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동질 사배체 탱자의 종자 발아 증진과 염류 과잉에 따른 엽록소 형광 반응

Improvement of Seed Germination in a Spontaneous Autotetraploid of Poncirus and Chlorophyll Fluorescence of Seedlings in Salt Stress

  • 채치원 (국립원예특작과학원 감귤시험장) ;
  • 윤수현 (국립원예특작과학원 감귤시험장) ;
  • 박재호 (국립원예특작과학원 감귤시험장) ;
  • 김민주 (국립원예특작과학원 감귤시험장) ;
  • 한승갑 (국립원예특작과학원 감귤시험장) ;
  • 강석범 (국립원예특작과학원 감귤시험장) ;
  • 고상욱 (국립원예특작과학원 감귤시험장) ;
  • 한상헌 (제주대학교 생명자원과학대학 생물산업과학부)
  • 투고 : 2013.07.25
  • 심사 : 2013.09.10
  • 발행 : 2013.09.30

초록

향상된 종자 파종법과 비료 투입에 따른 신속한 발아와 생육 촉진된 감귤 대목 나무는 직접적인 근계의 이용면에서 더 큰 대목 특성 연구의 기회를 제공한다. 본 연구는 자연발생적인 대목 계통의 발아와 생육상에 대한 종피 제거와 비료 농도 수준에 따른 생리 반응 및 염류 스트레스에 대한 4배체의 내성 정도에 관해 평가했다. 2배체와 4배체의 종자는 박피 처리된 군과 무처리한 군을 포트에 각각 파종하여 비료 농도는 0, 2, 4, 6, 8 및 $10g{\cdot}l^{-1}$ 수준으로 시험설계는 $2{\times}6$ 요인(종피 제거${\times}$비료 농도)상의 난괴법을 따랐다. 또한 염 스트레스를 부여하기 위한 비료 농도는 0, 10, 20 및 $30g{\cdot}l^{-1}$ 수준으로 하였다. 기내에서 종피를 제거하여 치상한 처리는 무처리인 원형 그대로의 종자와는 달리 발아력이 66.7-80% 범위 내에서 향상되었다. 종피를 제거한 2배체 처리구에서는 무처리와는 달리 양액 농도가 증가할수록 수고도 증가하였다. 종피 제거는 2배체와 4배체 간에 발아를 촉진하고 높은 신장 생장을 유도하였고 영양 생장은 두 부류의 대목에서 더 농도가 높을수록 증가했다. 염 스트레스 처리 11일 후 Fv/Fm 값은 2배체에서는 0.4였지만 건전한 상태를 보이는 4배체는 0.8를 나타냈다. 종피 제거는 탱자 종자의 발아를 향상시킬 수 있으며 4배체 탱자는 2배체 탱자와 달리 염 스트레스에 대한 저항성이 있다고 판단된다.

Speed germination success and robust vegetative growth of citrus rootstock through improved sowing methods and fertilizer inputs offer the usage of root system for the citrus. The current study evaluated the influence of seed coat removal and different fertilizer concentrations on plant germination and plant growth of spontaneous rootstock siblings. Decoated and coated seeds of diploid and tetraploid plants were sown in tubes. Commercial fertilizer concentrations of 0, 2, 4, 6, 8 and $10g{\cdot}l^{-1}$ were added. The experimental layout followed a randomized block $2{\times}6$ factorial design (seed coat removal ${\times}$ fertilizer concentration) for each rootstock. Fertilizer concentrations were 0, 10, 20 and $30g{\cdot}l^{-1}$ of the fertilizer for the resistance of the strength on the salt level. The germination rate of seeds without testa sown in vitro was improved (67-80%) compared to that of nontreated seeds. The eventual tree height of the seeds without testa in the diploid group was increased due to higher fertilization compared to that in the nontreated group. The removal of seed testa promoted the seed germination of both diploid and tetraploid trifoliate orange and resulted in greater height. Their vegetative development was also increased due to the increased fertilization of the rootstock. The Fv/Fm value for the diploid plants was 0.4 and 0.8 for the tetraploid ones under salt stress after 11 days of treatment. The removal of seed testa may improve the seed germination of trifoliate orange. Tetraploid trifoliate orange appears to possess resistance to salt stress compared to the diploid variety.

