Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effect of the Ethyl Acetate Fraction from Tulip Tree Leaf (Liriodendron tulipifera L.) on Adipogenesis in 3T3-L1 Cells

  • Nam, Hajin (Institute of Natural Medicine, Hallym University) ;
  • Jung, Harry (Department of Medical Genetics, College of Medicine, Hallym University) ;
  • Kim, Jin Kyu (Gyeonggi Institute of Science and Technology Promotion) ;
  • Suh, Jun Gyo (Institute of Natural Medicine, Hallym University)
  • Received : 2013.07.12
  • Accepted : 2013.08.16
  • Published : 2013.09.30
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Abstract

The inhibitory effects of adipogenesis on ethyl acetate (EtOAc) fraction from leaves of the Tulip tree (TT) were evaluated. Exposure to TT EtOAc fraction (25~200 ${\mu}g/mL$) for a 72 hr incubation period did not significantly change cell viability. TT EtOAc fraction, with concentrations of 100 and 200 ${\mu}g/mL$, inhibited lipid accumulation in 3T3-L1 adipocytes in a dose dependent manner in adipogenesis. The expression of $PPAR{\gamma}$ and $C/EBP{\alpha}$, essential adipogenic markers, was significantly decreased when TT EtOAc fraction was added to cells for 8 days as compared with the untreated control group. These results suggest that TT EtOAc fraction might be a potential therapeutic agent as an effective, natural alternative material for obesity treatment.

Keywords

References

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