Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Brugmansia mosaic virus Isolated from Brugmansia spp. in Korea

국내 브루그만시아에서 분리한 Brugmansia mosaic virus의 특성

  • Park, Chung Youl (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Bong-Sub (School of Applied Biosciences, Kyungpook National University) ;
  • Nam, Moon (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Min-A (School of Applied Biosciences, Kyungpook National University) ;
  • Baek, Da-Som (School of Applied Biosciences, Kyungpook National University) ;
  • Bae, Yang Su (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Eun-Hye (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jeong-Sun (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Jong-Yoon (Apple Research Station, National Institute of Horticultural & Herbal Science) ;
  • Lim, Seungmo (Biosystems and Bioengineering Program, University of Science and Technology (UST)) ;
  • Moon, Jae Sun (Biosystems and Bioengineering Program, University of Science and Technology (UST)) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • 박충열 (경북대학교 응용생명과학부) ;
  • 김봉섭 (경북대학교 응용생명과학부) ;
  • 남문 (경북대학교 응용생명과학부) ;
  • 이민아 (경북대학교 응용생명과학부) ;
  • 백다솜 (경북대학교 응용생명과학부) ;
  • 배양수 (경북대학교 응용생명과학부) ;
  • 박은혜 (경북대학교 응용생명과학부) ;
  • 김정선 (국립농업과학원 농업미생물과) ;
  • 최종윤 (국립특작과학원 사과시험장) ;
  • 임승모 (과학기술연합대학원대학교(UST) 시스템생명공학과) ;
  • 문제선 (과학기술연합대학원대학교(UST) 시스템생명공학과) ;
  • 이수헌 (경북대학교 응용생명과학부)
  • Received : 2014.10.28
  • Accepted : 2014.12.03
  • Published : 2014.12.31
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Abstract

In May 2013, an angel's trumpet leaves showing mosaic and malformation symptoms were collected from Suwon city, Gyeonggi-do. An analysis of the collected sample by transmission electron microscopy observation showed filamentous rod particles of 720-800 nm in length. On the basis of the these observations, we performed PCR against three reported Potyviruses (Brugmansia mosaic virus, Colombian datura virus and Brugmansia suaveolens mottle virus), and the sample was positive for BruMV. Pathogenicity and host range test of BruMV was determined by mechanical inoculation. Solanaceae (tobacco, tomato and eggplant) and Amaranthaceae (ground cherry) appeared typical virus symptoms. To determine coat protein of this virus, we designed specific primer pairs, and performed PCR amplification, cloning, and sequencing. Phylogenetic analysis showed that BruMV-SW was most closely related to BruMV isolate SK. Comparison of the BruMV-SW coat protein nucleotide sequences showed 92% to 99% identities to the other BruMV isolates.

2013년 5월, 경기도 수원시에서 모자이크와 기형의 병징을 보이는 천사의 나팔꽃 잎을 채집하였다. 채집한 시료는 전자현미경을 이용하여 검경한 결과 720-800 nm 길이의 사상형 입자가 관찰되었다. 전자현미경 검경 결과에 근거하여 기보고된 3종의 바이러스(Brugmansia mosaic virus, Colombian datura virus, Brugmansia suaveolens mottle virus)에 대하여 RT-PCR을 수행하였으며, BruMV에 양성반응을 보였다. 기계적 접종을 이용하여 BruMV의 병원성과 기주범위를 결정하였다. 가지과(담배, 토마토, 가지)와 비름과(땅꽈리)에서 전형적인 바이러스 병징이 나타났다. BruMV의 외피단백질을 결정하기 위하여 특이적 프라이머를 설계하였고, PCR, 클로닝, 시퀀싱을 수행하였다. 계통수 분석 결과, BruMV-SW는 BruMV SK와 가장 유사한 것으로 나타났다. 외피단백질을 이용한 뉴클레오타이드 상동성 비교에서는 BruMV 분리주와 92%와 99%의 상동성을 보였다.

Keywords

References

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