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통초.희렴 추출물의 항산화.항비만 활성 및 혼합물의 항비만 시너지 효과

Anti-Oxidative and Anti-Obesity Activities of Tetrapanax papyriferus and Siegesbeckia pubescens Extracts and their Synergistic Anti-Obesity Effects

  • 박정애 (동의대학교 블루바이오소재개발 및 실용화 지원센터) ;
  • 진경숙 (동의대학교 블루바이오소재개발 및 실용화 지원센터) ;
  • 이지영 (동의대학교 블루바이오소재개발 및 실용화 지원센터) ;
  • 권현주 (동의대학교 블루바이오소재개발 및 실용화 지원센터) ;
  • 김병우 (동의대학교 블루바이오소재개발 및 실용화 지원센터)
  • Park, Jung Ae (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Jin, Kyong-Suk (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Lee, Ji Young (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Kwon, Hyun Ju (Blue-Bio Industry Regional Innovation Center, Dong-Eui University) ;
  • Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
  • 투고 : 2013.06.26
  • 심사 : 2013.08.09
  • 발행 : 2013.09.28

초록

본 연구에서는 통초(Tetrapanax papyriferus)와 희렴(Siegesbeckia pubescens)의 항산화 및 항비만 활성을 DPPH radical 소거능과 세포실험계를 이용하여 분석하였다. 통초와 희렴의 DPPH radical 소거능의 50% 저해능($IC_{50}$)은 각각 65.23과 47.79 ${\mu}g/ml$로 나타났다. 또한 두 시료 모두 농도 의존적으로 lipase 효소 활성을 유의적으로 억제시켰으며, 3T3-L1 preadipocyte를 이용하여 지방세포 분화 및 지방생성에 미치는 영향을 분석한 결과 통초와 희렴 모두 지방 세포 분화, 지방 축적, TG 함량 등을 독성 없이 농도의존적으로 억제함을 보였다. 이러한 통초와 희렴의 지방세포분화억제능은 핵심 작용 인자인 $C/EBP{\alpha}$, $C/EBP{\beta}$, 그리고 $PPAR{\gamma}$의 유전자 및 단백질 발현조절에서 기인함을 확인하였다. 또한 통초와 희렴 간의 항비만 시너지 효과를 분석한 결과 각 시료의 단독 처리시보다 병용 처리시 더 높은 지방세포분화억제능을 보여 두 시료간에 시너지 효과를 보유함을 확인하였다. 이러한 결과는 통초 및 희렴의 항비만 활성 및 그 작용 기전을 밝힌 것이며 추후 계속적인 연구를 통해 활성 물질의 규명이 필요할 것으로 판단된다.

In this study, the anti-oxidative and anti-obesity activities of two medicinal herb extracts, Tetrapanax papyriferus (TP) and Siegesbeckia pubescens (SP), were evaluated using DPPH radical scavenging activity assay, lipase enzyme inhibition assay, and the cell culture model system. Both methanol extracts of TP and SP showed DPPH radical scavenging activities dose-dependently, and the $IC_{50}$ of DPPH radical scavenging activities of the two medicinal herbs were 65.23 and 47.79 ${\mu}g/ml$, respectively. Furthermore, both extracts suppressed effectively lipase enzyme activity dose-dependently. Moreover, TP and SP extracts significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride (TG) contents on 3T3-L1 preadipocytes in a dose-dependent manner without cytotoxicity. Their anti-obesity effect was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$ and peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) gene and protein expressions. Furthermore, TP and SP possessed a synergistic effect on anti-obesity activity. The identification of the active compounds that confer the anti-obesity activity of TP and SP might be needed.

키워드

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