Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of the nutritional compositions of oxidative stress-tolerant transgenic rice and conventional rice

산화 스트레스 내성 형질전환 벼 현미의 주요 영양성분 분석

  • Woo, Hee-Jong (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Shin, Kong-Sik (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lim, Myung-Ho (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Park, Soon Ki (Biosafety Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
  • 우희종 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 신공식 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 임명호 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 박순기 (농촌진흥청 국립농업과학원 생물안전성과)
  • Received : 2014.09.25
  • Accepted : 2014.10.27
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Nutritional assessment of transgenic crops to improve safety evaluations is important for food production. An oxidative stress-tolerant rice was generated by stable insertion of the TC gene-a tocopherol cyclase isolated from tobacco-into the genome of a common variety of japonica colored rice. The nutritional composition of the brown rice grains from the transgenic TC line was compared with that of the parental rice cultivar Heugnambyeo and two different varieties of non-transgenic rice. The results indicate that the analyzed nutritional compositions of the brown grains from the transgenic TC line were within the range of values reported for other commercial lines, and measurements of nutritional compositions were equivalent to those of the non-transgenic rice.

본 연구는 국내에서 개발된 토코페롤 합성 TC 유전자가 형질전환 된 GM 벼와 그 모본인 흑남벼 및 일반벼 품종인 일미벼, 동진벼 현미의 일반성분, 무기질 및 아미노산 함량을 분석하여 조성 차이가 있는지를 비교하였다. 모본벼인 흑남벼와 비교하여 GM 벼 현미의 일반성분 조성 중 수분 함량과 조회분 및 단백질이 다소 높았지만 기존에 보고된 일반품종 벼 현미의 성분함량 범위 안에 포함되었다. 또한 GM 벼 현미에 포함된 아미노산 함량과 무기질 함량은 전반적으로 모본벼과 유의적 차이가 없었다. 따라서 본 실험에 사용된 GM 벼는 유전자 형질전환에 의한 비의도적 영양성분 변화는 없는 것으로 판단된다.

Keywords

References

  1. Abbasi AR, Hajirezaei M, Hofius D, Sonnewald U, Voll LM (2007) Specific roles of $\alpha$- and $\gamma$-tocopherol in abiotic stress responses of transgenic tobacco. Plant Physiol 143:1720-1738 https://doi.org/10.1104/pp.106.094771
  2. AOAC (1990) Official methods of analysis of the Association of Official Analytical Chemists. 15th edition. Association of Official Analytical Chemists. Washington, DC.
  3. Azzi A (2007) Molecular mechanism of alpha-tocopherol action. Free Radic Biol Med 43:16-21 https://doi.org/10.1016/j.freeradbiomed.2007.03.013
  4. Choe JS, Ahn HH, Nam HJ (2002) Comparison of nutritional composition in Korean rices. J Korean Soc Food Sci Nutr 31:885-892 https://doi.org/10.3746/jkfn.2002.31.5.885
  5. Choi H, Moon JK, Park BS, Park HW, Park SY, Kim TS, Kim DH, Ryu TH, Kweon SJ, Kim JH (2012) Comparative nutritional analysis for genetically modified rice, iksan483 and milyang 204, and nontransgenic counterparts. J Korean Soc Appl Biol Chem 55:19-26
  6. Collakova E, DellaPenna D (2003) The role of homogentisate phytyltransferase and other tocopherol pathway enzymes in the regulation of tocopherol synthesis during abiotic stress. Plant Physiol 133:930-940 https://doi.org/10.1104/pp.103.026138
  7. James C (2013) Global Status of Commercialized Biotech/GM Crops: 2013. ISAAA Brief No. 46. ISAAA. Ithaca, NY
  8. Kanwischer M, Porfirova S, Bergmuller E, Dormann P (2005) Alterations in tocopherol cyclase activity in transgenic and mutant plants of Arabidopsis affect tocopherol content, tocopherol composition, and oxidative stress. Plant Physiol 137:713-723 https://doi.org/10.1104/pp.104.054908
  9. KFDA (2011) Korean Food Standards Codex. Korea Food & Drug Administration. Chungwon, Korea
  10. Kim MS, Yang HR, Jeong YH (2004) Mineral contents of brown and milled rice. J Korean Soc Food Sci Nutr 33:443-446 https://doi.org/10.3746/jkfn.2004.33.2.443
  11. Kyoun OY, Oh SH, Kim HJ, Lee JH, Kim HC, Yoon WK, Kim HM, Kim MR (2006) Analyses of nutrients and antinutrients of rice cultivars. Korean J Food Cookery Sci 22:949-956
  12. Lee KP, Kim DH, Kweon SJ, Baek HJ, Ryu TH (2008) Risk assessment and variety registration of transgenic crops. J Plant Biotechnol 35:13-21 https://doi.org/10.5010/JPB.2008.35.1.013
  13. Lee YT, Kim JK, Ha SH, Cho HS, Suh SC (2010) Analyses of nutrient composition in genetically modified $\beta$-carotene biofortified rice. J Korean Soc Food Sci Nutr 39:105-109 https://doi.org/10.3746/jkfn.2010.39.1.105
  14. Lee YT, Lee HM, Ahn BO, Cho HS, Suh SC (2013) Nutritional composition of drought-tolerant transgenic rice. J Korean Soc Food Sci Nutr 42:730-735 https://doi.org/10.3746/jkfn.2013.42.5.730
  15. Machlin LJ, Filipski R, Nelson J, Horn LR, Brin M (1977) Effects of a prolonged vitamin E deficiency in rat. J Nutr 107:1200-1208 https://doi.org/10.1093/jn/107.7.1200
  16. OECD (2004) Series on the safety of novel food and feed No. 10. consensus document on compositional considerations for new varieties of rice (Oryza sativa): key food and feed nutrients and anti-nutrients. OECD. Paris, France
  17. Park SY, Lee SM, Yeo Y, Kweon SJ, Cho HS, Kim JK (2013) Comparison of the nutritional compositions of insect-resistant and glufosinate-tolerant rice and conventional rice. J Appl Biol Chem 56:5-9 https://doi.org/10.3839/jabc.2013.002
  18. Song BH, Kim DY, Kim SK, Kim YD, Choi KS (1988) Distribution of amino acids and fatty acids within the degermed brown rice kernel. J Korean Agric Chem Soc 31:7-12.
  19. USDA/FAS (2014) Grain: world markets and trade, June 2014. Foreign agricultural service/ United states department of agriculture. Washington, DC
  20. Woo HJ, Sohn SI, Shin KS, Kim JK, Kim BG, Lim MH (2014) Expression of tobacco tocopherol cyclase in rice regulates antioxidative defense and drought tolerance. Plant Cell Tiss Organ Cult 119:257-267 https://doi.org/10.1007/s11240-014-0530-4
  21. Yokota T, Igarashi K, Uchihara T, Jishage K, Tomita H, Inaba A, Li Y, Arita M, Suzuki H, Mizusawa H, Arai H (2001) Delayed-onset ataxia in mice lacking alpha-tocopherol transfer protein: Model for neuronal degeneration caused by chronic oxidative stress. Proc Natl Acad Sci USA 98: 15185-15190 https://doi.org/10.1073/pnas.261456098

Cited by

  1. Nutritional Assessment for Grain and Whole Rice Plant of Drought-tolerant GM Rice (Agb0103) vol.30, pp.3, 2018, https://doi.org/10.12719/KSIA.2018.30.3.233