The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparative Study of Bojungikgitang in Korea, Japan and China on the Anti-Inflammatory and Anti-Oxidative Effects

보중익기탕 (補中益氣湯)의 한국, 중국, 일본 처방에 대한 항염증 및 항산화 효과 비교 연구

  • Choi, Hye-Min (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry (KOTMIN)) ;
  • Kim, Hui-Hun (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry (KOTMIN)) ;
  • Lee, Hwa-Dong (Korean Traditional Medicine Agency, Korea Promotion Institute for Traditional Medicine Industry (KOTMIN))
  • 최혜민 (한국한방산업진흥원, 한약제제사업단) ;
  • 김희훈 (한국한방산업진흥원, 한약제제사업단) ;
  • 이화동 (한국한방산업진흥원, 한약제제사업단)
  • Received : 2013.12.12
  • Accepted : 2014.01.21
  • Published : 2014.01.30
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Abstract

Objectives : Bojungikgitang (BJT), the Oriental medical prescription has been traditionally used about improvement of immune response and infective disease at Asian nation. In this study, we has compared about the anti-inflammatory and antioxidative effects on BJT of three countries including Korea (Korean Traditional Medicine, KTM), China (Traditional Chinese Medicine, CTM) and Japan (Japanese Traditional Medicine, JTM). Methods : We has basically using LPS-stimulated RAW 264.7 cells. The expression of these inflammatory mediators has measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Also, free radical scavenging assay has tested for anti-oxidative activity as well as the contents of total flavonoid and polyphenol. Results : As a result, we were founded the inhibitory effects of BJT (KTM, CTM, JTM) on LPS-induced production of NO, TNF-${\alpha}$ and IL-6 as well as the anti-oxidative activities. Especially the KTM was most effective in anti-inflammatory and anti-oxidative activities. Conclusions : These results indicate that BJT (KTM, CTM, JTM) has a good anti- inflammatory and anti-oxidative effects. But, there were degree of effects on between pharmacopoeia of the countries. Thus, further study is required that find appropriate methods for extracting as well as establish of standardized processes in order to improve the quality of BJT (KTM, CTM, JTM) as an anti-inflammatory and anti-oxidative agent for treatment of inflammatory diseases.

