Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Electrophoretic karyotype of Flammulina velutipes and its variation among cultivars

팽이버섯의 핵형분석과 균주 사이의 핵형 다양성

  • Lee, Song Hee (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Lee, Mi-Kyoung (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Kim, Na-Ri (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Lee, Chang-Yun (Korea Green Peace 5th Farm) ;
  • Lee, Hyun-Sook (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University)
  • Received : 2014.02.14
  • Accepted : 2014.02.26
  • Published : 2014.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The karyotype of F. velutipes Korean cultivar, Fv 3-6, was compared with those of Japanese cultivars, Fv 0-1, Fv 1-5, Fv 11-1, by CHEF gel electrophoresis. The Korean cultivar, Fv 3-6, showed the difference from the three Japanese cultivars in number and size of chromosomes; the Fv 3-6 had two and one more chromosomes then Fv 0-1 and Fv 11-4, and Fv 1-5 had, respectively. The karyotyping by CHEF gel electrophoresis is quite suitable to define new Korean cultivars against Japanese cultivars.

한국에서 개발된 팽이버섯 균주 Fv 3-6과 일본에서 수집한 팽이버섯 균주 Fv 0-1, Fv 1-5 및 Fv 11-4의 핵형을 CHEF gel electrophoresis 방법으로 분석 하였다. 그 결과 4종류의 균주 모두 chromosome의 전체 길이가 달랐으며, chromosome의 개수 또는 특정 chromosome의 길이가 다른 것을 확인하였다. 특히 Fv 3-6의 경우에는 다른 3 종의 균주와 비교했을 때 Fv 0-1, Fv 11-4 보다는 2개의 chromosome이 더 존재하였고 Fv 1-5 보다는 1개의 chromosome이 더 존재하였으며 핵형패턴이 유사한 일본 수집 균주들과는 다른 핵형패턴을 나타내었다. 이러한 CHEF gel electrophoresis 방법은 품종간의 차이를 SSR이나 ITS 정보를 이용한 방법보다 더 정확하게 구분할 수 있을 것이라고 생각한다.

Keywords

References

  1. Ikekawa T, Maruyama H, Miyano T, Okura A, Sawaskai Y, Naito K, Kawamura K Shiratori K. 1985. Proflamin, a new antitumor agent: preparation, physicochemical properties and antitumor activity. Jpn J Cancer Res. 76:142-148.
  2. Kanatsu N, Terakawa H, Nakanishi K, Watanabe Y. 1963. Flammulin, a basic protein of Flammulina velutipes with antitumour activities. J Antibiot. 16:139-143.
  3. Kim D, Tamai Y, Azuma T, Harada A, Ando A, Sakuma Y, Miura K. 2000. Analysis of the electrophoretic karyotype of Flammulina velutipes. J Wood Sci. 46:466-469. https://doi.org/10.1007/BF00765805
  4. Kim JB, Jeong JI. 2011. Genetic variation in Flammulina velutipes. J Life Sci. 21:1434-1442. https://doi.org/10.5352/JLS.2011.21.10.1434
  5. Knowledgeworks Co. 2012. Strategic plan for the 'Golden Seed Project' through analysis of the seed industry. IPET. Final report.
  6. Kong WS, Yoo CH, Cha DY, Kim DH, Kim KH. 1997. Genetic relationship of Flammulina velutipes based on ribosomal DNA and RAPD analysis. RDA J Indus Crop Sci. 29:28-40.
  7. Leung MYK, Fung KP, Choy YM. 1997. The isolation and characterization of an immunomodulatory and anti-tumor polysaccharide preparation from Flammulina velutipes. Immunopharmacology. 35:255-263. https://doi.org/10.1016/S0162-3109(96)00157-9
  8. Palapala VA, Aimi T, Inatomi S, Morinaga T. 2002. ITS-PCRRFLP method for the distinguishing commercial cultivars of edible mushroom, Flammulina velutipes. J Food Sci. 67: 2486-2490. https://doi.org/10.1111/j.1365-2621.2002.tb08763.x
  9. Park YJ, Kim JK, Kong WS, Song ES, Lee CS, Kim H, Hahn JH, Kang HW, Lee BM. 2010. Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes. Microbiol Res. 165:321-328. https://doi.org/10.1016/j.micres.2009.06.003
  10. Su H, Wang L, Liu L, Chi X, Zhang Y. 2008. Use of inter-simple repeat markers to develop strain-specific SCAR markers for Flammulina velutipes. J Appl Genet. 49:233-235. https://doi.org/10.1007/BF03195619
  11. Tanesaka E, Kinugawa K, Okabe K, Kitamura Y, Ogawa M, Yoshida M. 2003. Electrophoretic Karyotype of Flammulina velutipes and its variation among monokaryotic progenies. Mycoscience. 44:67-69. https://doi.org/10.1007/S10267-002-0082-Z
  12. Zhang R, Hu D, Zhang J, Zuo X, Jiang R, Wang H, Ng TB. 2010. Development and characterization of simple sequence repeat (SSR) markers for the mushroom Flammulina velutipes. JBB. 110:273-275.