IMMUNE NETWORK

  • 제14권6호
  • /
  • Pages.289-295
  • /
  • 2014
  • /
  • 1598-2629(pISSN)
  • /
  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
권호 표지
DOI QR코드

DOI QR Code

Reference Values for Peripheral Blood Lymphocyte Subsets in a Healthy Korean Population

  • 투고 : 2014.09.05
  • 심사 : 2014.11.13
  • 발행 : 2014.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Flow cytometric immunophenotyping of peripheral blood lymphocyte subsets is a powerful tool for evaluating cellular immunity and monitoring immune-mediated diseases. The numbers and proportions of blood lymphocyte subsets are influenced by factors such as gender, age, ethnicity, and lifestyle. This study aimed to establish reference ranges for peripheral blood lymphocyte subsets in a healthy Korean population. Blood samples from 294 healthy adults were collected. Lymphocyte subsets were analyzed using a single-platform method with a flow cytometer; white blood cells and lymphocytes were analyzed using an automated hematology analyzer. The mean value of the white blood cell count was $5,665cells/{\mu}l$, and the mean values of the subtype counts (percentages) were as follows: lymphocytes, $1,928cells/{\mu}l$ (35.08%); $CD3^+$ cells, $1,305cells/{\mu}l$ (67.53%); $CD3^+CD4^+$ cells, $787cells/{\mu}l$ (40.55%); $CD3^+CD8^+$ cells, $479cells/{\mu}l$ (25.23%); $CD3^-CD19^+$ cells, $203cells/{\mu}l$ (10.43%); and $CD3^-CD56^+$ cells, $300cells/{\mu}l$ (15.63%). Additionally, the $CD4^+/CD8^+$ ratio was 1.81. In this study, gender and age significantly influenced blood lymphocyte subsets. Our results demonstrate that, as with other populations, a healthy Korean population has its own, region-specific, lymphocyte subset reference ranges.

