Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Development of distinction methods for male-sterile and dwarfism herbicide tolerant Zoysia japonica Steud

웅성불임 및 왜성형질의 제초제저항성 들잔디(zoysia japonica Steud.)의 판별기술 개발

  • Lee, Bum Kyu (Biosafety Division, National Academy of Agricultural Science) ;
  • Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Ra, Nu Ri (Biosafety Division, National Academy of Agricultural Science) ;
  • Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kwon, Yong-Ik (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Song, In-Ja (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kim, Chang-Gi (Bio-Evaluation Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Ryu, Tae-Hun (Biosafety Division, National Academy of Agricultural Science) ;
  • Park, Kee Woong (Department of Crop Science, Chungnam National University) ;
  • Lee, Hyo-Yeon (Subtropical Horticulture Research Institute, Jeju National University)
  • 이범규 (국립농업과학연구원 생물안전성과) ;
  • 강홍규 (제주대학교 아열대원예산업연구소) ;
  • 라누리 (국립농업과학연구원 생물안전성과) ;
  • 선현진 (제주대학교 아열대원예산업연구소) ;
  • 권용익 (제주대학교 아열대원예산업연구소) ;
  • 송인자 (제주대학교 아열대원예산업연구소) ;
  • 김창기 (한국생명공학연구원 바이오평가센터) ;
  • 류태훈 (국립농업과학연구원 생물안전성과) ;
  • 박기웅 (충남대학교 식물자원학과) ;
  • 이효연 (제주대학교 아열대원예산업연구소)
  • Received : 2014.06.02
  • Accepted : 2014.08.14
  • Published : 2014.09.30
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Abstract

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world. Concerns about the potential risks of GM crops are also increasing. Safe management for the development and production of GM crops is required according to Living Modified Organism Act in Korea. Planning about the methods, duration, and frequency of environmental monitoring is also required for commercial use of GM crops. GM Zoysia japonica Steud. (event name: JG21) expressing resistance to glufosinate-ammonium has been generated previously. By using gamma ray treatment to JG21 we also developed male sterility and dwarf Z. japonica (event name: JG21-MS). The objective of this study was to establish the monitoring system for environment release of JG21-MS. In this study we extracted RNA from JG21 and JG21-MS and conducted RAPD (random amplified polymorphic DNA) method to distinguish JG21 and JG21-MS.

Keywords

References

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