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Screening of Plant Extracts for Nemastatic Activity

선충 억제 활성 식물 추출물의 선발

  • Received : 2014.02.11
  • Accepted : 2014.03.20
  • Published : 2014.03.31

Abstract

A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of $5,000{\mu}g/ml$ were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.

식물 기원의 살선충 물질 탐색 연구의 일환으로, 178과, 1,677종 식물의 부위별 조추출물 2,714종을 이용하여 이들이 선충의 활성에 미치는 영향을 검토하였다. 선충억제 활성 검정은 96-well microplate를 이용하였으며 부식성선충 20마리를 조추출물 $5,000{\mu}g/ml$에 침지하고 7일간 매일 관찰하였다. 시험결과 2,362종(87.0%)의 조추출물은 부식성선충에 전혀 영향을 미치지 않았고, 187종(6.9%)의 조추출물은 부식성선충의 활성을 50% 정도 억제하였고, 95종(3.5%)의 조추출물은 90% 이상 억제를 보였으며, 70종(2.6%)의 조추출물에서는 오히려 부식성선충이 번식되었다. 1차 검정에서 50% 이상 선충억제 활성을 보인 추출물중에서, 25개의 추출물을 임의로 선택하여 2, 3차(부식성선충), 4차(고구마뿌리혹선충 유충) 반복 시험을 수행한 결과, 약 44%의 시료는 지속적인 선충억제 활성을 보였다. 이번 시험에서 선충억제 활성을 나타낸 282개의 식물 조추출물은 앞으로 식물 기원의 살선충 물질 연구시 참고 자료로 활용될 수 있을 것이다.

Keywords

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