키워드

참고문헌

  1. Aleza, P., Froelicher, Y., Schwarz, S., Agusti, M., Hernandez, M., Luro, J. F., Morillon, R., Navarro, L. and Ollitrault, P. 2011. Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment. Ann Bot 108, 37-50. https://doi.org/10.1093/aob/mcr099
  2. Alva, A. K. and Syvertsen, J. P. 1991. Soil and citrus tree nutrition are affected by salinized irrigation water. Proc Fla State Hort Soc 104, 135-138.
  3. Aoba, K., Sekiya, K., Iwasa, K. and Nakagawa, M. 1977. Studies on the minor metal elements in orchards. IV. Sugar-derivatives of phenolic compounds in satsuma mandarin leaves containing excess manganese resulted from manganese sulfate application. Bull Fruit Tree Res Sta A 4, 17-23.
  4. Barkley, N. A., Roose, M. L., Krueger, R. R. and Federici, C. T. 2006. Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112, 1519- 1531. https://doi.org/10.1007/s00122-006-0255-9
  5. Barrett, H. C. 1974. Colchicine-induced polyploidy in citrus. Bot Gaz 135, 29-41. https://doi.org/10.1086/336726
  6. Barrett, H. C. and Hutchison, D. J. 1978. Spontaneous tetraploidy in apomictic seedlings of citrus. Econ Bot 32, 27-45. https://doi.org/10.1007/BF02906727
  7. Bevington, K. B. and Castle, W. S. 1985. Annual root growth pattern of young citrus trees in relation to shoot growth, soil, temperature, and soil water content. J Amer Soc Hort Sci 110, 840-845.
  8. Bitters, W. P., Cole, D. A. and McCarthy, C. D. 1979. Facts about dwarf citrus trees. Citrograph 64, 54-56.
  9. Bolhár-Nordenkampf, H. R., Long, S. P., Baker, N. R., Schreiber, U. and Lechner, E. G. 1989. Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field a review of current instrumentation. Funct Ecol 3, 497-514. https://doi.org/10.2307/2389624
  10. Boman, B., Zekri, M. and Stover, E. 2005. Managing salinity in citrus production. Hort Tech 15, 108-113.
  11. Castle, W. S. and Ferguson, J. J. 1982. Current ststus of greenhouse and container production of citrus nursery trees in Florida. Proc Fla State Hort Soc 95, 42-46.
  12. Castle, W. S. and Rouse, R. E. 1990. Total mineral nutrient content of Florida citrus nursery plants. Proc Fla Sate Hort Soc 103, 42-44.
  13. Catalan, L. A. and Macchiavelli, R. E. 1991. Improving germination in Prospis flexuosa D.C. and P. alba Griseb. with hot water treatments and scarification. Seed Sci Technol 19, 253-262.
  14. Cha-um, S. and Kirdmanee, C. 2010. Effect of inland salt-affected soil on physiological and growth characters of Eucalypt tree (Eucalyptus camaldulensis Dehnh.). Emir J Food Agric 22, 466-474.
  15. Chung, B. Y., Lee, K. S., Kim, M. K., Choi, Y. H., Kim, M. K. and Cho, J. Y. 2008. Salt Accumulation and Desalinization of Rainfall Interception Culture Soils of Rubus sp. in Gochang-gun, Jeollabuk-do. Korean J Soil Sci Fert 5, 310-317.
  16. Chiang, M. H. and Park, K. W. 1994. Effects of temperature, light and mechanical treatment on the seed germination of Chrysanthemun coronarium L. J. Korean Soc Hort Sci 35, 534-539.
  17. Chilembwe, E. H. C., Castle, W. S. and Cantliffe, D. J. 1992. Grading, hydrating, and osmotically priming seed of four citrus rootstocks to increase germination rate and seedling uniformity. J Amer Soc Hort Sci 117, 368-372.
