Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Development of Cryopreservation Technique of Transgenic Bovine Embryos

형질전환 소 난자의 동결보존기술 개발

  • Uhm, Sang Jun (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Yang, Jung Seok (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Lee, Su Min (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Joe, So Young (Department of Animal Science and Biotechnology, Sangji Youngseo College) ;
  • Lim, Joon Gyo (Department of Animal Sciences, Chungbuk National University) ;
  • Heo, Young-Tae (Department of Animal Sciences, Chungbuk National University) ;
  • Xu, Yong-Nan (Department of Animal Sciences, Chungbuk National University) ;
  • Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Cheong, Ki-Soo (South Branch of Gangwondo Veterinary Service LAB) ;
  • Kim, Kwang Jae (South Branch of Gangwondo Veterinary Service LAB) ;
  • Kim, Ji Tae (South Branch of Gangwondo Veterinary Service LAB) ;
  • Kim, Nam-Hyung (Department of Animal Sciences, Chungbuk National University) ;
  • Ko, Dae Hwan (Department of Animal Science and Biotechnology, Sangji Youngseo College)
  • 엄상준 (상지영서대학교 동물생명산업과) ;
  • 양정석 (상지영서대학교 동물생명산업과) ;
  • 이수민 (상지영서대학교 동물생명산업과) ;
  • 조소영 (상지영서대학교 동물생명산업과) ;
  • 임준교 (충북대학교 축산학과) ;
  • 허영태 (충북대학교 축산학과) ;
  • 허영남 (충북대학교 축산학과) ;
  • 구본철 (대구가톨릭대학교 의학과) ;
  • 정기수 (강원도가축위생시험소 남부지소) ;
  • 김광재 (강원도가축위생시험소 남부지소) ;
  • 김지태 (강원도가축위생시험소 남부지소) ;
  • 김남형 (충북대학교 축산학과) ;
  • 고대환 (상지영서대학교 동물생명산업과)
  • Received : 2013.07.05
  • Accepted : 2013.08.01
  • Published : 2013.09.30
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Abstract

The purpose of this study is to improve production efficiency of vitrified-thawed transgenic bovine embryos. Transgenic bovine embryos were produced by injection of FIV-GFP lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization. EGFP-expressing transgenic bovine blastocysts were cultured in serum-containing and serum-free medium. These blsatocysts were vitrified by pull and cut (PNC) container made with 0.25 cm plastic straw. Results indicate that total developmental rates of normal IVF embryo cultured in serum-containing and-free medium into blastocyst were not significantly different (22.3 vs 21.5%) and those of GFP-expressing transgenic bovine embryo into blastocyst showed no significant difference between serum-containing (13.9%) and-free medium (13.1%). However, developmental rate of GFP transgenic embryo was significantly (P<0.05) lower than its of normal IVF embryos. In additional study, we vitrified GFP transgenic normal bovine blastocysts using PNC vitrification method. Survival rate of vitrified-thawed GFP transgenic blastocyst (23.1%) was significantly (P<0.05) lower than its of normal blastocysts (68.9%). Although, survival rate of vitrified-thawed GFP transgenic blastocyst was lower than its of normal blastocyst, our result may suggested that PNC vitrification method is feasible to cryopreserve transgenic embryos. Our next plan will be the production of GFP express transgenic bovine derived from vitrified-thawed embryos using PNC method.

Keywords

References

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