Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Analysis of Contamination Sources of Staphylococcus aureus Related to Perilla Leaves Using Multi-Locus Variable Number of Tandem Repeat Analysis (MLVA)

Multi-Locus Variable Number of Tandem Repeat Analysis (MLVA)를 이용한 들깻잎 중 Staphylococcus aureus의 오염원분석

  • Kim, Se-Ri (Microbial Safety Team, Department of Crop Life Safety, NAAS, RDA) ;
  • Shim, Won-Bo (School of Physics and Chemistry, Gwangju Institute of Science and Technology) ;
  • Han, Noo Ri (Microbial Safety Team, Department of Crop Life Safety, NAAS, RDA) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 심원보 (광주과학기술원) ;
  • 한누리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Received : 2014.07.21
  • Accepted : 2014.10.08
  • Published : 2014.12.31
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Abstract

To investigate the prevalence of Staphylococcus aureus (S. aureus) during production of perilla leaves, a total of 261 samples, including water, soil, surroundings of cultivation and packing, workers, and perilla leaves, was examined.. To trace the contamination sources of S. aureus related to perilla leaves, MLVA (Multi-Locous Variable number of tandem repeat Analysis), which is a very efficient method to discriminate strains with minimum molecular biology equipment was applied to S. aureus isolated from perilla leaves farms. S. aureus was isolated in perilla leaves from 9 of 38 farms at 0-2.92 log CFU/g. S. aureus was also found in working environment, including packing vinyl, worker clothes, irrigation water and hands. The patterns of MLVA of isolates from perilla leaves matched with those of isolates from packing table, irrigation water, packing vinyl, and hands. The isolates were successfully examined and determined by MLVA, thus elucidating S. aureus source and spread.

본 연구는 들깻잎과 들깻잎 생산 환경을 대상으로 S. aureus를 분리하고 MLVA를 이용해 분리된 S. aureus의 오염경로를 분석하였다. 그 결과, 들깻잎에서 S. aureus의 검출빈도는 7.9% (9/114)였고 검출수준은 $0{\sim}2.92{\log}_{10}CFU/g$였으며 2곳의 농가에서 S. aureus가 $2.00{\log}_{10}CFU/g$ 이상으로 검출되었다. S. aureus가 분리된 5농가를 대상으로 들깻잎과 직접 접촉하는 시료에서 S. aureus를 분리하였으며 5 농가 중 3농가의 작업환경 (농가의 포장대, 포장비닐, 작업자 손, 복장 등)에서 S. aureus가 검출되었다. 들깻잎과 생산 환경에서 분리된 S. aureus를 대상으로 MLVA를 수행했을 때 포장대, 작업자의 손, 포장비닐 및 관개용수에서 분리된 균주와 들깻잎에서 분리된 S. aureus가 동일한 패턴을 보여 들깻잎의 오염은 이들 시료와 연관되어 있을 것으로 추정된다. 따라서 MLVA를 이용한 유전자 typing은 농산물이나 식품 중 S. aureus의 오염원을 신속하고 경제적으로 구명하는데 활용할 수 있을 것으로 판단된다.

Keywords

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