The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Molecular Phylogenetic Study of Nesiohelix samarangae Based on CO-I Gene

동양달팽이 (Nesiohelix samarangae)의 CO-I 유전자를 이용한 분자계통학적 연구

  • Bang, In Seok (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Lee, Yong Seok (Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University)
  • 방인석 (호서대학교 자연과학대학 생명과학과, 기초과학연구소) ;
  • 이용석 (순천향대학교 자연과학대학 생명시스템학과)
  • Received : 2014.11.04
  • Accepted : 2014.12.26
  • Published : 2014.12.31
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Abstract

Previously, we have reported expressed sequence tags (ESTs) analysis on the land snail, Nesiohelix samarangae (Ns). Of these ESTs, we have identified four partial fragments of N. samarangae cytochrome oxydase I (NsCO-I) gene which lead to obtain an 852 bp partial cDNA. Since NsCO-I is one of the best-known molecular phylogenetic markers, we have attempted to conduct comparative in silico analysis by using the NsCO-I gene. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of NsCO-I cDNA indicate that N. samarangae has similarity to three land snails such as Elona quimperiana, Euhadra herklotsi and Euhadra idzumonis.

동양달팽이의 EST 서열 4개의 클론을 어셈블리하여 추출되어진 NsCO-I (partial)서열의 코딩 영역은 852 bp, 284개의 아미노산으로 구성되어 있었다. BLAST 결과를 토대로 하여 유사도가 높은 68개의 서열을 추출 하였으며 MEGA6 프로그램을 통해 clustalW 엔진을 통해 다중서열정열을 수행하고 molecular phylogenetic analysis를 수행한 결과 Heterobranchia, Nudibranchia, Cephalaspidea, Sacoglossa, Pulmonata 등의 카테고리별로 잘 분류 되었으며 Mastigeulota kiangsinensis, Helix aspersa, Cepaea nemoralis, Elona quimperiana, Camaena cicatricosa, Cylindrus obtusus 등 육산패류들과 잘 묶인다는 사실을 확인 할 수 있었다.

Keywords

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