Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Keratinase Production by Recalcitrant Feather Degrading Pseudomonas Geniculata and Its Plant Growth Promoting Activity

난분해성 우모분해 Pseudomonas geniculata에 의한 케라틴 분해효소 생산 및 식물성장 촉진 활성

  • Go, Tae-Hun (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Lee, Sang-Mee (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Lee, Na-Ri (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Hong, Chang-Oh (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University) ;
  • Son, Hong-Joo (College of Natural Resources & Life Science, Life and Industry Convergence Institute, Pusan National University)
  • 고태훈 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 이상미 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 이나리 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 정성윤 (대구가톨릭대학교 의생명과학과) ;
  • 홍창오 (부산대학교 생명자원과학대학 및 생명산업융합연구원) ;
  • 손홍주 (부산대학교 생명자원과학대학 및 생명산업융합연구원)
  • Received : 2013.04.03
  • Accepted : 2013.05.30
  • Published : 2013.11.29
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Abstract

We investigated the optimal conditions for keratinase production by feather-degrading Pseudomonas geniculata H10 using one variable at a time (OVT) method. The optimal medium composition and cultural condition for keratinase production were determined to be glucose 0.15% (w/v), beef extract 0.08% (w/v), $KH_2PO_4$ 0.12% (w/v), $K_2HPO_4$ 0.02% (w/v), NaCl 0.07% (w/v), $MgSO_4{\cdot}7H_2O$ 0.03%, $MgCl_2{\cdot}6H_2O$ 0.04% along with initial pH 10 at 200 rpm and $25^{\circ}C$, respectively. The production yield of keratinase was 31.6 U/ml in an optimal condition, showing 4.6-fold higher than that in basal medium. The strain H10 also showed plant growth promoting activities. This strain had ammonification activity and produced indoleacetic acid (IAA), siderophore and a variety of hydrolytic enzymes such as protease, lipase and chitinase. Therefore, this study showed that P. geniculata H10 could be not only used to upgrade the nutritional value of feather wastes but also useful in situ biodegradation of feather wastes. Moreover, it is also a potential candidate for the development of biofertilizing agent applicable to crop plant soil.

Keywords

References

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