Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Study of Mori Fructus and Dried Mori Fructus Extracts on the Antioxidant Effect and the Inhibitory Effect on Adipocyte Differentiation

상심자와 건조상심자 추출물의 항산화 효과 및 전지방세포 분화억제 효과에 관한 연구

  • Kim, Hyung-Gu (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University) ;
  • Wang, Jing-Hua (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University) ;
  • Lim, Dong-Woo (Departments of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Chae, Hee-Sung (College of Pharmacy, Dongguk University) ;
  • Chin, Young-Won (College of Pharmacy, Dongguk University) ;
  • Choi, Han-Seok (Departments of Endocrinology Medicine, College of Medicine, Dongguk University) ;
  • Kim, Hojun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
  • 김형구 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 왕경화 (동국대학교 한의과대학 한방재활의학과교실) ;
  • 임동우 (동국대학교 한의과대학 한방병리학교실) ;
  • 채희성 (동국대학교 약학대학 약학교실) ;
  • 진영원 (동국대학교 약학대학 약학교실) ;
  • 최한석 (동국대학교 의과대학 내분비내과학교실) ;
  • 김호준 (동국대학교 한의과대학 한방재활의학과교실)
  • Received : 2014.09.17
  • Accepted : 2014.10.13
  • Published : 2014.10.31
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Abstract

Objectives This study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells. Methods 3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including $C/EBP-{\alpha}$, $C/EBP-{\beta}$, $PPAR-{\alpha}$, $PPAR-{\gamma}$, $AMPK-{\alpha}$ were analyzed by Real time PCR. Results Mori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructus extracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of $C/EBP-{\alpha}$, $C/EBP-{\beta}$ genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of $PPAR-{\alpha}$, $PPAR-{\gamma}$, $AMPK-{\alpha}$ genes. Conclusions Mori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes.

Keywords

Acknowledgement

Supported by : 보건산업진흥원

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