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Biochemical Characterization of Agaricus bisporus Dikaryon Strains

양송이 이핵균주의 생화학적 특성 검정

  • Kwon, Hyuk Woo (Department of Microbiology, Dankook University) ;
  • Kim, Jun Young (Department of Microbiology, Dankook University) ;
  • Min, Sung Hwan (Department of Microbiology, Dankook University) ;
  • Choi, Min Ah (Department of Microbiology, Dankook University) ;
  • Oh, Youn-Lee (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural and Medicinal Crop RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural and Medicinal Crop RDA) ;
  • Kim, Seong Hwan (Department of Microbiology, Dankook University)
  • 권혁우 (단국대학교 미생물학과) ;
  • 김준영 (단국대학교 미생물학과) ;
  • 민성환 (단국대학교 미생물학과) ;
  • 최민아 (단국대학교 미생물학과) ;
  • 오연이 (국립원예특작과학원 인삼특작부) ;
  • 공원식 (국립원예특작과학원 인삼특작부) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2014.02.12
  • Accepted : 2014.03.21
  • Published : 2014.03.31

Abstract

Button mushroom (Agaricus bisporus) strains from diverse origins were compared in this study to obtain basic information on their growth and biochemical properties that are helpful for breeding. Among 31 dikaryotic strains tested, most strains showed better mycelial growth on oatmeal agar than on MEA and PDA. Mycelia of the mushroom strains revealed ${\beta}$-glucosidase activity the most clearly among the seven extracellular enzymes tested. All the strains showed protease activity, but ${\beta}$-glucosidase activity was found in 27 strains and xylanase activity was found in 30 strains. The activity of avicelase, CM-cellulase, amylase, and pectinase was detected in less than 20 strains. These results implied that enzymatic characteristics could be used as a criterion of strain selection for breeding study.

본 연구에서는 양송이 육종에 필요한 기본 정보를 얻고자 다양한 출처의 양송이 이핵균주를 대상으로 생육 및 생화학적 특성을 비교 검정하였다. 시험한 31균주 중 대부분의 균주가 Oatmeal agar에서 MEA나 PDA에서 보다 균사생장이 우수하였다. 7가지 세포외효소 활성 비교에서 양송이 균주는 대체로 ${\beta}$-glucosidase가 가장 뚜렷한 활성을 나타내었으며 protease의 활성은 모든 균주가 지니고 있었다. ${\beta}$-glucosidase 활성은 27개 균주에서 xylanase 활성은 30개 균주에서 나타났다. 이에 반해 avicelase, CM-cellulase, amylase, pectinase 활성은 20균주 이하에서만 나타났다. 본 연구 결과는 국내 양송이 육종을 위한 균주 선발 기준 중 하나로 이용될 수 있을 것이다.

Keywords

References

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Cited by

  1. Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus) vol.43, pp.1, 2015, https://doi.org/10.5941/MYCO.2015.43.1.81