Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Change of Protein Patterns in Uterine Fluid during Estrus Cycle in Pigs

돼지에서 발정 주기 동안 자궁액 내의 단백질 패턴의 변화

  • Lee, Yeon-Ju (College of Animal Life Science, Kangwon National University) ;
  • Song, Eun-Ji (College of Animal Life Science, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University)
  • 이연주 (강원대학교 동물생명과학대학) ;
  • 송은지 (강원대학교 동물생명과학대학) ;
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2013.08.28
  • Accepted : 2013.09.02
  • Published : 2013.09.30
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Abstract

An uterus is female reproductive tract organ that affected estrus cycle. During a various changes occur at uterus in estrus cycle, one of them is body fluids secretion be called uterine fluid. Therefore, the objective of this study was to investigate the changes of protein patterns using two-dimensional gel electrophoresis in uterus fluids during the follicular and luteal phases in estrus cycle of pigs. In changes of protein spots were confirmed during the follicular and luteal phases. The 136 spots were expressed in follicular phase, the 57 spots of them showed reproducibility. On the other hand, the 140 spots were expressed in luteal phase, the 73 spots of them showed reproducibility. Also, spots expressed in follicular phase were number 69 and 94 spots and spots expressed in luteal phase only were number 156, 157, 184~187, 190 and 191 spots. The spots which of higher expression levels in the luteal phase than in follicular phase were number 76 and 79 spots. In conclusion, the spots expressed in follicular and luteal phases were confirmed with difference levels and these differences are function of RNA resolving, protein synthesis and cytoskeletal architecture.

Keywords

References

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