Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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A Parentage Test using Indel, Microsatellite Markers and Genotypes of MC1R in the Jeju Black Cattle Population

제주 흑우 집단에서 Indel, Microsatellite 마커와 MC1R 유전자형을 이용한 친자 확인

  • Han, Sang Hyun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, In-Cheol (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, Won-Mo (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Sang-Geum (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Yang, Sung-Nyun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Kang, Yong-Jun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Park, Yong-Sang (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Young-Hoon (Institute for Livestock Promotion) ;
  • Park, Se-Phil (Department of Animal Biotechnology, Jeju National University) ;
  • Kim, Eun-Young (Mirae Biotech. Co./Jeju National University Stem Cell Research Center) ;
  • Lee, Sung-Soo (National Institute of Animal Science, RDA) ;
  • Ko, Moon-Suck (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
  • 한상현 (국립축산과학원 난지축산시험장) ;
  • 조상래 (국립축산과학원 난지축산시험장) ;
  • 조인철 (국립축산과학원 난지축산시험장) ;
  • 조원모 (국립축산과학원 난지축산시험장) ;
  • 김상금 (국립축산과학원 난지축산시험장) ;
  • 양성년 (국립축산과학원 난지축산시험장) ;
  • 강용준 (국립축산과학원 난지축산시험장) ;
  • 박용상 (국립축산과학원 난지축산시험장) ;
  • 김영훈 (제주도 축산진흥원) ;
  • 박세필 (제주대학교 동물자원과학과) ;
  • 김은영 (미래생명공학연구소/제주대학교 줄기세포연구센터) ;
  • 이성수 (국립축산과학원) ;
  • 고문석 (국립축산과학원 난지축산시험장)
  • Received : 2013.07.17
  • Accepted : 2013.09.03
  • Published : 2013.09.30
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Abstract

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

Keywords

References

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