제주 근해에서 채집된 나망곰치, Gymnothorax minor (뱀장어목, 곰치과) 엽상자어의 분자동정 및 형태기재

Molecular Identification and Morphological Description of Gymnothorax minor (Muraenidae, Anguilliformes) Leptocephali off Jeju Island

  • 이승종 (국립수산과학원 아열대수산연구센터) ;
  • 지환성 (부경대학교 자원생물학과) ;
  • 김진구 (부경대학교 자원생물학과)
  • Lee, Seung-Jong (Subtropical Fisheries Research Center, NFRDI) ;
  • Ji, Hwan-Sung (Department of Marine Biology, Pukyong National University) ;
  • Kim, Jin-Koo (Department of Marine Biology, Pukyong National University)
  • 투고 : 2012.07.19
  • 심사 : 2013.02.25
  • 발행 : 2013.06.30

초록

한국 제주도에서 채집된 곰치과(Muraenidae) 엽상자어 2개체 (전장 36.2~69.2 mm)를 대상으로 mtDNA 12S rRNA 724 base pair 염기서열을 분석한 결과, 한국 통영에서 채집된 나망곰치(Gymnothorax minor) 성어와 100% 일치하였다. 나망곰치 엽상자어는 전체 근절수가 137~139개, 등지느러미 앞 근절수가 32개, 항문앞 근절수가 82~86개, 전장에 대한 체고가 10% 이상인 형태적 특징을 나타내었다.

Two leptocephali (36.2~69.2 mm in total length) of the family Muraenidae were collected off Jeju Island, Korea. They were identified using 742 base pairs of mitochondrial DNA 12S rRNA sequences, and their morphological description were presented. The two leptocephali were identified to Gymnothorax minor based on genetic distance (d=0.000), being well consistent with that of G. minor adult collected off Tongyeong. Gymnothorax minor leptocephali are characterized by morphological combinations: total myomeres 137~139, predorsal myomeres 32, preanal myomeres 82~86, body depth in total length more than 10%.

키워드

참고문헌

  1. 지환성.김진구. 2010. 남해에서 채집된 바다뱀과 엽상자어(Leptocephali) 의 형태 및 분자동정. 한국어류학회지, 22: 279-284
  2. 지환성.이수정.김진구. 2011. 날붕장어 (Echelus uropterus) 엽 상자어의 분자동정, 개체발생 및 진화학적 고찰. 한국어류학회지, 23: 217-224.
  3. Castle, P.H.J. 1965. Muraenid leptocephali in Australasian Waters. Trans. Roy. Soc. N.Z. Zool., 7: 57-84.
  4. Castle, P.H.J. 1984. Notacanthiformes and Anguilliformes: Development. In: Moser, H.G., W.J. Richards, D.M. Cohen, M.P. Fahay, A.W. Kendall and S.L. Richardson (eds.), Ontogeny and Systematics of Fishes. Amer. Soc. Ichthyol. Herpetol., Lawrence, pp. 62-93.
  5. Ji, H.S., H.W. Lee, B.K. Hong and J.K. Kim. 2012. Four newly reported ophichthid leptocephali species revealed by mitochondrial 12S rDNA, with implications of their occurrence in Korea. Anim. Cells. Syst., 16: 415-424. https://doi.org/10.1080/19768354.2011.652168
  6. Kendall, A.W., E.H. Ahlstrom and H.G. Moser. 1984. Early life history stages of fishes and their characters. In: Moser, H.G., W.J. Richards, D.M. Cohen, M.P. Fahay, A.W. Kendall and S.L. Richardson (eds.), Ontogeny and Systematics of Fishes. Amer. Soc. Ichthyol. Herpetol., Lawrence, pp. 11-22.
  7. Kim, S.Y., H.S. Ji and J.K. Kim. 2012. Review of the scientific name and redescription of the banded moray eel, previously reported as Gymnothorax reticularis (Muraenidae, Anguilliformes) in Korea. Korean J. Ichthyol., 24: 292-296.
  8. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleo-tide sequences. J. Mol. Evol., 16: 111-120. https://doi.org/10.1007/BF01731581
  9. Ko, H.L., Y.T. Wang, T.S. Chiu, M.A. Lee, M.Y. Leu, K.Z. Chang, W.Y. Chen and K.T. Shao. 2013. Evaluating the accuracy of morphological identification of larval fishes by applying DNA Barcoding. PLoS ONE, 8(1): e53451. doi:10.1371/ journal.pone.0053451.
  10. Nair, R.V. 1947. On the metamorphosis of two leptocephali from the Madras plankton. Proc. Indian Acad. Sci. Sect. B, 25: 1-14.
  11. Nelson, J.S. 2006. Fishes of the world. 4th ed. John Wiley & Sons, Inc., New Jersey, 601pp.
  12. Smith, D.G. 1989. Family Muraenidae: leptocephali. In: Böhlke, E.B. (ed.). Fishes of the Western North Atlantic. Mem. Sears Found. Mar. Res., 1: 900-916.
  13. Tabeta, O. and N. Mochioka. 1988. Family Muraenidae. In: Okiyama M. (ed.), An Atlas of the Early Stage Fishes in Japan. Tokai Univ. Press, Tokyo, pp. 37-42.
  14. Tamura, K., J. Dudley, M. Nei and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24: 1596-1599. https://doi.org/10.1093/molbev/msm092
  15. Tawa, A. and N. Mochioka. 2009. Identification of aquarium-raised muraenid leptocephali, Gymnothorax minor. Ichthyol. Res., 56: 340-345. https://doi.org/10.1007/s10228-009-0104-3
  16. Tawa, A., H. Kishimoto, T. Yoshimura and N. Mochioka. 2012a. Larval identification following metamorphosis in the slender brown moray Strophidon ui from the western North Pacific. Ichthyol. Res., 59: 8-13. https://doi.org/10.1007/s10228-011-0240-4
  17. Tawa, A., M. Kobayakawa, T. Yoshimura and N. Mochioka. 2012b. Identification of leptocephalus larvae of the tiger moray Scuticaria tigrina (Anguilliformes; Muraenidae) based on morphometric and genetic evidence. Ichthyol. Res., 59: 378-383 https://doi.org/10.1007/s10228-012-0292-0
  18. Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucl. Acids Res., 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  19. Wang, C.H., C.H. Kuo, H.K. Mok and S.C. Lee. 2002. Molecular phylogeny of elopomorph fishes inferred from mitochondrial 12S ribosomal RNA sequences. Zool. scr., 32: 231-241.