미생물학회지 (Korean Journal of Microbiology)

  • 제50권4호
  • /
  • Pages.285-295
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  • 2014
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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경기도에서 분리한 병원성대장균의 역학적 특성 및 PFGE, 항생제 내성 연구

Analysis of Epidemiological Characteristics, PFGE Typing and Antibiotic Resistance of Pathogenic Escherichia coli Strains Isolated from Gyeonggi-do

  • 김경아 (경기도보건환경연구원 미생물팀) ;
  • 용금찬 (경기도보건환경연구원 미생물팀) ;
  • 정진아 (경기도보건환경연구원 미생물팀) ;
  • 허정원 (경기도보건환경연구원 미생물팀) ;
  • 허은선 (경기도보건환경연구원 미생물팀) ;
  • 박성희 (경기도보건환경연구원 미생물팀) ;
  • 최연숙 (경기도보건환경연구원 미생물팀) ;
  • 윤미혜 (경기도보건환경연구원 미생물팀) ;
  • 이정복 (경기도보건환경연구원 미생물팀)
  • Kim, Kyung-A (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Yong, Kum-Chan (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Jeong, Jin-A (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Huh, Jeong-Weon (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Hur, Eun-Seon (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Park, Sung-Hee (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Choi, Yun-Sook (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Yoon, Mi-Hye (Team of Microbiology, Gyeonggi Institute of Health and Environment) ;
  • Lee, Jong-Bok (Team of Microbiology, Gyeonggi Institute of Health and Environment)
  • 투고 : 2014.08.29
  • 심사 : 2014.11.26
  • 발행 : 2014.12.31
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초록

병원성 대장균은 최근 3년간 경기도에서 발생한 학교급식식 중독의 주된 원인균으로서 그 역학적 특성을 정리하고 PFGE typing과 항생제 감수성 시험으로 분리균주의 특성을 알아보고자 하였다. 2010년부터 2012년까지 19건의 학교급식 식중독에 대한 역학조사보고서를 분석한 결과, 급식의 주된 운영방식은 직영(18건, 95%)이며, 고등학교에서 주로 발생하였다. 계절적 요인으로는 13건(65%)이 6월부터 9월에, 특히 여름방학 후 학기 초인 8월말부터 9월에 더 많이 발생하였다. 수요일(7건, 37%)과 목요일(7건, 37%)에 최초환자가 발생되어, 목요일 7건(37%) 금요일 5건(26%)에 주로 신고가 이루어 졌다. 추정위험에는 월요일(4건, 21%), 화요일(7건, 37%), 수요일(4건, 21%)에 노출되었고, 추정원인체로서 가능성이 높은 식품은 김치류가 50%(5건)를 차지하였다. 분리된 98균주는 EPEC, ETEC, EAEC, EHEC가 각각 50%, 34%, 15%, 1%이며, 항생제 감수성 시험 결과 ampicillin, nalidixic acid, sulfamethoxazole/trimethoprim, tetracycline에 대하여 각각 40%, 37%, 24%, 19%의 내성을 나타내었고, 2, 3세대 cephalosporins계, cabarpenem계, aminoglycosides계, 2세대 quinolones계 항생제에는 감수성이 있었으며, cefalotin, ampicillin/sulbactam, chloramphenicol에는 각각 29%, 25%, 6%가 중간내성을, 그리고 70%에서 한 가지 이상의 항생제에 내성이 관찰되었다. PFGE 분석결과, 57.6%의 similarity로 8개의 group과 32개의 profiles로 분류되었다. 개별적인 소규모 식중독에서 항생제 감수성과 유전자형의 연관성을 찾기가 어려웠으며, 동시에 발생한 대규모 식중독의 경우, 항생제 내성과 PFGE 유형이 매우 유사하여 동일 기원으로부터 유래한 병원성대장균에 의해 식중독 사고가 발생한 것으로 추정이 가능하였다.

This study was conducted to survey the epidemiological characteristics and the isolated strains for pathogenic E. coli which was the major causative organisms for food poisoning occurred at school food services in the Gyeonggi-do area during the past three years. We investigated 19 accidents of food-borne disease outbreaks by pathogenic E. coli at school food services from 2010 to 2012. Food-borne disease outbreaks by pathogenic E. coli were usually occurred at direct management type (18 accidents, 95%) and high schools. For the seasonal factors, 13 accidents (65%) were occurred in June to September, especially the end of August and September after the summer holidays. The first patients were occurred on Wednesday (7 accidents, 37%) and Thursday (7 accidents, 37%), and they were mainly reported on Thursday (7 accidents, 37%) and Friday (5 accidents, 26%). The exposure of risk was estimated in Monday (4 accidents, 21%), Tuesday (7 accidents, 37%) and Wednesday (4 accidents, 21%), and kimchi (5 accidents, 50%) was estimated as the food of the high risk responsible for the outbreaks. 98 isolates of pathogenic E. coli consisted of PEC (50%), ETEC (34%), EAEC (15%), and EHEC (1%). The antibiotic resistance of pathogenic E. coli showed in the descending order of ampicilline (40%), nalidixic acid (37%), trimethoprim/sulfamethoxazole (24%), and tetracycline (19%). The antibiotics of second and third generation cephalosporins, cabarpenem, aminoglycosides, and second generation quinolones had antimicrobial susceptibilities and cefalotin, ampicillin/sulbactam and chloramphenicol showed medium resistance at 29%, 25%, and 6% respectively, and 70% of isolates were resistant to more than one antibiotic. By the PFGE analysis, they were classified into nine major groups and 31 profiles with 57% pattern similarity. It was very difficult to find the correlation of antimicrobial susceptibilities and genotype in the small scale-food poisoning, but the similarity of antimicrobial resistance and PFGE patterns in the large scale-food poisoning enabled the outbreaks to estimate the same pathotype of E. coli derived from identical origins.

키워드

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  3. in Foodborne Outbreak vol.48, pp.4, 2018, https://doi.org/10.4167/jbv.2018.48.4.113
  4. Development of Kinetic Models and Their Applications to Describe the Resistance of Listeria monocytogenes in Napa Cabbage Kimchi to Fermentation Conditions vol.26, pp.1, 2014, https://doi.org/10.3136/fstr.26.53
  5. Combined effect of various salt concentrations and lactic acid bacteria fermentation on the survival of Escherichia coli O157:H7 and Listeria monocytogenes in white kimchi at different temperatures vol.30, pp.12, 2021, https://doi.org/10.1007/s10068-021-00979-9