Journal of Ecology and Environment

The Ecological Society of Korea (한국생태학회)
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Effect of N-acetyl-L-cysteine on human chronic myeloid leukemia cells KCL22 treated with mitomycin C

  • Simonyan, Anna (Dept. of Genetics and Cytology, Faculty of Biology, Yerevan State University) ;
  • Hovhannisyan, Galina (Dept. of Genetics and Cytology, Faculty of Biology, Yerevan State University) ;
  • Aroutiounian, Rouben (Dept. of Genetics and Cytology, Faculty of Biology, Yerevan State University) ;
  • Kim, Jin Kyu (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute)
  • Received : 2014.01.27
  • Accepted : 2014.02.17
  • Published : 2014.02.28
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Abstract

The effectiveness of N-acetyl-L-cysteine (NAC) to protect blood cells against Mitomycin C (MMC) induced genotoxicity was investigated in human chronic myeloid leukemia cells (KCL22) using the alkaline comet assay. The comet assay was selected as sensitive and rapid method for analysis of DNA damage and repair in individual cells. NAC treatment alone did not produce any damage in KCL22 cell. But NAC was found to be effective in reducing genotoxic damage in KCL22 cells exposed to MMC. These results confirm the literature data that, given the safety and ability to reduce DNA damage. NAC may be useful to prevent drug-mediated genotoxicity.

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References

  1. Dorr RT. 1988. New findings in in the pharmacokinetic, metabolic and drug-resistance aspects of mitomycin C. Semin Oncol 15: 32-41.
  2. Dusinska M, Collins AR. 2008. The comet assay in human biomonitoring: gene-environment interactions. Mutagenesis 23: 191-205. https://doi.org/10.1093/mutage/gen007
  3. Erdem MG, Cinkilic N, Vatan O, Yilmaz D, Bagdas D, Bilaloglu R. 2012. Genotoxic and anti-genotoxic effects of vanillic acid against mitomycin C-induced genomic damage in human lymphocytes in vitro. Asian Pac J Cancer Prev 13: 4993-4998. https://doi.org/10.7314/APJCP.2012.13.10.4993
  4. Fairbairn DW, Olive PL, O'Neill KL. 1995. The comet assay: a comprehensive review. Mutat Res 339: 37-59. https://doi.org/10.1016/0165-1110(94)00013-3
  5. Furtado RA, de Araújo FR, Resende FA, Cunha WR, Tavares DC. 2010. Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays. J Appl Toxicol 30: 254-259.
  6. Kim JK, Park J, Ryu TH, Nili M. 2013. Effect of N-acetyl-lcysteine on Saccharomyces cerevisiae irradiated with gamma-rays. Chemosphere 92: 512-516. https://doi.org/10.1016/j.chemosphere.2013.02.035
  7. Lewerenz V, Hanelt S, Nastevska C, El-Bahay C, Rohrdanz E, Kahl R. 2003. Antioxidants protect primary rat hepatocyte cultures against acetaminophen-induced DNA strand breaks but not against acetaminophen-induced cytotoxicity. Toxicology 191: 179-187. https://doi.org/10.1016/S0300-483X(03)00256-7
  8. Pawar AA, Vikram A, Tripathi DN, Padmanabhan S, Ramarao P, Jena G. 2009. Modulation of mitomycin C-induced genotoxicity by acetyl-and thio-analogues of salicylic acid. In Vivo 23: 303-307.
  9. Pfuhler S, Wolf HU. 1996. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ Mol Mutagen 27: 196-201. https://doi.org/10.1002/(SICI)1098-2280(1996)27:3<196::AID-EM4>3.0.CO;2-D
  10. Reliene R, Pollard JM, Sobol Z, Trouiller B, Gatti RA, Schiestl RH. 2009. N-acetyl cysteine protects against ionizing radiation-induced DNA damage but not against cell killing in yeast and mammals. Mutat Res 665: 37-43. https://doi.org/10.1016/j.mrfmmm.2009.02.016
  11. Rjiba-Touati K, Ayed-Boussema I, Soualeh N, Achour A, Bacha H, Abid S. 2013. Antioxidant and antigenotoxic role of recombinant human erythropoeitin against alkylating agents: cisplatin and mitomycin C in cultured Vero cells. Exp Biol Med (Maywood) 238: 943-950. https://doi.org/10.1177/1535370213494643
  12. Samuni Y, Goldstein S, Dean OM, Berk M. 2013. The chemistry and biological activities of N-acetylcysteine. Biochim Biophys Acta 1830: 4117-4129. https://doi.org/10.1016/j.bbagen.2013.04.016
  13. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35: 206-221. https://doi.org/10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
  14. Tomasz M. 1995. Mitomycin C: small, fast and deadly (but very selective). Chem Biol 2: 575-579. https://doi.org/10.1016/1074-5521(95)90120-5
  15. Wadhwa S, Mumper RJ. 2013. D-penicillamine and other low molecular weight thiols: review of anticancer effects and related mechanisms. Cancer Lett 337: 8-21. https://doi.org/10.1016/j.canlet.2013.05.027
  16. Yedjou CG, Tchounwou CK, Haile S, Edwards F, Tchounwou PB. 2010. N-acetyl-cysteine protects against DNA damage associated with lead toxicity in HepG2 cells. Ethn Dis 20 (Suppl 1): S101-S103.