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Penicillium koreense sp. nov., Isolated from Various Soils in Korea

  • You, Young-Hyun (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Cho, Hye Sun (Ginseng Resources Research Laboratory, R&D Headquarters, Korea Ginseng Corporation) ;
  • Song, Jaekyeong (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Dae-Ho (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Houbraken, Jos (CBS-KNAW Fungal Biodiversity Centre) ;
  • Hong, Seung-Beom (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
  • Received : 2014.07.01
  • Accepted : 2014.09.26
  • Published : 2014.12.28

Abstract

During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

Keywords

Taxonomy

Penicillium koreense S.B. Hong, D.H. Kim & Y.H. You, sp. nov. Fig. 2. In section Lanata-Divaricata in subgenus Aspergilloides. Mycobank (MB808759).

Etymology: N.L. neut adj. koreense, pertaining to Korea, from where the novel fungal species was isolated.

Colony diameters after 7 days at 25℃ (in mm): CYA 32-34; MEA 42-48; CREA 32-35; OA 33-38; CYA at 4℃ no growth; CYA at 30℃ 39-43; CYA at 37℃ 15-19.

Colonies on CYA, mostly velvety with a distinct margin and often floccose, radially sulcate, sporulation moderate to strong, conidia en masse grey-green, exudate present as clear droplets; colony reverse beige. Colonies on MEA, velvety to slightly floccose, conidia en masse grey-green, reverse orange-brown, sporulation moderate to strong. On CREA, no exudate, poor growth (KACC 47721T), but some strains grow well (KACC 46682 and 47720), weak to moderate sporulation and no acid production. Colony on OA, velvety to slightly floccose and good sporulation, reverse light orange-brown. No production of ascomata after 2 weeks on incubation.

Conidiophores, predominantly monoverticillate with occasionally a divaricate branch; stipes smooth-walled, slender and long (200-800 × 2.0-3.0 µm), with vesicular apices up to 3.0-5.0 µm, vesicle bearing 6-10 phialides. Phialides ampulliform with a distinct neck (8.0-11.5 × 3.0-4.0 µm). Conidia smooth or finely rough-walled, globose to broadly ellipsoidal 2.5-3.5 (2.9 ± 0.2) × 2.0-3.0 (2.5 ± 0.1).

Type strain: KACC 47721, isolated from a bamboo field soil in Goryeong, Korea. The culture is preserved in a metabolically inactive state (lyophilization and liquid nitrogen storage) in Korean Agricultural Culture Collection in Suwon, Korea. Molecular markers for the species are KM000846 for β-tubulin, and KJ801939 for rDNA-ITS.

Additional strains studied: KACC 46682 (hot pepper field soil), KACC 47720 (maize field soil), and KACC 47722 (coniferous forest soil).

References

  1. Burgstaller W, Strasser H, Woebking H, Schinner F. 1992. Solubilization of zinc oxide from filter dust with Penicillium simplicissimum: bioreactor leaching and stoichiometry. Environ. Sci. Technol. 26: 340-346. https://doi.org/10.1021/es00026a015
  2. Glass NL, Donaldson GC. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61: 1323-1330.
  3. Houbraken J, Lopez-Quintero CA, Frisvad JC, Boekhout T, Theelen B, Franco-Molano AE, Samson RA. 2011. Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter. Int. J. Syst. Evol. Microbiol. 61: 1462-1475. https://doi.org/10.1099/ijs.0.025098-0
  4. Houbraken J, Samson RA. 2011. Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud. Mycol. 70: 1-51. https://doi.org/10.3114/sim.2011.70.01
  5. Katoh K, Standley DM. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30: 772-780. https://doi.org/10.1093/molbev/mst010
  6. Li XM, Liao DX, Xu XQ, Yang Q, Zeng GM, Zheng W, Guo L. 2008. Kinetic studies for the biosorption of lead and copper ions by Penicillium simplicissimum immobilized within loofa sponge. J. Hazard. Mater. 159: 610-615. https://doi.org/10.1016/j.jhazmat.2008.02.068
  7. Mansouri S, Houbraken J, Samson RA, Frisvad JC, Christensen M, Tuthill DE, et al. 2013. Penicillium subrubescens, a new species efficiently producing inulinase. Antonie Van Leeuwenhoek 103: 1343-1357. https://doi.org/10.1007/s10482-013-9915-3
  8. Samson RA, Houbraken J, Thrane U, Frisvad JC, Andersen B. 2010. Food and Indoor Fungi, pp. 210-307. CBS Laboratory Manual Series 2. Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands.
  9. Smith G. 1957. Some new and interesting species of microfungi. Trans. Br. Mycol. Soc. 40: 481-488. https://doi.org/10.1016/S0007-1536(57)80054-0
  10. Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  11. Valix M, Tang JY, Malik R. 2001. Heavy metal tolerance of fungi. Miner. Eng. 14: 499-505. https://doi.org/10.1016/S0892-6875(01)00037-1
  12. White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press, New York.

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