Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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De Novo Assembly and Comparative Analysis of the Enterococcus faecalis Genome (KACC 91532) from a Korean Neonate

  • Ham, Jun Sang (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kwak, Woori (Interdisciplinary Program in Bioinformatics, Seoul National University) ;
  • Chang, Oun Ki (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Han, Gi Sung (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Seok Geun (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Seol, Kuk Hwan (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Hyoun Wook (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kang, Geun Ho (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Beom Young (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jong Geun (Forage and Grassland Division, National Institutue of Animal Science, Rural Development Adminstration) ;
  • Kim, Kyu-Won (Interdisciplinary Program in Bioinformatics, Seoul National University) ;
  • Sung, Samsun (C&K Genomics) ;
  • Lee, Taeheon (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Seoae (C&K Genomics) ;
  • Kim, Heebal (Interdisciplinary Program in Bioinformatics, Seoul National University)
  • Received : 2013.03.13
  • Accepted : 2013.05.15
  • Published : 2013.07.28
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Abstract

Using a newly constructed de novo assembly pipeline, finished genome level assembly had been conducted for the probiotic candidate strain E. faecalis KACC 91532 isolated from a stool samples of Korean neonates. Our gene prediction identified 3,061 genes in the assembled genome of the strain. Among these, nine genes were specific only for the E. faecalis KACC 91532, compared with all of the four known reference genomes (EF62, D32, V583, OG1RF). We identified genes related to phenotypic characters and detected E. faecalis KACC 91532-specific evolutionarily accelerated genes using dN/dS analysis. From these results, we found the potential risk of KACC 91532 as a useful probiotic strain and identified some candidate genetic variations that could affect the function of enzymes.

Keywords

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