Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Ginsenoside Rd from Ginsenoside Rc by ${\alpha}-{\small{L}}$-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus

  • Shin, Kyung-Chul (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Lee, Gi-Woong (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Oh, Deok-Kun (Department of Bioscience and Biotechnology, Konkuk University)
  • Received : 2012.11.06
  • Accepted : 2012.12.14
  • Published : 2013.04.28
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Abstract

Ginsenoside Rd was produced from ginsenoside Rc using a thermostable recombinant ${\alpha}-{\small{L}}$-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Rc were pH 5.5, $80^{\circ}C$, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g $l^{-1}\;h^{-1}$. The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.

Keywords

References

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