DOI QR코드

DOI QR Code

Application of Rapid Sample Preparation Method and Monitoring for Cholesterol Content in Chicken Egg and Egg powder

  • Park, Jung-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Jeong, In-Seek (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.) ;
  • Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.) ;
  • Ahn, Jang-Hyuk (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.) ;
  • Leem, Donggil (Nutrition and Functional Food Research Team, Korea Food and Drug Administration) ;
  • Jeong, Jayoung (Nutrition and Functional Food Research Team, Korea Food and Drug Administration) ;
  • Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Received : 2013.03.24
  • Accepted : 2013.10.11
  • Published : 2013.10.31

Abstract

The aim of this study was to develop sample preparation method and evaluate the exact cholesterol content in egg and egg powder purchased from Korean markets, and to determine whether significant differences exist among various egg products, since a variety of products are available in Korean markets and there are no recent databases for cholesterol. To evaluate the cholesterol content in chicken egg sold in Korean local market, a simple method using non-heated saponification to determine cholesterol for emulsified foods was applied. The results of recovery for egg and egg powder were in a range of 92.4-105.0%, with a relative standard deviation between 1.1% and 2.8% by using gas chromatography-flame ionization detector. Therefore, the total cholesterol content in whole egg was estimated between 160.8 and 226.3 mg/egg (AV(average) $186.8{\pm}3.5$), which is similar or lower than previously reported levels. The value for cholesterol in egg powder was estimated between 2.94 and 3.49 mg/g (AV $3.23{\pm}0.15$). We suggested method that can be applicable to chicken egg and egg powder matrix as providing rapid and accurate determination of cholesterol in egg and egg powder. This information will be helpful for processed food producers for deciding food labels of cholesterol content.

Keywords

References

  1. AOAC International. (2002a) AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals. Association of Official Analytical Chemists, Gaithersburg, MD, (USA).
  2. AOAC International. (2002b) Appendix D: Guidelines for Collaborative Study Procedures To Validate Characteristics of a Method Analysis. Association of Official Analytical Chemists, Gaithersburg, MD, (USA).
  3. Beyer, R. S. and Jensen, L. S. (1989) Overestimation of the cholesterol content of eggs. J. Agric. Food Chem. 37, 917-920. https://doi.org/10.1021/jf00088a020
  4. Guardiola, F., Codony, R., Rafecas, M., and Boatella, J. (1994) Selective Gas Chromatographic Determination of Cholesterol in Eggs. J. Am. Oil Chem. Soc. 71, 867-871. https://doi.org/10.1007/BF02540464
  5. Horwitz, W. (2011a) Official Methods of Analysis of AOAC International, 18th ed, Rev 4. In: AOAC Official Method 976. 26, Cholesterol in Multicomponent Foods. Association of Official Analytical Chemists, Gaithersburg, MD (USA), 90-92.
  6. Horwitz, W. (2011b) Official Methods of Analysis of AOAC International, 18th ed, Rev 4. In: AOAC Official Method 994. 10, Cholesterol in Foods. Association of Official Analytical Chemists, Gaithersburg, MD (USA), 105-106.
  7. Hurst, W. J., Aleo, M. D., and Martin Jr, R. A. (1985) HPLC determination of the cholesterol content of egg noodles as an indicator of egg solids. J. Agric. Food Chem. 33, 820-822. https://doi.org/10.1021/jf00065a014
  8. IDF International Dairy Federation. (2001) International Standard ISO 14156IDF 172, Milk and Milk products - Extraction methods for lipids and liposoluble compounds. ISO and IDF, BRU (Belgium).
  9. IDF International Dairy Federation. (2006) International Standard ISO 18252IDF 200, Anhydrous milk fat - Determination of sterol composition by gas liquid chromatography (Routine method). ISO and IDF, BRU (Belgium).
  10. Jeong, I. S., Kwak, B. M., Ahn, J. H., Leem, D. G., Yoon, T. H., Yoon, C. Y., Jeong, J. Y., Park, J. M., and Kim, J. M. (2012). A novel sample preparation method using rapid nonheated saponification method for the determination of cholesterol in emulsified foods. J. Food Sci. 77, C1042-C1046. https://doi.org/10.1111/j.1750-3841.2012.02903.x
  11. Kang, M. H. (2009) Food values. The Korean Nutrition Society, Seoul (Korea), 578-579.
  12. Kim, J. M., Park, J. M., Yoon, T. H, Leem, D. G., Yoon, C. Y., Jeong, J. Y., Jeong, I. S., Kwak, B. M., and Ahn, J. H. (2011) Development of analysis method for cholesterol in infant formula by direct saponification. Korean J. Food Sci. An. 6, 944-951. https://doi.org/10.5851/kosfa.2011.31.6.944
  13. Korea Food Code 2012. (2012) 10.1.1.5.5) Cholesterol, 49-52.
  14. Naviglio, D., Gallo, M., Grottaglie, L., Scala, C., Ferrara, L., and Santini, A. (2012) Determination of cholesterol in Italian chicken eggs. Food Chem. 132, 701-708. https://doi.org/10.1016/j.foodchem.2011.11.002
  15. Sweeney, J. P. and WeBirauch, J. L. (1976) Summary of available data for cholesterol in foods and methods for its determination. CRC Crit Rev. Food Sci. Nutr. 8, 131-159. https://doi.org/10.1080/10408397609527220
  16. Taber, D. F., Li, R., and Anson, C. M. (2011) Isolation of Cholesterol from an Egg Yolk. J. Chem. Eeduc. 88, 1580-1581. https://doi.org/10.1021/ed101188q
  17. United States Department of Agriculture. (2011) USDA National Nutrient Database for Standard Reference, Release 24. USDA Agricultural Research Service, Beltsville, MD, (USA).

Cited by

  1. Detection for Non-Milk Fat in Dairy Product by Gas Chromatography vol.36, pp.2, 2016, https://doi.org/10.5851/kosfa.2016.36.2.206
  2. Determination of the Authenticity of Dairy Products on the Basis of Fatty Acids and Triacylglycerols Content using GC Analysis vol.34, pp.3, 2014, https://doi.org/10.5851/kosfa.2014.34.3.316
  3. Monitoring of Used Frying Oils and Frying Times for Frying Chicken Nuggets Using Peroxide Value and Acid Value vol.36, pp.5, 2013, https://doi.org/10.5851/kosfa.2016.36.5.612
  4. Analytical methods for cholesterol quantification vol.27, pp.2, 2013, https://doi.org/10.1016/j.jfda.2018.09.001