KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Moisturization and Transdermal Penetration Characteristics of PEGimpregnated Aloe vera Gel from DIS Processing

DIS에 의한 Polyethylene Glycol 함침 알로에 베라 겔의 보습 및 경피흡수 특성

  • Kwon, Hye Mi (Department of Bioengineering and Technology, Kangwon National University) ;
  • Hur, Won (Department of Bioengineering and Technology, Kangwon National University) ;
  • Lee, Shin Young (Department of Bioengineering and Technology, Kangwon National University)
  • 권혜미 (강원대학교 생물공학과) ;
  • 허원 (강원대학교 생물공학과) ;
  • 이신영 (강원대학교 생물공학과)
  • Received : 2013.08.12
  • Accepted : 2013.09.18
  • Published : 2013.10.30
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Abstract

This study was carried out to investigate the in vitro and in vivo moisturizing properties and percutaneous absorption of PEG-impregnated Aloe vera gel. The PEG-i-Aloe gel was obtained from dewatering and impregnation by soaking (DIS) of Aloe vera leaf slice. The moisturizing property of the obtained sample was evaluated by moisture determination using gravimetric method in desiccator under different RH% and by water sorption-desorption test on human skin. The transdermal penetration characteristics of PEG-i-Aloe gel was investigated by Franz diffusion cell in vitro transdermal absorption method. PEG-i-Aloe gel had high moisture retention ability and could significantly lead the enhancing skin hydration status as well as reducing the skin water loss due to the film formation as a skin barrier. The skin penetration rate of PEGi- Aloe gel at steady state was 9.76 ${\mu}g/(h{\cdot}cm^2)$ and the quantity of the transdermal absorption was 144 ${\mu}g/cm^2$ in 9 hr. The penetration mechanism was well fitted with Higuchi model ($R^2$ = 0.974-0.994). The results show that PEG-i-Aloe gel has the significant moisturizing effect and strong penetration of the animal skin. It could be used as the moisturizing additive in cosmetic skin products.

