Journal of Microbiology and Biotechnology

  • 제23권6호
  • /
  • Pages.802-810
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  • 2013
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Submerged Culture Conditions for the Production of Alternative Natural Colorants by a New Isolated Penicillium purpurogenum DPUA 1275

  • Santos-Ebinuma, Valeria Carvalho (Department of Biochemical and Pharmaceutical Technology, University of Sao Paulo) ;
  • Teixeira, Maria Francisca Simas (Culture Collection DPUA/UFAM. Universidade Federal do Amazonas) ;
  • Pessoa, Adalberto Jr. (Department of Biochemical and Pharmaceutical Technology, University of Sao Paulo)
  • 투고 : 2012.11.20
  • 심사 : 2013.01.25
  • 발행 : 2013.06.28
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초록

This work aims at investigating the production of yellow, orange, and red natural colorants in a submerged culture of Penicillium purpurogenum DPUA 1275. For this purpose, different experimental conditions evaluating the effect of incubation time, type and size of inoculum, and different carbon and nitrogen sources were performed. Furthermore, the growth kinetics were obtained in the conditions of $10^8$ spores/ml and 5 mycelia agar discs during 360 h. These experiments showed that 5 mycelia agar discs and 336 h promoted the highest yellow (3.08 $UA_{400nm}$), orange (1.44 $UA_{470nm}$), and red (2.27 $UA_{490nm}$) colorants production. Moreover, sucrose and yeast extract were the most suitable carbon and nitrogen sources for natural colorants production. Thus, the present study shows a new source of natural colorants, which can be used as an alternative to others available in the market after toxicological studies.

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