Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of a Korean Domestic Microalga, Chlamydomonas reinhardtii KNUA021, and Analysis of Its Biotechnological Potential

  • Hong, Ji Won (Department of Biology, Kyungpook National University) ;
  • Jeong, Jieun (Department of Chemistry, Kyungpook National University) ;
  • Kim, Sung Hong (Analysis Research Division, Daegu Center, Korea Basic Science Institute) ;
  • Kim, Sunghwan (Department of Chemistry, Kyungpook National University) ;
  • Yoon, Ho-Sung (Department of Biology, Kyungpook National University)
  • Received : 2011.11.29
  • Accepted : 2012.11.29
  • Published : 2013.03.28
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Abstract

A freshwater microalga, Chlamydomonas reinhardtii KNUA021, was characterized for its potential as a biochemical feedstock. Its optimal growth was observed when the culture was incubated at $25^{\circ}C$ and pH 9.4. However, the isolate was capable of survival and growth under a variety of temperatures (10-$30^{\circ}C$) and pH (pH 4.0-12.0) conditions. The total lipid content of the isolate was 21.7% of dry weight and it was found that a high-value fatty alcohol, hexadecenol ($C_{20}H_{40}O$), was autotrophically produced by strain KNUA021. In addition, a nutritionally important $C_{18:3}{\omega}3$ (${\alpha}$-linolenic acid, ALA) was also identified in this photosynthetic microorganism as one of the major fatty acids. Hence, C. reinhardtii KNUA021 appears to show promise for use in the production of microalgae-based biochemicals.

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