식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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선충 억제 활성 식물 추출물의 선발

Screening of Plant Extracts for Nemastatic Activity

  • 투고 : 2014.02.11
  • 심사 : 2014.03.20
  • 발행 : 2014.03.31
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초록

식물 기원의 살선충 물질 탐색 연구의 일환으로, 178과, 1,677종 식물의 부위별 조추출물 2,714종을 이용하여 이들이 선충의 활성에 미치는 영향을 검토하였다. 선충억제 활성 검정은 96-well microplate를 이용하였으며 부식성선충 20마리를 조추출물 $5,000{\mu}g/ml$에 침지하고 7일간 매일 관찰하였다. 시험결과 2,362종(87.0%)의 조추출물은 부식성선충에 전혀 영향을 미치지 않았고, 187종(6.9%)의 조추출물은 부식성선충의 활성을 50% 정도 억제하였고, 95종(3.5%)의 조추출물은 90% 이상 억제를 보였으며, 70종(2.6%)의 조추출물에서는 오히려 부식성선충이 번식되었다. 1차 검정에서 50% 이상 선충억제 활성을 보인 추출물중에서, 25개의 추출물을 임의로 선택하여 2, 3차(부식성선충), 4차(고구마뿌리혹선충 유충) 반복 시험을 수행한 결과, 약 44%의 시료는 지속적인 선충억제 활성을 보였다. 이번 시험에서 선충억제 활성을 나타낸 282개의 식물 조추출물은 앞으로 식물 기원의 살선충 물질 연구시 참고 자료로 활용될 수 있을 것이다.

A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activity against Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of $5,000{\mu}g/ml$ were mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examined daily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influence on the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibited nematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over 50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and with juveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic. The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.

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피인용 문헌

  1. Control Effect of Coffee Ground Compost and Velvet bean Against Root-Knot Nematode, Meloidogyne incognita in Pumpkin vol.20, pp.1, 2016, https://doi.org/10.7585/kjps.2016.20.1.47