The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antidepressant-like effects of Nelumbo nucifera leaves extract in chronic mild stress model

만성스트레스 모델에서 하엽추출물의 항우울 효과

  • Kang, Min Gu (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Kim, Young Hwa (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Im, A Rang (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Nam, Byung Soo (Infrastructure Management Team, Korea Institute of Oriental Medicine) ;
  • Chae, Sung Wook (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine) ;
  • Lee, Mi Young (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
  • 강민구 (한국한의학연구원 한의신약개발그룹) ;
  • 김영화 (한국한의학연구원 한의신약개발그룹) ;
  • 임아랑 (한국한의학연구원 한의신약개발그룹) ;
  • 남병수 (한국한의학연구원 인프라운영팀) ;
  • 채성욱 (한국한의학연구원 한의신약개발그룹) ;
  • 이미영 (한국한의학연구원 한의신약개발그룹)
  • Received : 2014.02.12
  • Accepted : 2014.03.20
  • Published : 2014.03.30
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Abstract

Objectives : Chronic mild stress (CMS) model is currently recognized as a better animal model of depression. The purpose of this study was to investigate the antidepressant-like effects of the Nelumbo nucifera leave extract using CMS model. Methods : The antidepressant-like effects of Nelumbo nucifera leaves extract was determined by using animal models of depression. Male ICR mice were divided into four groups: saline-treated normal, without CMS; saline-treated stress control; CMS+ Imipramine(20mg/kg); CMS+Nelumbo nucifera leaves extract(200mg/kg). All mice except the normal group exposed an unpredicted sequence of chronic mild stressors for 5 weeks. The behavior of mice were detected by sucrose preference test, forced swim test and tail suspension test. Then concentration of corticosterone in serum was detected by enzyme immunoassay. Results : Nelumbo nucifera leaves extract administration by daily gavage from the 3rd week exhibited an antidepressant-like effect on CMS-induced depression in mice. Nelumbo nucifera leaves extract administration at dose of 200 mg/kg significantly increased the sucrose consumption, and decreased the immobility durations in forced swim test and tail suspension test. Furthermore the corticosterone level decreased than control group. Conclusions : Chronic mild stress can affect mouse behavior and corticosterone level and cause depression. The present experiments not only further confirm the antidepressant-like effects of Nelumbo nucifera leaves extract in the sucrose preference test, forced swimming test and tail suspension test, but also the improving effects of Nelumbo nucifera leaves extract on the depression-like symptoms in the CMS model. Nelumbo nucifera leaves extract has the antagonism on CMS and produce antidepressive effects.

Keywords

References

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