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References

  1. Chen, R. H. and W. Y. Chen (2003) Skin hydration effects, film formation time, and physicochemical properties of moisture mask containing Monostroma nitidium water-soluble mucilage. J. Cosmet. Sci. 54: 9-20.
  2. Kraft, J. N. and C. W. Lynde (2005) Moisturizers: What they are and a practical approach to product selection. Skin Therapy Letter 10: 1-8.
  3. Wei, X., Y. Liu, J. Xiao, and Y. Wang (2009) Protective effects of tea polysaccharides and polyphenols on skin. J. Agric Food Chem. 57: 7757-7762. https://doi.org/10.1021/jf901340f
  4. Eom, S. Y., C. B. Chung, Y. S. Kim, J. H. Kim, K. S. Kim, Y. H. Kim, S. H. Park, Y. I. Hwang, and K. H. Kim (2006) Cosmeceutical properties of polysaccharides from the root bark of Ulmus davidiana var. japonica. J. Cosmet. Sci. 57: 355-367.
  5. Lynde, C. W. (2001) Moisturizers: What they are and how they work? Skin Therapy Lett. 6: 3-5.
  6. Kapoor, S. and S. Saraf (2010) Assessment of viscoelasticity and hydration effect of herbal moisturizers using bioengineering techniques. Pharmacogn Mag. 6: 298-304. https://doi.org/10.4103/0973-1296.71797
  7. Shweta, K. and S. Swarnlata (2010) Formulation and evaluation of moisturizer containing herbal extracts for the management of dry skin. Phcog. J. 2: 409-418. https://doi.org/10.1016/S0975-3575(10)80024-0
  8. Gediya, S. K., R. B. Mistry, U. K. Patel, M. Blessy, and H. N. Jain (2011) Herbal Plants: Used as a cosmetics. J. Nat. Prod. Plant Resour. 1: 24-32.
  9. IASC_Aloe_vera_A_Scientific_Primer.pdf. http://www.iasc.org/pdfs/ (2013)
  10. Eshun, K. and Q. He (2004) Aloe vera: A valuable ingredient for the food, pharmaceutical and cosmotic industries, A review. Critical Reviews in Food Science and Nutrition 44: 91-96. https://doi.org/10.1080/10408690490424694
  11. Christaki, E. V. and P. C. Florou-Paneri (2010) Aloe vera: A plant for many uses. J. Food Agric. Environ. 8: 245-249.
  12. Grindlay, D. and T. Reynolds (1986) The Aloe vera phenomenon: A review of properties and modern uses of the leaf parenchyma gel. J. Ethnopharmacol. 16: 117-151. https://doi.org/10.1016/0378-8741(86)90085-1
  13. KFDA (2011) Health Functional Food Code. II.2.4.5. Aloe gel. pp. 99.
  14. Devi, R. and Y. M. Rao (2005) Cosmeceutical application of Aloe gel. Natural Product Radiance 4: 322-327.
  15. Basmatker, G., N. Jais, and F. Daud (2011) Aloe vera: A valuable multifunctional cosmetic ingredient. Int. J. Med. Arom. Plants 1: 338-341.
  16. He, Q., L. Changhong, E. Kojo, and Z. Tian (2005) Quality and safety assurance in the processing of Aloe vera gel juice. Food Control, 16: 95-104. https://doi.org/10.1016/j.foodcont.2003.12.001
  17. Bozzi, A., C. Perrin, S. Austin, and F. Arce (2007) Quality and authenticity of commercial aloe vera gel powders. Food Chem. 103: 22-30. https://doi.org/10.1016/j.foodchem.2006.05.061
  18. Kwon, H. M., W. Hur, and S. Y. Lee (2013) Preparation and characterization of PEG-modified Aloe gel through DIS processing of Aloe vera leaf slice. KSBB Journal, accepted (Oct. 29, 2013).
  19. Yang, S., Z. Guo, Y. Zhou, L. Zhou, Q. Xue, F. Miao, and S. Qin (2010) Synthesis and moisture absorption and retention activities of a carboxymethyl and a quaternary ammonium derivertive of a,atrehalose. Carbohydrate Research 345: 120-123. https://doi.org/10.1016/j.carres.2009.09.024
  20. Tagami, H., Y. Kanamaru, K. Inoue, S. Suehisa, F. Inoue, K. Iwatsuki, K. Yoshikuni, and M. Yamada (1982) Water sorption-desorption test of the skin in vivo for functional assessment of the stratum corneum. J. Investigative Dermatology 78: 426-428.
  21. Sindhvananda, J., P. Gritiyarangsan, P. Rungrairatanaroij, and P. Kullavanijaya (1993) Hygroscopicity and water-holding capacity of moisturizing agents: A single- application in vivo study. J. Soc. Cosmet. Chem. 44: 279-288.
  22. Eberendu, A. R., G. Luta, J. A. Edwards, B. H. McAnalley, and B. Davis (2005) Quantitative colorimetric analysis of aloe polysaccharides as a measure of Aloe vera quality in commercial products. J. AOAC Int. 88: 684-691.
  23. Salerno, C., A. M. Carlucci, and C. Bregni (2010) Study of in vitro drug release and percutaneous absorption of fluconazole from topical dosage forms. AAPS Pharm. Sci. Tech. 11: 986-993. https://doi.org/10.1208/s12249-010-9457-1
  24. Akhtar, N., M. U. Rehman, H. M. S. Khan, F. Rasool, T. Saeed, and G. Murtaza (2011) Penetration enhancing effect of polysorbate 20 and 80 on the in vitro percutaneous absorption of l-ascorbic acid. Trop. J. Pharm. Res. 10: 281-288.
  25. Higuchi, W. I. (1967) diffusional models useful in biopharmaceutics-drug release rate process. J. Pharm. Sci. 56: 315-324. https://doi.org/10.1002/jps.2600560302
  26. Kobayashi, R., M. Takisada, T. Suzuki, K. Kirimura, and S. Usam (1997) Neoagarobiose as a novel moisturizer with whitening effect. Biosci. Biotech. Biochem. 61: 162-163. https://doi.org/10.1271/bbb.61.162
  27. Sagiv, A. E. and Y. Marcus (2003) The connection between in vitro water uptake and in vivo skin moisturization. Skin Research and Technology 9: 306-311. https://doi.org/10.1034/j.1600-0846.2003.00035.x
  28. Meadows, T. P. (1980) Aloe as a humectant in new skin preparations. Cosmetics Toiletries 95: 51-56.
  29. Wang, X., X. Xu, and L. Zhang (2008) Thermally induced conformation transition of triple-helical lentina in NaCl aqueous solution. J. Phys. Chem. B 112: 10343-103. https://doi.org/10.1021/jp802174v
  30. Dal'Belo, S. E., L. R. Gaspar, and P. M. Maia Campos (2006) Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques. Skin Res. Technol. 12: 241-246. https://doi.org/10.1111/j.0909-752X.2006.00155.x
  31. Atherton, P. (1998) Aloe Vera: magic or medicine? Nurs Stand. 12: 49-52, 54.
  32. Ren, H. Y., Q. E. Wang, Y. M. Dong, and H. Meng (2012) Skincare characteristic of active polysaccharide from Aloe vera (in Chinese). Drug Evaluation Research 35: 431-434.
  33. Madan, J. and R. Singh (2010) Formulation and evaluation of Aloe vera topical gels. Int. J. Ph. Sci. 2: 551-555.