim S. (2005). Effects of Aralia continentalis on hyperalgesia with peripheral inflammation. Phytotherapy Research. 19:511-513. https://doi.org/10.1002/ptr.1693
  33. Pike CJ, Walencewicz-Wasserman AJ, Kosmoski J, Cribbs DH, Glabe CG and Cotman CW. (1995). Structure-activity analyses of beta-amyloid peptides: Contributions of the beta 25-35 region to aggregation and neurotoxicity. Journal of Neurochemistry. 64:253-265.
  34. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM and Pridham JB. (1995). The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research. 22:375-383. https://doi.org/10.3109/10715769509145649
  35. Sano M, Ernesto C, Thomas RG, Klauber MR, Schafer K, Grundman M, Woodbury P, Growdon J, Cotman CW, Pfeiffer E, Schneider LS and Thal LJ. (1997). A controlled trial of selegiline, alpha-tocopherol, or both as treatment for Alzheimer's disease. The Alzheimer's Disease Cooperative Study. The New England Journal of Medicine. 336:1216-1222. https://doi.org/10.1056/NEJM199704243361704
  36. Smith MA, Richey Harris PL, Sayre LM, Beckman JS and Perry G. (1997). Widespread peroxynitrite-mediated damage in Alzheimer's disease. The Journal of Neuroscience. 17:2653-2657. https://doi.org/10.1523/JNEUROSCI.17-08-02653.1997
  37. Stepanichev MY, Moiseeva YV, Lazareva NA, Onufriev MV and Gulyaeva NV. (2003). Single intracerebroventricular administration of amyloid-beta(25-35) peptide induces impairment in short-term rather than long-term memory in rats. Brain Research Bulletin. 61:197-205. https://doi.org/10.1016/S0361-9230(03)00118-7
  38. Tang SY, Whiteman M, Peng ZF, Jenner A, Yong EL and Halliwell B. (2004). Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional chinese medicines. Free radical Biology & Medicine. 36:1575-1587. https://doi.org/10.1016/j.freeradbiomed.2004.03.017
  39. Tohda C, Matsumoto N, Zou K, Meselhy MR and Komatsu K. (2004). Abeta(25-35)-induced memory impairment, axonal atrophy, and synaptic loss are ameliorated by M1, A metabolite of protopanaxadiol-type saponins. Neuropsychopharmacology. 29:860-868. https://doi.org/10.1038/sj.npp.1300388
  40. Ueda K, Shinohara S, Yagami T, Asakura K and Kawasaki K. (1997). Amyloid beta protein potentiates $Ca_2^+$ influx through L-type voltage-sensitive $Ca_2^+$ channels: A possible involvement of free radicals. Journal of Neurochemistry. 68:265-271.
  41. Um MY, Choi WH, Aan JY, Kim SR and Ha TY. (2006). Protective effect of Polygonum multiflorum Thunb on amyloid beta-peptide 25-35 induced cognitive deficits in mice. Journal of Ethnopharmacology. 104:144-148. https://doi.org/10.1016/j.jep.2005.08.054
  42. Wang Q, Yu S, Simonyi A, Rottinghaus G, Sun GY and Sun AY. (2004). Resveratrol protects against neurotoxicity induced by kainic acid. Neurochemical Research. 29:2105-2112. https://doi.org/10.1007/s11064-004-6883-z
  43. Yan XZ, Qiao JT, Dou Y and Qiao ZD. (1999). Beta-amyloid peptide fragment 31-35 induces apoptosis in cultured cortical neurons. Neuroscience. 92:177-184. https://doi.org/10.1016/S0306-4522(98)00727-1

피인용 문헌

  1. Antioxidant and Anticancer Properties of the Extracts from Lepisorus thunbergianus (Kaulf.) Ching vol.23, pp.4, 2015, https://doi.org/10.7783/KJMCS.2015.23.4.324