생약학회지 (Korean Journal of Pharmacognosy)

  • 제44권3호
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  • Pages.263-268
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  • 2013
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

흰민들레 Ethyl Acetate 분획물 및 Flavonoid 화합물의 Hydrogen Peroxide와 Amyloid Beta에 의한 신경세포의 산화적 스트레스 보호 효과

The Protective Effects of the Ethyl Acetate Fraction and Flavonoids from Taraxacum coreanum against Oxidative Stress in Neuronal Cells Induced by Hydrogen Peroxide and Amyloid Beta

  • 이아영 (부산대학교 식품영양학과) ;
  • 최지명 (부산대학교 식품영양학과) ;
  • 이설림 (중앙대학교 식물시스템과학과) ;
  • 김현영 (경남과학기술대학교 식품과학부) ;
  • 이상현 (중앙대학교 식물시스템과학과) ;
  • 조은주 (부산대학교 식품영양학과)
  • Lee, Ah Young (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Sullim (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, Hyun Young (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • 투고 : 2013.08.19
  • 심사 : 2013.09.10
  • 발행 : 2013.09.30
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초록

The protective role against oxidative stress under cellular system using C6 glioma cells was studied using the ethyl acetate (EtOAc) fraction, luteolin (1), and luteolin-7-glucoside (2) of Taraxacum coreanum. C6 glioma cells showed low cell viability and high generation of reactive oxygen species (ROS) by the treatment with generator of hydrogen peroxide ($H_2O_2$) and amyloid beta ($A{\beta}_{25-35}$). However, the treatment of the EtOAc fraction attenuated the cellular oxidative stress, resulting in significant elevation of cell viability. In addition, the production of ROS formation was also decreased by the treatment of the EtOAc fraction. Compounds 1 and 2 were isolated from the EtOAc fraction, and the protective effect was evaluated. Compounds 1 and 2 led to the increase of cell viability and decrease of production of ROS against oxidative stress by $H_2O_2$ and $A{\beta}_{25-35}$. The present study indicated that the EtOAc fraction, compounds 1 and 2 from T. coreanum demonstrated protective effects against oxidative stress, suggesting the preventive role against neurodegenerative diseases.

키워드

참고문헌

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