Reproductive and Developmental Biology

  • 제37권3호
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  • Pages.161-167
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  • 2013
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
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G-, C-Banding 및 NORs 분포 양상에 따른 한국산 단모고양이(Felis catus)의 핵형분석

The G-, C-, and NOR-Banded Karyotypes of Korean Short-hair Cat(Felis catus)

  • 조은정 (경남과학기술대학교 동물생명과학과) ;
  • 공일근 (경상대학교 축산학과) ;
  • 손시환 (경남과학기술대학교 동물생명과학과)
  • Cho, Eun Jung (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Kong, Il Keun (Department of Animal Science, Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Sohn, Sea Hwan (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
  • 투고 : 2013.09.23
  • 심사 : 2013.09.27
  • 발행 : 2013.09.30
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초록

The karyotype of Korean short-hair cat was presented using the G-, C- and NOR-banding techniques. For chromosomes preparation, the fetus skin fibroblast cells were cultured and metaphases were obtained. In results, the Korean short-hair cat had 38 chromosomes with XX or XY, which consisted of 5 pairs of metacentric chromosomes(Group A and C), 3 pairs of submetacentric chromosomes (Group B), 6 pairs of medium metacentric chromosomes except for 1 pair of medium submetacentric D2 chromosomes (Group D, E), 2 pairs of acrocentric chromosomes(Group F) and metacentric X and Y sex chromosomes. In G-banding analysis, the Korean short-hair cat exhibited a typical and identical G-banding pattern in each homologous chromosome. Total number of bands and landmarks on the G-banded chromosomes of Korean short-hair cat well correspond to those of international standardization of karyotype of domestic cat. The heterochromatins of Korean short-hair cat chromosomes distributed at terminal and/or centromere regions on almost chromosomes by C-banding analysis. In addition, the C-banding pattern showed greatly heteromorphic in some chromosomes. Using the AgNOR-staining, we found the nucleolar organizer regions(NORs) of Korean short-hair cat located at chromosomes 1p12 site in E group. The quantity and number of NORs were constant among cells.

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