Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-obesity and Anti-hyperlipidemic Activities of Fermented Coffee with Monascus ruber Mycelium by Solid-State Culture of Green Coffee Beans

고지방 식이를 섭취한 마우스에서 홍국균 균사체-고체발효 원두커피의 비만억제 및 지질저하 효과

  • Sung, Jeehey (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Shin, Ji-Young (R&D Center, CosisBio Co., Ltd.) ;
  • Kim, Hoon (R&D Center, CosisBio Co., Ltd.) ;
  • Baek, Gil-Hun (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Yu, Kwang-Won (Dept. of Food and Nutrition, Korea National University of Transportation) ;
  • Yeon, Jeyeong (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Junsoo (Dept. of Food Science and Biotechnology, Chungbuk National University)
  • 성지혜 (충북대학교 식품생명공학과) ;
  • 신지영 ((주)코시스바이오 기업부설연구소) ;
  • 김훈 ((주)코시스바이오 기업부설연구소) ;
  • 백길훈 (충북대학교 식품생명공학과) ;
  • 유광원 (한국교통대학교 식품영양학과) ;
  • 연제영 (충북대학교 식품생명공학과) ;
  • 이준수 (충북대학교 식품생명공학과)
  • Received : 2013.11.01
  • Accepted : 2013.11.20
  • Published : 2014.03.31
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Abstract

We investigated the anti-obesity and anti-hyperlipidemic effects of extracts of two roasted coffee beans (Vietnam robusta and Ethiopia mocha sidamo G2) and fermented coffee beans with Monascus rubber mycelium (MR) by solid-state culture. C57B/L6 mice were divided into seven groups: normal diet (ND) group, high fat diet (HFD) group, and HFD groups with hot water extracts from Vietnam robusta coffee beans (HFD-VR), MR-fermented Vietnam robusta coffee beans (HFD-VR-MR), MR-fermented Vietnam robusta coffee beans with 10% brown rice (HFD-VR-MR-BR10), Ethiopia mocha sidamo G2 coffee beans (HFD-ES), and MR-fermented Ethiopia mocha sidamo G2 coffee beans (HFD-ES-MR). After 6 weeks, body weight gain and food efficiency ratio were higher in the HFD group, but significantly reduced in the coffee extracts-fed groups. The HFD-ES-MR group showed greater body weight reduction than the HFD-ES group. The serum triglyceride, total cholesterol, and LDL-cholesterol levels as well as the atherogenic index and cardiac risk factor all tended to decrease in groups fed Vietnam robusta coffee extracts compared to the HFD group. These results suggest that Vietnam robusta and Ethiopia mocha sidamo G2 may be used to make functional coffee beverages with anti-obesity and anti-hyperlipidemic activities.

본 연구에서는 두 품종의 원두커피와 이를 Monascus rubber 홍국균으로 발효시킨 원두커피 열수추출물의 체내 지질대사 개선 효과와 항비만 효과를 살펴보기 위하여 고지방 식이를 급여한 쥐에 커피추출물을 6주간 투여한 후 체중, 간 및 부고환 지방조직 무게, 혈액의 지질 농도 함량 등을 분석하였다. 실험 결과 HFD군에서는 고지방 식이로 인하여 체중이 유의하게 증가하였으나 커피추출물을 투여한 모든 실험군에서는 유의적으로 체중이 감소하였고 두 품종 중 에티오피아 모카 시다모 G2종을 홍국균으로 발효하였을 때 더 높은 체중 감소량을 보였다. 혈청 중성지방의 농도는 고지방 식이군이 정상 식이군에 비해 약 2배 정도 증가하였으나 커피추출물을 투여하였을 때 유의적으로 감소하였으며 부고환 지방 콜레스테롤 농도도 유의적으로 감소함을 보였다. LDL 콜레스테롤 저하효과 및 HDL 콜레스테롤 상승효과는 베트남 로부스타종에서만 관찰할 수 있었다. 본 실험결과 커피추출물이 고지방 식이를 급여한 쥐의 체중, 간 및 지방조직의 무게 감소와 더불어 혈장 및 간의 지질대사 개선에 긍정적으로 작용하는 유용한 체내 지질대사 개선 및 항비만 소재가 될 수 있을 것으로 사료된다. 또한 M. rubber 홍국균의 고체배양을 이용한 베트남산 로부스타 및 에티오피아 모카 시다모 G2 발효원두커피는 기능성 커피음료의 좋은 소재가 될 것으로 사료된다.

Keywords

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