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제주도, 한반도 및 대만 내 팔색조의 생태적 서식특성 분석을 위한 유전적 접근

The Genetic Approach on Analyzing the Habitat Characteristics of Fairy Pitta Pitta Nympha Inhabiting Jeju Island, the Korean Peninsula and Taiwan

  • 김은미 (국립산림과학원 난대아열대산림연구소) ;
  • 전연선 (이화여자대학교 환경공학과) ;
  • 김세재 (제주대학교 생물학과) ;
  • 강창완 (한국조류보호협회 제주지회) ;
  • 원현규 (국립산림과학원 난대아열대산림연구소) ;
  • 정길상 (이화여자대학교 에코과학부)
  • Kim, Eun-Mi (Warm-temperate and Subtropical Forest Research Center, Korea Forest Research Institute) ;
  • Jeon, Yeon-Seon (Department of Environment Engineering, Ewha Womans University) ;
  • Kim, Se-Jae (Department of Biology, Jeju National University) ;
  • Kang, Chang-Wan (The Korea Association For Bird Protection Jeju Branch) ;
  • Won, Hyun-Kyu (Warm-temperate and Subtropical Forest Research Center, Korea Forest Research Institute) ;
  • Jeong, Gil-Sang (Department of Ecoscience, Ewha Womans University)
  • 투고 : 2013.11.28
  • 심사 : 2013.12.27
  • 발행 : 2014.02.28

초록

A Fairy Pitta is a bird known to breed only in mainland China, Taiwan, Japan and Korea and is listed as Vulnerable in the IUCN Red List. We carried out a DNA analysis to contribute to conserve the genetic diversity of Fairy Pitta. 32 samples were collected at Jeju Island, the Korean Peninsula and Taiwan from 2004 to 2013 and DNA was extracted from them and several sequences were amplified-it through PCR. And then we performed the population genetic analysis. We found there was a transversion between nucleotide sequences at CO1 gene, while there was no changes at Cyt-b gene. And we confirmed the polymorphism from two genes was caused from genetic drift not from selection. Through this analysis, the group within the Peninsula was found bigger than other two groups based on the analysis of CO1 gene, and the group from Taiwan was found bigger than other two groups through the analysis of Cyt-b gene. The population genetic structure of mitochondria gene of three group was showing CO1 gene had 5 haplotypes and Cyt-b gene had 6 haplotypes. Haplotype 2 in CO1 gene was found in three group and many individuals of samples had this haplotype. Like CO1 gene, haplotype 2 in Cyt-b gene was found in three group and was included in plenty of individuals. Other haplotypes were not overlaped and broke off among the three groups. To prevent from the extinction of Fairy Pitta and to obtain the genetic diversity, we need to compare with other regional group such as Japan, China and perform additional research in the non-breeding area.

키워드

참고문헌

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