  18. Chin, H. F. 1995. Seed quality basic mechanisms and agriculture implications. pp. 209-222, The Haworth press, NY, USA.
  19. Cohen, A. 1956. Studies on the viability of citrus seeds and certain properties of their seed coats. Bull Res Council Israel Sec D 5, 200-209.
  20. Elze, D. L. 1949. Germination of citrus seeds and certain properties and their coats. Palestine J Bot 7, 69-80.
  21. Esen, A., Soost, R. K. and Geraci, G. 1978. Seed set, size, and development afer 4x ${\times}$ 2x and 4x ${\times}$ 4x crosses in Citrus. Euphytica 27, 283-294. https://doi.org/10.1007/BF00039144
  22. Fang, D. Q., Roose, M. L. Krueger, R. R. and Federici, C. T. 1997. Fingerprinting trifoliate orange germ plasm accessions with isozymes, RFLPs, and inter-simple sequence repeat markers. Theor Appl Genet 95, 211-219. https://doi.org/10.1007/s001220050550
  23. Fan, H. B. and Wang, Y. H. 2000. Effects of simulated acid rain on germination, foliar damage, chlorophyll contents and seedling growth of five hardwood species growing in China. Forest Ecol Manage 126, 321-329. https://doi.org/10.1016/S0378-1127(99)00103-6
  24. Garcia-Sanchez, F., Syvertsen, J. P., Pablo Botía, V. G. and Perez-Perez, J. G. 2007. Responses to flooding and drought stress by two citrus rootstock seedlings with different water- use efficiency. Physiol Plant 130, 532-542. https://doi.org/10.1111/j.1399-3054.2007.00925.x
  25. Gimeno, V., Syvertsen, J. P. Nieves, M., Simon, I., Martinez, V. and Garcia-Sanchez, F. 2009. Additional nitrogen fertilization affects salt tolerance of lemon trees on different rootstocks. Sci Hort 121, 298-305. https://doi.org/10.1016/j.scienta.2009.02.019
  26. Han, S. G., Lim H. C., Joa, J. H., Moon, K. H., Kang, T. W. and Song, S. J. 2008. Effects of long-term application of N, P, K fertilizers on fruit quality and yield of citrus tree (Citrus unshiu Marc.). Korean J Hort Sci Technol 26, 203-208.
  27. Han, S. G., Kim, Y. H., Moon, K. H., Lim, H. C. and U, Z. K. 2004. Salt monitoring of ground water and damage of satsuma Mandarin (Citrus unshiu Marc.) as influenced by salinity stress using sea water. Korean J Hort Sci Technol 22, 453-457.
  28. Hassanein, A. M. and Azooz, M. M. 2003. Seed propagation of Citrus reticulata. Biol Plant 47, 167-173.
  29. Heit, C. E. 1967. Propagation from seed. Part 7. Germinating 6 hardseeded groups. Am Nurseryman 125, 10-45.
  30. Karaguzel, O., Cakmakci, S., Ortacesme, V. and Aydinoglu, B. 2004. Influence of seed coat treatments on germination and early seedling growth of Lupinus varius L. Pak J Bot 36, 65-74.
  31. Kautsky, H. and Hirsch, A. 1931. Neue Versuche zur Kohlensäureassimilation. Naturwissenschaften 19, 964-964.
  32. Knight, C. A., Molinari, N. A. and Petrove, D. A. 2005. The large genome constraint hypothesis: Evolution, ecology and phenotype. Ann Bot 95, 177-190. https://doi.org/10.1093/aob/mci011
  33. Koornneef, M., Bentsink, L. and Hilhorst, H. 2002. Seed dormancy and germination. Curr Opin Plant Biol 5, 33-36. https://doi.org/10.1016/S1369-5266(01)00219-9
  34. Krezdorn, A. H. 1978. Interstocks for tree size control in citrus. Proc Fla State Hort Soc 91, 50-52.