Keywords

References

  1. Shin HK, Lim BM. Research institutes on traditional medicine in east asian countries and U.S.A. Korean J Orient Med. 2002 ; 8(2) : 67-74.
  2. Shin SS, Kim DC, Kim, SJ, Jung DC, Ryu CR, Sung HJ. The Comparison Reasearch on Oriental Medicinal Materials of Asian Nations Pharmacopoeias. J Assoc Neo Med. 2000 ; 5(2) : 267-76.
  3. Kim EJ, Park HJ, Kim HJ, Kim JH, Ann JY, Lee JH, Kim YK. A monitoring study of marker contents in the Hwagnyeonhaedok-tang ex preparations on the market. Korean J Orient Med Prescription. 2008 ; 16(1) : 95-107.
  4. Kim JH, Seo CS, Shin HK. The comparative study on decoctions of Ssanghwa-tang (Shuanghe-tang) extracted by different extraction conditions. Korean J Orient Med Prescription. 2010 ; 18(2) : 125-34.
  5. Korea Food & Drug Administration (KFDA). Research on Intake of Chinese Medicine by Korean. Hanyang University. 2006 : 91-4.
  6. Seo MJ, Lee KB, Park JH, Hong SH. The Current Trend of Research about Bojungikki-tang. Korean J Orient Med. 2010 ; 16(2) : 83-90.
  7. Kim JH, Lee JK, Shin HK. Analysis of studies on Bojungikgi-tang (Buzhongyiqi-tang) to establish the fundament for Evidence Based Medicine(EBM). Korean J Orient Med. 2011 ; 17(2) : 135-67.
  8. Ikeda S, Kaneko M, Kumazawa Y, Nishimura C. Protective activities of a Chinese medicine, Hochuekki-to, to impairment of hematopoietic organs and to microbial infection. Yakugaku Zasshi. 1990 ; 110 : 682-7. https://doi.org/10.1248/yakushi1947.110.9_682
  9. Ishikawa H, Manabe F, Zhongtao H, Yoshii S, Koiso K. The hormonal response to HCG stimulation in patients with male infertility before and after treatment with hochuekkito. Am J Chin Med. 1992 ; 20 : 157-65. https://doi.org/10.1142/S0192415X92000163
  10. Ito H, Shimura K. Studies on the antitumor activity of traditional Chinese medicines(1). Gan To Kagaku Ryoho. 1985 ; 12 : 2145-8.
  11. Koshikawa N, Imai T, Takahashi I, Yamauchi M, Sawada S, Kansaku A. Effects of Hochu-ekki-to, Yoku-kan-san and Saiko-ka-ryukotsu-borei-to on behavioral despair and acetic acid-induced writhing in mice. Meth Find Exp Clin Pharmacol. 1998 ; 20 : 47-51. https://doi.org/10.1358/mf.1998.20.1.485631
  12. Li XY, Takimoto H, Miura S, Yoshikai Y, Matsuzaki G, Nomoto K. Effect of a traditional Chinese medicine, bu-zhong-yi-qi-tang (Japanese name: Hochu-ekki-to) on the protection against Listeria monocytogenes infection in mice. Immunopharmacol Immunotoxicol. 992 ; 14 : 383-402. https://doi.org/10.3109/08923979209005400
  13. Murakami Y. Clinical effect of hotyuekkito (buzhongyiqitang) on symptoms due to renal ptosis and stress incontinence. Hinyokika Kiyo. 1988 ; 34(10) : 1841-3.
  14. Cho JM, Sato N, Kikuchi K. Prophylactic anti-tumor effect of Hochu-ekki- to (TJ41) by enhancing natural killer cell activity. In Vivo. 1991 ; 5(4) : 389-91.
  15. Harada M, Seta K, Ito O, Tamada K, Li T, Terao H, Takenoyama M, Kimura G, Nomoto K. Concomitant immunity against tumor development is enhanced by the oral administration of a kampo medicine, Hochu-ekki-to (TJ-41: Bu-Zhong-Yi-Qi-Tang). Immunopharmacol Immunotoxicol. 1995 ; 17(4) : 687-703. https://doi.org/10.3109/08923979509037189
  16. Kao ST, Yang SL, Hsieh CC, Yang MD, Wang TF, Lin JG. Immunomodulation of Bu-Zhong-Yi-Qi-Tang on in vitro granulocyte colony-stimulating-factor and tumor necrosis factor-alpha production by peripheral blood mononuclear cells. Immunopharmacol Immunotoxicol 2000 ; 22 : 711-20. https://doi.org/10.3109/08923970009016434
  17. Kao ST, Yeh CC, Hsieh CC, Yang MD, Lee MR, Liu HS, Lin JG. The Chinese medicine Bu-Zhong-Yi-Qi-Tang inhibited proliferation of hepatoma cell lines by inducing apoptosis via G0/G1 arrest. Life Sci. 2001 ; 69(13) : 1485-96. https://doi.org/10.1016/S0024-3205(01)01226-7
  18. Park JY, Yi HS, Park SD. The latest trend of research and clinical usage about Bojungikki-Tang in Japan -searching medical science articles published from 2003 to 2008-. Korean J Orient Med Prescription. 2008 ; 16(2) : 11-29.
