Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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Osteogenic Response of Human Osteoblasts Derived from Mandible and Maxilla: A Preliminary Study

상, 하악골 유래 조골세포의 골형성 능: 일차 연구

  • Yang, Hoon Joo (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Brain Korea 21 2nd Program for Craniomaxillofacial Life Science) ;
  • Song, Yoon Mi (Dental Research Institute, Seoul National University) ;
  • Kim, Ri Youn (Department of Maxillofacial Cell and Developmental Biology, School of Dentistry, Seoul National University) ;
  • Oh, Ji Hye (Dental Research Institute, Seoul National University) ;
  • Cho, Tae Hyung (Dental Research Institute, Seoul National University) ;
  • Kim, In Sook (Dental Research Institute, Seoul National University) ;
  • Hwang, Soon Jung (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Brain Korea 21 2nd Program for Craniomaxillofacial Life Science)
  • 양훈주 (서울대학교 치의학전문대학원 구강악안면외과학교실, 치의학생명과학사업단 2단계 BK 21사업) ;
  • 송윤미 (서울대학교 치학연구소) ;
  • 김리연 (서울대학교 치의학전문대학원 세포 및 발생생물학교실, 치의학생명과학사업단 2단계 BK 21사업) ;
  • 오지혜 (서울대학교 치학연구소) ;
  • 조태형 (서울대학교 치학연구소) ;
  • 김인숙 (서울대학교 치학연구소) ;
  • 황순정 (서울대학교 치의학전문대학원 구강악안면외과학교실, 치의학생명과학사업단 2단계 BK 21사업)
  • Received : 2012.08.05
  • Accepted : 2013.01.15
  • Published : 2013.01.31
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Abstract

Purpose: Maxilla and mandible have different patterns of cortical and trabecular bone and different bone mineral densities, even though both are components of the jaw bone. However, cellular differences between maxilla- and mandible derived osteoblasts (OBs) have rarely been studied. We hypothesize that maxilla- and mandible-derived OBs show different responses to $17{\beta}$-estradiol (E2), which is one of the critical factors for bone formation. This study compares skeletal site-specific cell responses between maxilla- and mandible-derived human OBs to E2. Methods: Maxilla- and mandible-derived OBs derived from an identical donor were separately isolated from a total of five normal healthy subjects aged 18~44 years old, cultured with a treatment of 100 nM estrogen. The responses between maxilla- and mandible-derived OBs to E2 were evaluated and compared using cell proliferation, alkaline phosphatase (ALP) activity and gene expression of osteoprotegerin (OPG), ALP, insulin-like growth factor-1 (IGF-1), and estrogen receptor ${\alpha}$ ($ER{\alpha}$). Results: E2 did not have any distinct effects on the proliferation of both types of OBs. Mandible-derived OBs exhibited higher ALP activity than maxilla-derived OBs in the non-treated condition, which was common in all tested individuals. ALP activities of both types of OBs showed a minor increasing tendency with the treatment of E2, even though there was no statistical significance in some specimens. The gene expression of OB by E2 was diverse, depending on the individuals. There was increased expression of OPG, IGF-1, or $ER{\alpha}$ gene in the part of subjects, which was more repeated in maxilla-derived OBs. In particular, OPG or ALP induction by E appeared less frequently in mandible-derived OBs. Conclusion: Current results revealed that E2 affects maxilla- and mandible-derived OBs into facilitating the osteogenic process despite individual differences. Mandible-derived OBs are less sensitive to bone-forming gene expression by E2.

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References

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