대한한의학회지 (The Journal of Korean Medicine)

  • 제35권4호
  • /
  • Pages.83-97
  • /
  • 2014
  • /
  • 1010-0695(pISSN)
  • /
  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지
DOI QR코드

DOI QR Code

The Identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, Using Macroscopic, Microscopic and Physicochemical Methods

  • Yoon, Jee-Hyun (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Park, Ha-Yeong (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Kim, Tae-Ju (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Lee, Seung-Ho (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Kim, Jung-Hoon (Division of Pharmacology, School of Korean Medicine, Pusan National University) ;
  • Lee, Guem-San (Department of Herbology, College of Korean Medicine, Wonkwang University) ;
  • Kim, Hong-Jun (Department of Herbology, College of Korean Medicine, Woosuk University) ;
  • Ju, Young-Sung (Department of Herbology, College of Korean Medicine, Woosuk University)
  • 투고 : 2014.11.29
  • 심사 : 2014.12.31
  • 발행 : 2014.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: This study aimed for establishing the discriminative criteria for Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, which can be confused as 'Mokhyang', because of similar appearances and synonyms. Methods: Morphological characteristics of dried herbal medicines were compared by macroscopic observation. To examine microscopic features of 'Mokhyang', paraffin embedding and the staining by using Ju's method were conducted. Physicochemical experiments were performed using HPLC analysis and antioxidant assay. Results: The types of stem, phyllotaxy and leaf shape were chosen as macroscopic criteria and the size, oil spots, color and root top as well as phloem and central cylinder were compared using microscopic features. The HPLC results showed different amounts of costunolide and dehydrocostuslactone among Aucklandiae Radix, Inulae Radix and Vladimiriae Radix. However, Aristolochiae Radix only contained aristolochic acid. The antioxidant assays also showed that Vladimiriae Radix exhibited strongest antioxidant activity followed by Aucklandiae Radix, Inulae Radix and Aristolochiae Radix. Conclusions: These results demonstrate that macroscopic, microscopic and physicochemical methods would be useful tools for the discrimination of 4 kinds of 'Mokhyang'.

키워드

참고문헌

  1. Han J, Lin HG, Zhong ZY. Study of the effect of exceed critical extracts from Radix Aucklandiae on experimental gastric ulcer model. Journal of Chinese medicinal materials 2005;28(11);1017-9.
  2. Wang HL, Li Y, Bai H, Zhang J. Effect of qi-regulating Chinese medicine on gastrointestinal motility. World Chin J Digestol 2004;12(5):1136-8. https://doi.org/10.11569/wcjd.v12.i5.1136
  3. Korea Food & Drug Administration. The Korean Herbal Pharmacopoeia 2007. Seoul:Shinil Books. 2008:142,378.
  4. Pharmacopoeia Commission of the People's Republic of China. Pharmacopoeia of the People's Republic of China 2010. Beijing:China Medical Science and Technology Press. 2010:57.
  5. Jang IS, Yang CS, Lee SD, Han CH. A review of herbal medicinal products associated with toxic events in Korea. J Korean Oriental Med 2007;28(1):01-10.
  6. Ju YS. Ungok Herbology. 2nd ed. Jeonju: Woosuk Press; 2013, p. 820-3, 837-9, 850-2.
  7. Cosyns JP. Aristolochic acid and Chinese herbs nephropathy. Drug Safety 2003;26:33-48. https://doi.org/10.2165/00002018-200326010-00004
  8. Weng JY, Chen YC. Distinction between Aucklandiae Radix and Inulae Radix. Strait Pharmaceutical Journal 2009;21(1):78-9.
  9. Lee AY, Chun JM, Lee HW, Choo BK, Kim HK. Quantitative and pattern analysis of Aucklandia lappa Decne and Inula heleniumm L. Kor. J. Pharmacogn. 2007;38(3):217-21.
  10. Zheng H. Brief discrimination among Aucklandiae Radix, Inulae Radix and Vladimiriae Radix. Journal of North Pharmacy 2011;8(6):3.
  11. Liu Y, Peng LX, Liu C, Shang YH, Yu QY. HPLC pattern analysis of Aucklandiae Radix, Vladimiriae Radix and Inulae Radix. Chin J Pharm Anal 2006;26(11):1574-6.
  12. Li SF, Pan L, Wu JJ. Distinction of genuine and fake article in Aucklandiae Radix. China Pharmaceuticals 2007;16(4):60.
  13. Oh JH, Hong SP, Choi HY, Park YK, Lee JH. Determination of costunolide from Aucklandiae Radix and substitutive herbs by reserved-phase HPLC. Kor. J. Herbology 2008;23(3):61-6.
  14. Gao F, Fu CM, Hu HL, Wang ZG, Fu S, Mao Q, et al. Differences of metabolic profiling of Vladimiriae Radix before and after simmer processing by HPLC-UV technology. Chinese Traditonal and Herbal Drugs 2013;44(5): 547-51.
  15. Dong S, Liu YT, Peng JB, Jia HM, Zou ZM, Yu CY. Simultaneous HPLC determination of costunolide and dehydrocostuslactone in Xin-ke-shu preparations. Pharmaceutical Crops 2011;2:74-8. https://doi.org/10.2174/2210290601102010074
  16. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-200. https://doi.org/10.1038/1811199a0
  17. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 2006;19:669-75. https://doi.org/10.1016/j.jfca.2006.01.003
  18. Jin YH, Qi DY, Li K, Wang JW. The historical research and herb medications analysis of Aucklandiae and Aristolochiae Radix. Information on Traditional Chinese medicine. 2013;30(1):33-5.
  19. Chen CH, Dickman KG, Moriya M, Zavadil J, Sidorenko VS, Edwards KL, et al. Aristolochic acid-associated urothelial cancer in Taiwan. National Acad Sciences. 2012;109(21):8241-46. https://doi.org/10.1073/pnas.1119920109
  20. Wen Y, Su T, Tang J, Zhang C, Wang X, Cai S, et al. Cytotoxicity of phenanthrenes extracted from Aristolochia contorta in human proximal tubular epithelial cell line. Nephron Exp Nephrol. 2006;103:e95-e102.
  21. Kim CY, Kim YB, Yang BC, Lee JH, Chung MK, Yang KH, et al. Toxicity of Aristolochiae Radix in F344 rats. The Journal of Veterinary Science. 2005;45(1):29-37.
  22. Eom MR, Weon JB, Yun BR, Lee JW, Ma CJ. Quantitative analysis of Aucklandia lappa using costunolide and dehydrocostuslactone. Kor. J. Pharmacogn. 2013;44(3);235-41.

피인용 문헌

  1. Separation and recovery of semi-volatile substances of Cnidii Rhizoma, Aucklandiae Radix and Amomum Fructus by reduced pressure collections and GC-MS vol.33, pp.1, 2014, https://doi.org/10.5806/ast.2020.33.1.11