  19. Cho W, Nam JW, Kang HJ, Windono T, Seo EK, Lee KT. Zedoarondiol isolated from the rhizoma of Curcuma heyneana is involved in the inhibition of iNOS, COX-2 and pro- inflammatory cytokines via the downregulation of NF-${\kappa}B$ pathway In LPS-stimulated murine macrophages. Int Immunopharmacol. 2009 ; 9(9) : 1049-57. https://doi.org/10.1016/j.intimp.2009.04.012
  20. Aniya Y, Naito A. Oxidative stress- induced activation of microsomal glutathione S-transferase in isolated rat liver. Biochem Pharmacol. 1993 ; 45(1) : 37-42. https://doi.org/10.1016/0006-2952(93)90374-6
  21. Kim CJ, Seo BS, Pothological physiology (I). 5th ed. Sin-Il sangsa. 2006 : 238-9
  22. Liyana-Pathirana CM, Shahidi F. Antioxidant activity of commercial soft and hard wheat (Triticum aestivium L) as affected by gastric pH conditions. J Agric Food Chem. 2005 ; 53(7) : 2433-40. https://doi.org/10.1021/jf049320i
  23. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965 ; 16(3) : 144-58 .
  24. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999 ; 64(4) : 555-9. https://doi.org/10.1016/S0308-8146(98)00102-2
  25. Kim SJ, Kim LC, Kim SH. The Effects of Bojungikgitang & Soeumin-bojungikgitang on the Antitumor Activity against S-180 and the Reduction of Side Effects Induced by Cyclophospha mide. Korean J Orient Physiol Pathol. 1993 ; 18(1) : 119-36.
  26. Ishimitsu R, Nishimura H, Kawauchi H, Kawakita T, Yoshikai Y. Dichotomous effect of a traditional Japanese medicine, bu-zhong-yi-qi-tang on allergic asthma in mice. Int Immunopharmacol. 2001 ; 1(5) : 857-65. https://doi.org/10.1016/S1567-5769(01)00022-4
  27. Kim JH, Lee TH. Effect of Bojungikgitang on Starvation Stress in Mice. Korean J Orient Med Prescription. 2006 ; 14(1) : 133-40.
  28. Song QH, Kobayashi T, Hosoi T, Cyong JC. Effects of tranditional Chinese medicines on murine bone metabolism in a microgravity environment. Am J Chin 2003 ; 31(5) : 739-49. https://doi.org/10.1142/S0192415X03001363
  29. Jang SI, Kim HJ, Kim YJ, Pae HO. Bojungikgitang Inhibits LPS Plus $Interferon-{\gamma}-induced$ Inflammatory Mediators in RAW 264.7 Macrophages. Korean J Orient Med prescription. 2003 ; 11(1) : 115-28.
  30. Park JE, Oh DS, Shin SH, Choi JY, Koo CM, Kim AR, Jung SY, Han KJ, Choi SM. Analysis of Recent Trends in Clinical Research Publications on Acupuncture and Moxibustion Studies. Korean J Orient med. 2007 ; 13(2) : 101-12.
  31. Kim MY, Son IP, Kim SY, Song YS, Chang HK, Yoo SJ, Baek SY, Kang HH, Kim BJ. Anti-inflammatory Effect of Green Tea Cell Water in Activated RAW 264.7 cells with Lipopolysaccharide. Korean J Asthma Allergy Clin Immunol. 2012 ; 32(2) : 115-21.
  32. Kim HH, Park GH, Park KS, Lee JY, An BJ. Anti-oxidant and Anti-inflammation Activity of Fractions from Aster glehni Fr. Schm. Korean J Microbiol Biotechnol. 2010 ; 38(4) : 434-41.
  33. Yoshizawa S, Horiuchi T, Yoshida T, Okuda T. Antitumor promoting activity of (-)-epigallocatechin gallate, the main constituent of Tannin in green tea. Phytother Res. 1987 ; 1(1) : 44-7. https://doi.org/10.1002/ptr.2650010110
  34. Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 1958 ; 181(4617) : 1199-200. https://doi.org/10.1038/1811199a0
  35. Kim SI, Sim KH, Ju SY, Han YS. A Study of Antioxidative and Hypoglycemic Activities of Omija (Schizandra chinensis Baillon) Extract under Variable Extract Conditions. Korean J Food Nutr. 2009 ; 22(1) : 41-7.
  36. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999 ; 26(9-10) : 1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  37. Posadas I, Terencio MC, Guillen I, Ferrandiz ML, Coloma J, Paya M, Alcaraz MJ. Co-regulation between cyclooxygenase-2 and inducible nitric oxide synthase expression in the time course of murine inflammation. Naunyn Schmiedebergs Arch Pharmacol. 2000 ; 361(1) : 98-106. https://doi.org/10.1007/s002109900150