Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Virulence Factor Profiles of Escherichia coli O157:H7 Bacteriophage Isolates from Sewage and Livestock Stools

하수와 가축분변에서 분리된 대장균 O157:H7 박테리오파지의 병원성인자 프로파일

  • Seo, Jina (School of Food Science and Technology, Chung-Ang University) ;
  • Seo, Dong Joo (School of Food Science and Technology, Chung-Ang University) ;
  • Lee, Min Hwa (School of Food Science and Technology, Chung-Ang University) ;
  • Jeon, Su Been (School of Food Science and Technology, Chung-Ang University) ;
  • Oh, Hyejin (School of Food Science and Technology, Chung-Ang University) ;
  • Oh, Mi Hwa (National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Changsun (School of Food Science and Technology, Chung-Ang University)
  • Received : 2014.09.27
  • Accepted : 2014.11.21
  • Published : 2014.12.31
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Abstract

The aim of study was to investigate the virulence profile of Escherichia coli O157:H7 bacteriophages isolated from sewage and livestock stools. Among 23 E. coli O157:H7 bacteriophages, 14 strains were isolated from sewage and 9 were from animal stools collected from 10 livestock farms in Korea. For each bacteriophage DNA sample, the presence of stx1, stx2, eae, aafII, ial, elt, estI, estII, astA, afa, and cnf was examined by polymerase chain reaction. The detection rate of eae, stx2, estI, astA, and ial was 100%, 69.6%, 13.0%, 13.0%, 8.7%, respectively. While all E. coli O157:H7 bacteriophages isolated from stools carried eae+stx2, stx2+eae, eae+astA, eae, stx2+eae+estI, eae+estI, stx2+eae+ial, and eae+ial were observed in bacteriophages isolated from sewage. As several plasmid-carrying virulence factors (estI, astA, and ial) were found in E. coli O157:H7 bacteriophages obtained from sewage and stools, the microbial safety of bacteriophages should be investigated in further study.

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