Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparison of Antioxidant and Anti-Inflammatory Activity on Chestnut, Chestnut Shell and Leaves of Castanea crenata Extracts

밤 과육, 껍질 및 밤나무 잎 추출물에 대한 항산화 및 항염증 활성 비교

  • Kim, Jin Yoon (Laboratory of Veterinary PK and PD, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Seung Yeon (Daegu Technopark) ;
  • Kwon, Hyoung Min (Daegu Technopark) ;
  • Kim, Chan Hyun (Daegu Technopark) ;
  • Lee, Seung Jin (Laboratory of Veterinary PK and PD, College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Seung Chun (Laboratory of Veterinary PK and PD, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Kyung Hwa (Department of Skin and health Care, Susung University)
  • Received : 2013.12.18
  • Accepted : 2014.01.11
  • Published : 2014.02.28
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Abstract

The present study was carried out to evaluate the antioxidant and anti-inflammatory activities of three extracts (hot water, 50% ethanol and mixed solvent;water, ethanol, butylene glycol, propylene glycol) of dried chestnut, chestnut shell, chestnut leaves and dried chestnut leaves obtained from Castanea crenata tree. When conducted DPPH assay, radical scavenging activity of ethanol extract of chestnut shell was the highest with $IC_{50}$ $10.8{\mu}g/mL$ among four extracts from these parts (p < 0.05). In additional results by the xanthine oxidase assay, antioxidant activity showed that water extract of chestnut leaves showed the highest xanthine oxidase inhibitory activity in the tested extracts (p < 0.05). Futhermore, extracts of chestnut shell and leaves exhibited no cytotoxicity in RAW 264.7 cells (p < 0.05). Also, anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited following treatment with extracts of chestnut shell and leaves of 3mg/mL (p < 0.05). These data suggest that extract of chestnut shell have antioxidant and anti-inflamantory activity including chestnut leaves. Therefore, it is considered that Castanea crenata research range and selection of functional material can broaden chestnut shell to other fractions such as chestnut and chestnut leaves.

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References

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