키워드

참고문헌

  1. Bleesing, J. J., M. R. Brown, S. E. Straus, J. K. Dale, R. M. Siegel, M. Johnson, M. J. Lenardo, J. M. Puck, and T. A. Fleisher. 2001. Immunophenotypic profiles in families with autoimmune lymphoproliferative syndrome. Blood 98: 2466-2473. https://doi.org/10.1182/blood.V98.8.2466
  2. Barnett, D., B. Walker, A. Landay, and T. N. Denny. 2008. CD4 immunophenotyping in HIV infection. Nat. Rev. Microbiol. 6: S7-15. https://doi.org/10.1038/nrmicro1998
  3. Lacombe, F., F. Durrieu, A. Briais, P. Dumain, F. Belloc, E. Bascans, J. Reiffers, M. R. Boisseau, and P. Bernard. 1997. Flow cytometry CD45 gating for immunophenotyping of acute myeloid leukemia. Leukemia 11: 1878-1886. https://doi.org/10.1038/sj.leu.2400847
  4. Lee, B. W., H. K. Yap, F. T. Chew, T. C. Quah, K. Prabhakaran, G. S. Chan, S. C. Wong, and C. C. Seah. 1996. Age- and sex-related changes in lymphocyte subpopulations of healthy Asian subjects: from birth to adulthood. Cytometry 26: 8-15. https://doi.org/10.1002/(SICI)1097-0320(19960315)26:1<8::AID-CYTO2>3.0.CO;2-E
  5. Jalla, S., S. Sazawal, S. Deb, R. E. Black, S. N. Das, A. Sarkar, and M. K. Bhan. 2004. Enumeration of lymphocyte subsets using flow cytometry: Effect of storage before and after staining in a developing country setting. Indian J. Clin. Biochem. 19: 95-99. https://doi.org/10.1007/BF02894264
  6. Doan, C. A., and L. G. Zerfas. 1927. The rhythmic range of the white blood cells in human, pathological leucopenic and leucocytic states, with a study of thirty-two human bone marrows. J. Exp. Med. 46: 511-539. https://doi.org/10.1084/jem.46.3.511
  7. Chng, W. J., G. B. Tan, and P. Kuperan. 2004. Establishment of adult peripheral blood lymphocyte subset reference range for an Asian population by single-platform flow cytometry: influence of age, sex, and race and comparison with other published studies. Clin. Diagn. Lab Immunol. 11: 168-173.
  8. Uppal, S. S., S. Verma, and P. S. Dhot. 2003. Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking. Cytometry B Clin. Cytom. 52: 32-36.
  9. Santagostino, A., G. Garbaccio, A. Pistorio, V. Bolis, G. Camisasca, P. Pagliaro, and M. Girotto. 1999. An Italian national multicenter study for the definition of reference ranges for normal values of peripheral blood lymphocyte subsets in healthy adults. Haematologica 84: 499-504.
  10. Torres, A. J., A. L. Angelo, E. M. Netto, G. P. Sampaio, D. F. Souza, L. A. Inocencio, J. A. Lemos, and C. Brites. 2009. Reference range for T lymphocytes populations in blood donors from two different regions in Brazil. Braz. J. Infect. Dis. 13: 221-225. https://doi.org/10.1590/S1413-86702009000300013
  11. Wong, W. S., A. W. Lo, L. P. Siu, J. N. Leung, S. P. Tu, S. W. Tai, S. C. Lam, and K. F. Wong. 2013. Reference ranges for lymphocyte subsets among healthy Hong Kong Chinese adults by single-platform flow cytometry. Clin. Vaccine Immunol. 20: 602-606. https://doi.org/10.1128/CVI.00476-12
  12. Das, B. R., A. A. Bhanushali, R. Khadapkar, K. D. Jeswani, M. Bhavsar, and A. Dasgupta. 2008. Reference ranges for lymphocyte subsets in adults from western India: influence of sex, age and method of enumeration. Indian J. Med. Sci. 62: 397-406. https://doi.org/10.4103/0019-5359.42725
  13. Bisset, L. R., T. L. Lung, M. Kaelin, E. Ludwig, and R. W. Dubs. 2004. Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland. Eur. J. Haematol. 72: 203-212. https://doi.org/10.1046/j.0902-4441.2003.00199.x
  14. Yaman, A., S. Cetiner, F. Kibar, Y. Tasova, G. Seydaoglu, and I. H. Dundar. 2005. Reference ranges of lymphocyte subsets of healthy adults in Turkey. Med. Princ. Pract. 14: 189-193. https://doi.org/10.1159/000084638
  15. Jiao, Y., A. Qiu, J. Xie, D. Li, and T. Li. 2009. Reference ranges and age-related changes of peripheral blood lymphocyte subsets in Chinese healthy adults. Sci. China C Life Sci. 52: 643-650. https://doi.org/10.1007/s11427-009-0086-4
  16. Saathoff, E., P. Schneider, V. Kleinfeldt, S. Geis, D. Haule, L. Maboko, E. Samky, S. M. de, M. Robb, and M. Hoelscher. 2008. Laboratory reference values for healthy adults from southern Tanzania. Trop. Med. Int. Health 13:612-625. https://doi.org/10.1111/j.1365-3156.2008.02047.x
  17. Bouman, A., M. J. Heineman, and M. M. Faas. 2005. Sex hormones and the immune response in humans. Hum. Reprod. Update. 11:411-423. https://doi.org/10.1093/humupd/dmi008
  18. Oertelt-Prigione, S. 2012. The influence of sex and gender on the immune response. Autoimmun. Rev. 11:A479-A485. https://doi.org/10.1016/j.autrev.2011.11.022
  19. Jentsch-Ullrich, K., M. Koenigsmann, M. Mohren, and A. Franke. 2005. Lymphocyte subsets' reference ranges in an age- and gender-balanced population of 100 healthy adults--a monocentric German study. Clin. Immunol. 116:192-197. https://doi.org/10.1016/j.clim.2005.03.020
  20. Shoormasti, R. S., A. Azimdoost, S. Saghafi, M. Movahhedi, M. T. H. Ashtiani, Z. Pourpak, and M. B. Eslami. 2011. Normal range determination of lymphocytes subsets in normal adults in Iran. Iran J. Allergy Asthma Immunol. 10: 295-298.
  21. Koudmani, M., M. -P. Vasson, and A. Khalil A. 2012. Reference ranges of peripheral blood lymphocyte subsets in Syrian healthy adults by flow cytometry: Influence of age and sex. Int. J. Pharm. Pharm. Sci. 4: 515-518.
  22. Linton, P. J., and K. Dorshkind. 2004. Age-related changes in lymphocyte development and function. Nat. immunol. 5: 133-139.
  23. Castle, S. C. 2000. Clinical relevance of age-related immune dysfunction. Clin. Infect. Dis. 31: 578-585. https://doi.org/10.1086/313947
  24. Camous, X., A. Pera, R. Solana, and A. Larbi. 2012. NK cells in healthy aging and age-associated diseases. J. Biomed. Biotechnol. 2012: 195956.
  25. Weng, N. P. 2006. Aging of the immune system: how much can the adaptive immune system adapt? Immunity 24: 495-499. https://doi.org/10.1016/j.immuni.2006.05.001

피인용 문헌

  1. Cytokine secretion responsiveness of lymphomonocytes following cortisol cell exposure: Sex differences vol.13, pp.7, 2014, https://doi.org/10.1371/journal.pone.0200924
  2. The impact of previous live births on peripheral and uterine natural killer cells in patients with recurrent miscarriage vol.17, pp.1, 2014, https://doi.org/10.1186/s12958-019-0514-7
  3. Outcomes of Haploidentical Stem Cell Transplantation using Total Body Irradiation (600 cGy) and Fludarabine with Antithymocyte Globulin in Adult Patients with Severe Aplastic Anemia: A Prospective Pha vol.26, pp.10, 2020, https://doi.org/10.1016/j.bbmt.2020.06.024
  4. Different Background: Natural Killer Cell Profiles in Secondary versus Primary Recurrent Pregnancy Loss vol.10, pp.2, 2021, https://doi.org/10.3390/jcm10020194
  5. A Critical Review on the Standardization and Quality Assessment of Nonfunctional Laboratory Tests Frequently Used to Identify Inborn Errors of Immunity vol.12, pp.None, 2014, https://doi.org/10.3389/fimmu.2021.721289
  6. Variation of B cell subsets with age in healthy Malawians vol.16, pp.7, 2014, https://doi.org/10.1371/journal.pone.0254320
  7. The unique characteristic in peripheral immune cells in patients with advanced hepatocellular carcinoma vol.120, pp.8, 2014, https://doi.org/10.1016/j.jfma.2020.11.015
  8. Immunosenescence evaluation of peripheral blood lymphocyte subsets in 957 healthy adults from 20 to 95 years old vol.157, pp.None, 2014, https://doi.org/10.1016/j.exger.2021.111615