The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY (한국해양학회지:바다)

The Korean Society of Oceanography (한국해양학회)
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An Inter-Laboratory Comparison Study on Chlorophyll a Determination in Seawater

해수 중 엽록소 a 측정방법에 대한 실험실 간 비교연구

  • Moon, Cho-Rong (Marine Environmental System Science, University of Science & Technology) ;
  • Kang, Dong-Jin (Marine Environmental System Science, University of Science & Technology) ;
  • Park, Mi-Ok (Department of Oceanography, Pukyong National University) ;
  • Noh, Jae Hoon (Marine Biotechnology Research Department, Korea Institute of Ocean Science & Technology) ;
  • Yoo, In-Jae (Marine Biotechnology Research Department, Korea Institute of Ocean Science & Technology) ;
  • Moon, Jeong-Eon (Korea Ocean Satellite Center, Korea Institute of Ocean Science & Technology) ;
  • Shin, Kyung-Hoon (Department of Earth and Marine Science, Hanyang University) ;
  • Kim, Yun Sook (Ocean Science and Technology Institute, Pohang University of Science and Technology) ;
  • Choi, Joong-Ki (Department of Oceanography, Inha University) ;
  • Suh, Young Sang (Fishery and Ocean Information Division, National Fisheries Research and Development Institute)
  • 문초롱 (과학기술연합대학원대학교 해양환경시스템과학부) ;
  • 강동진 (과학기술연합대학원대학교 해양환경시스템과학부) ;
  • 박미옥 (부경대학교 해양학과) ;
  • 노재훈 (한국해양과학기술원 해양생태계연구부) ;
  • 유신재 (한국해양과학기술원 해양생태계연구부) ;
  • 문정언 (한국해양과학기술원 해양위성센터) ;
  • 신경훈 (한양대학교 해양융합과학과) ;
  • 김윤숙 (포항공과대학교 환경공학부) ;
  • 최중기 (인하대학교 생명해양과학부) ;
  • 서영상 (국립수산과학원 수산해양종합정보과)
  • Received : 2014.01.02
  • Accepted : 2014.01.06
  • Published : 2014.02.28
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Abstract

Chlorophyll a in seawater which is an indicator of phytoplankton biomass and primary production is determined by High Performance Liquid Chromatography (HPLC), Fluorometry and Spectrophotometry. In this study, various methods for chlorophyll a determination in seawater are compared using in situ seawater samples from Korean seas. Three inter-laboratory comparison campaigns were carried out using chlorophyll a standard samples (R0) and in situ seawater samples, collected from the East China Sea (R1) and the East Sea (R2). 6 laboratories by HPLC methods, 4 laboratories by fluorometry, and 3 laboratories by spectrophotometry participated. Precisions, defined as the coefficient of variation (CV) were within 9% in standard samples, 0.8~20% (average: 6.1%) in R1, 4~21% (average: 13.2%) in R2. Discrepancy in three methods was approximately 20% within the range of the sample homogeneity intended the laboratory precision (R1: 8%, R2: 15%). The discrepancy in laboratories was greater than the discrepancy in methods. The chlorophyll a concentrations can be produced within 20% discrepancy in spite of using different methods. It is recommended to consider this 20% discrepancy when using the chlorophyll a data produced by different laboratories and different methods.

해양식물플랑크톤 생물량 및 일차생산력을 추정하기 위한 자료로서 이용되는 해수 중 엽록소 a의 농도는 고성능 액체크로마토그래피(High Performance Liquid Chromatography, HPLC), 형광법(Fluorometry), 분광광도법(Spectrophotometry)등의 분석방법으로 측정된다. 본 연구에서는 여러 실험실에서 이러한 방법들로 분석된 엽록소 a 자료를 통용하기 위한 기초자료로서 활용하고자 우리나라 주변 해역의 현장 시료를 이용한 상호비교실험을 실시하여 엽록소 a 분석 실험실 및 분석 방법간의 차이를 비교하였다. 상호비교실험은 엽록소 a 표준색소(R0) 및 동중국해(R1)와 동해해역(R2)에서 채수된 해수 현장 시료를 이용하여 총 3회 시행되었다. 참가실험실은 각각 HPLC(6개 실험실), 형광법(4개 실험실), 분광광도법(3개 실험실)으로 시료를 분석하였다. 표준색소와 현장시료의 측정결과에서 얻은 실험실 내 정밀도는 변동계수로 평가되었으며, 표준색소(R0)에서 9% 미만, 현장시료에서는 R1: 0.8~20%(평균 6.1%), R2: 4~21%(평균 13.2%)의 정밀도를 보였다. 전체 현장시료의 모집단에서 z-test를 이용하여 이상치를 제거한 측정결과들의 중앙값을 기준 값으로 평가한 HPLC, 형광법, 분광광도법 간의 차이는 20%이내였다. 이러한 차이는 현장시료의 균질성 및 실험실 내의 정밀도를 고려한 차이(R1: 8%, R2: 15%)와 유사한 값을 보였다. 비교결과로 미루어 볼 때, 측정값들간의 차이는 분석방법의 차이보다는 실험실 간 차이가 더 크다고 볼 수 있다. 따라서 각 실험실에서 생산된 자료는 분석 방법이 다르더라도 약 20% 이내에서는 동일한 결과를 생산한다고 볼 수 있으므로 타 실험실에서 서로 다른 방법으로 분석된 엽록소 a의 자료를 활용하는 경우에는 약 20%의 차이에 대한 고려가 필요하다.

Keywords

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