The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Co-administration of Aconiti Lateralis Preparata Radix and Cinnamomi Cortex on Osteoclast Differentiation

부자와 육계 병용투여 시 파골세포 분화 억제에 미치는 영향

  • Jung, Gi-Eun (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University) ;
  • Kim, Jung Young (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University) ;
  • Kim, Ji-Hoon (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University) ;
  • Han, Sang-Yong (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University) ;
  • Kim, Yun-Kyung (Department of Herbal Medicine, College of Pharmaceutics, Wonkwang University)
  • 정기은 (원광대학교 약학대학 한약학과 방제학교실) ;
  • 김정영 (원광대학교 약학대학 한약학과 방제학교실) ;
  • 김지훈 (원광대학교 약학대학 한약학과 방제학교실) ;
  • 한상용 (원광대학교 약학대학 한약학과 방제학교실) ;
  • 김윤경 (원광대학교 약학대학 한약학과 방제학교실)
  • Received : 2014.02.24
  • Accepted : 2014.03.15
  • Published : 2014.03.30
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Abstract

Objectives : Aconiti Lateralis Preparata Radix (Aconitum Carmichaeli, AC) and Cinnamomi Cortex (Cinnamomi Cortex, CC) have been treated to elderly for kidney yang enhancement in Korean traditional medicine. In this study, the effects of water extract of AC and CC on RANKL (Receptor Activator for Nuclear Factor ${\kappa}B$ Ligand)-induced osteoclast differentiation were evaluated in culture system. Methods : MTT assay was used to evaluate the potential cytotoxicity of AC and CC extracts in bone macrophage marrows (BMMs) stimulated with M-CSF. TRAP (tartrate-resistant acid phosphatase) staining and TRAP activity were performed to know the inhibitory effect on osteoclast differentiation. The protein expression levels of nuclear factors such as activated T cell(NFAT)c1, c-Fos, MAPKs and ${\beta}$-actin in cell lysates treated with AC and CC extracts were analysed by western blotting. Results : AC, CC extracts and their co-administration inhibited significantly RANKL-induced osteoclast differentiation in BMMs in a dose dependent manner without toxicity. Each AC and CC extracts inhibited the phosphorylation of p38. Also, AC and CC extracts, respectively, inhibited the protein expression of c-Fos and NFATc1 more than Co-administration of AC and CC even if all treatments did. It was observed that RANKL-induced degradation of I-${\kappa}B$ is significantly suppressed by all treatments. Conclusions : Taken together, It was concluded that AC and CC have beneficial effect on osteoporosis by inhibition of osteoclast differentiation. Thus, Atractylodis AC and CC could be a treatment option for osteoporosis.

Keywords

References

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