Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization of Conditions for the Production and Properties of Alginate-degrading Crude Enzyme from Shewanella oneidensis PKA 1008

Shewanella oneidensis PKA 1008의 알긴산 분해 조효소 생산 최적 조건과 조효소의 특성

  • Sunwoo, Chan (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University) ;
  • Kim, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Jung, Seul-A (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Kim, Hyun-Jee (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Jeong, Da-Hyun (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Jung, Hee-Ye (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Kang, Bo-Kyeong (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Bark, Si-Woo (Department of Food Science & Technology/Institute, Pukyong National University) ;
  • Lim, Sung-Mee (Department of Food Science & Technology, Tongmyong University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute, Pukyong National University)
  • 선우찬 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 수산과학연구소) ;
  • 김동현 (부경대학교 식품공학과/식품연구소) ;
  • 정슬아 (부경대학교 식품공학과/식품연구소) ;
  • 김현지 (부경대학교 식품공학과/식품연구소) ;
  • 정다현 (부경대학교 식품공학과/식품연구소) ;
  • 정희예 (부경대학교 식품공학과/식품연구소) ;
  • 강보경 (부경대학교 식품공학과/식품연구소) ;
  • 박시우 (부경대학교 식품공학과/식품연구소) ;
  • 임성미 (동명대학교 식품공학과) ;
  • 홍용기 (부경대학교 생물공학과) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2012.12.27
  • Accepted : 2013.05.09
  • Published : 2013.09.28
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Abstract

An alginate-degrading bacterium, identified as Shewanella oneidensis PKA 1008 by 16S ribosomal RNA sequence analysis, was isolated from the green alga Ulva pertusa. Optimal conditions for the alginate-degrading ability of its crude enzyme were then determined. The optimal culture conditions for the growth of S. oneidensis PKA 1008 were pH 9, 2% NaCl, $30^{\circ}C$, and 24 hours incubation time. The crude enzyme produced by S. oneidensis PKA 1008 showed the highest alginate-degrading activity at pH 9, $30^{\circ}C$ and produced 1.001 g of reducing sugar per liter in 3.5% (w/v) sodium alginate for 1 hour.

부산 송정 연안에서 분해중인 해조류로부터 알긴산 분해 미생물을 분리 동정하고 미생물의 생육 조건 및 미생물이 생성한 조효소의 알긴산 분해 특성을 확인하였다. Ulva pertusa로부터 분리한 알긴산 분해균을 동정한 결과, Shewanella oneidensis strain로 확인되었으며, S. oneidensis PKA 1008 명명하였다. S. oneidensis PKA 1008의 최적 생육 조건을 확인한 결과, pH 9, 2% NaCl, $30^{\circ}C$ 및 배양 24시간인 것으로 확인되었다. 또한 S. oneidensis PKA 1008 유래 알긴산 분해 조효소는 pH 9, $30^{\circ}C$에서 분해 활성이 최대이며, 3.5% 알긴산(working concentration)에서 1시간 반응 시 1.001 g/l의 환원당을 생성하는 것으로 확인되었다.

Keywords

References

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