대한환경공학회지 (Journal of Korean Society of Environmental Engineers)

  • 제35권9호
  • /
  • Pages.654-659
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  • 2013
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  • 1225-5025(pISSN)
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  • 2383-7810(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
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액상 당밀과 질산성 질소의 C/N 비율에 따른 Pseudomonas sp. KY1의 탈질 능력 및 그 최적비율에 관한 연구

Enhancement of Denitrification Capacity of Pseudomonas sp. KY1 through the Optimization of C/N ratio of Liquid Molasses and Nitrate

  • 이규연 (서울대학교 건설환경공학부) ;
  • 이병선 (한국농어촌공사 농어촌연구원) ;
  • 신도연 (한국지질자원연구원 희유자원연구센터) ;
  • 최용주 (스탠포드대학교 토목환경공학과) ;
  • 남경필 (서울대학교 건설환경공학부)
  • Lee, Kyuyeon (Department of Civil and Environmental Engineering, Seoul National University) ;
  • Lee, Byung Sun (Rural Research Institute, Korea Rural Community Corporation) ;
  • Shin, Doyun (Rare Metals Research Center, Korea Institute of Geoscience and Mineral Resources) ;
  • Choi, Yongju (Department of Civil and Environmental Engineering, Stanford University) ;
  • Nam, Kyoungphile (Department of Civil and Environmental Engineering, Seoul National University)
  • 투고 : 2013.08.19
  • 심사 : 2013.09.23
  • 발행 : 2013.09.30
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초록

본 연구에서는 액상당밀을 외부탄소원으로 이용하는 탈질미생물 Pseudomonas sp. KY1의 탈질능력을 확인하고 최적의 C/N 비율을 도출하였다. 회분식 실험 결과, C/N 비율 3/1에서 $0.0263hr^{-1}$의 유사1차반응상수가 도출되었고, 이 비율에서 100 mg-N/L의 초기 질산성질소는 실험시작 후 약 100시간 이내에 약 80%의 제거율을 보였다. C/N 비율 3/1의 컬럼 실험에서 초기 질산성질소 농도 100 mg-N/L의 오염수(유속 0.3 mL/min)는 실험시작 후 172시간(35 PV) 이후부터 실험 종료 시(62 PV)까지 최대 95%의 탈질효율을 보였고, 이 비율에서 2차 오염원으로 작용할 수 있는 잔류당밀의 농도를 최소화(125~180 mg-COD/L) 할 수 있었다.

This study was conducted to identify an optimal ratio of carbon to nitrogen (C/N ratio) for denitrification of nitrate using molasses as an external carbon source. A series of batch and column tests was conducted using an indigenous bacterium Pseudomonas sp. KY1 isolated from a nitrate-contaminated soil. For the initial nitrate-nitrogen concentration of 100 mg-N/L, batch test results indicated that C/N ratio of 3/1 was the optimal ratio with a relatively high pseudo-first-order reaction constant of $0.0263hr^{-1}$. At C/N ratio of 3/1, more than 80% of nitrate-nitrogen concentration of 100 mg-N/L was removed in 100 hrs. Results of column tests with a flow velocity of 0.3 mL/min also indicated that the C/N ratio of 3/1 was optimal for denitrification with minimizing remaining molasses concentrations. After 172 hrs of column operation (35 pore volumes) with an influent nitrate-nitrogen concentration of 100 mg-N/L, the effluent met the drinking water standard (i.e., 10 mg $NO_3$-N/L).

키워드

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