Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Performance of the xTAG$^{(R)}$ Gastrointestinal Pathogen Panel, a Multiplex Molecular Assay for Simultaneous Detection of Bacterial, Viral, and Parasitic Causes of Infectious Gastroenteritis

  • Claas, Eric C. (Department of Medical Microbiology, Leiden University Medical Center) ;
  • Burnham, Carey-Ann D. (Departments of Pathology and Immunology and Pediatrics, Washington University School of Medicine) ;
  • Mazzulli, Tony (Department of Microbiology, University Health Network/Mount Sinai Hospital, University of Toronto) ;
  • Templeton, Kate (Specialist Virology Centre, Royal Infirmary of Edinburgh) ;
  • Topin, Francois (Luminex B.V.)
  • Received : 2012.12.20
  • Accepted : 2013.03.26
  • Published : 2013.07.28
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Abstract

The xTAG$^{(R)}$ Gastrointestinal Pathogen Panel (GPP) is a multiplexed molecular test for 15 gastrointestinal pathogens. The sensitivity and specificity of this test were assessed in 901 stool specimens collected from pediatric and adult patients at four clinical sites. A combination of conventional and molecular methods was used as comparator. Sensitivity could be determined for 12 of 15 pathogens and was 94.3% overall. The specificity across all 15 targets was 98.5%. Testing for the pathogen identified was not requested by the physician in 65% of specimens. The simultaneous detection of these 15 pathogens can provide physicians with a more comprehensive assessment of the etiology of diarrheal disease.

Keywords

References

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