  35. Lavorel, J. and A. L. Etienne. 1977. Primary processes of photosynthesis. pp. 203-268, Elsevier Scientific Publications Co., Amsterdam, Netherlands.
  36. Lee, E. H., Lee, J. W., Ji, J. S., Nam, Y. I., Cho, I. H. and Kwon, Y. S. 1996. Effect of substrates on growth and yield of hydroponically grown cucumber in bag culture. J Bio Fac Env 5, 15-22.
  37. Lee, E. J. and Koh, J. C. 2002. Improvement of Seed Germination in Native Iris sanguinea Donn ex Horn. Korean J Sci Technol 20, 345-351.
  38. Lee, H. Y., Park, Y. I. and Hong, Y. N. 2007. Effects of Ultraviolet-B Radiation on Photosynthesis in Tobacco (Nicotiana tabacum cv. Petit Havana SR1) Leaves. Korean J Environ Agric 26, 239-245. https://doi.org/10.5338/KJEA.2007.26.3.239
  39. Levy, Y., Lifshitz, J. De Malach, Y. and David, Y. 1999. The response of several citrus genotypes to high-salinity irrigation water. HortScience 34, 878-881.
  40. Liu, J. H., Sun, J. Y., Dai, T. B., Jiang, D., Jing, Q. and Cao, W. X. 2007. Late-growth photosynthetic characteristics and grain yield of different wheat evolutionary materials. J Plant Ecol 31, 138-144.
  41. Mademba-Sy, F., Lemerre-Desprez, Z. and Lebegin, S. 2012. Use of flying dragon trifoliate rrange as dwarfing rootstock for citrus under tropical climatic conditions. HortScience 47, 11-17.
  42. Mobayen, R. C. 1980. Germination and emergence of citrus and tomato seeds in relation to temperature. HortScience 55, 291-297.
  43. Majdi, M., Karimzadeh, G. Malboobi, M. A., Omidbaigi, R. and Mirzaghaderi, G. 2010 Induction of tetraploidy to Feverfew (Tanacetum parthenium Schulz-Bip.): morphological, physiological, cytological, and phytochemical changes HortScience 45, 16-21.
  44. Moon, D. G., Lim, H. C. Joa, J. H. Han, S. G., Hyun, H. N. and Mizutani, F. 2004. Leaf and soil chemical properties in citrus orchards occurring leaf spots disorders. J Korean Soc Hort Sci 45, 88-92.
  45. Mumford, P. M. and Grout, B. W. W. 1979. Desiccation and low temperature (-196${^{\circ}C}$) tolerance of Citrus limon seed. Seed Sci Technol 7, 407-410.
  46. Navarro, L., Roistacher, C. N. and Muurashige, T. 1975. Improvement of shoot-tip grafting in vitro for virus-free citrus. J Amer Soc Hort Sci 100, 471-479.
  47. Oh, S. E., Son, J. S., Ok, Y. S. and Joo, J. H. 2010. A modified methodology of salt removal through flooding and drainage in a plastic film house soil. Korean J Soil Sci Fert 43, 443-449.
  48. Oh, S. J., Lee, J. H., Ko, K. S. and Koh, S. C. 2012. Chlorophyll fluorescence and $CO_{2}$ fixation capacity in leaves of Camellia sinensis, Camellia japonica, and Citrus unshiu. Korean J Environ Biol 30, 98-106.
  49. Ollitrault, P., Terol, J., Chen, C., Federici, C. T., Lotfy, S., Hippolyte, I., Ollitrault, F., Bérard, A., Chauveau, A., Cuenca, J., Costantino, G., Kacar, Y., Mu, L., Garcia-Lor, A., Froelicher, Y., Aleza, P., Boland, A., Billot, C., Navarro, L., Luro, F., Roose, M. L., Gmitter, F. G., Talon, M. and Brunel, D. 2012. A reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mapping. BMC Genomics 13, 593. https://doi.org/10.1186/1471-2164-13-593
  50. Pandey, S. N. and Sinha, B. K. 1993. Plnat physiology. Pashupati print, New Dehi., India.
  51. Parida, A. K. and Das, A. B. 2005. Salt tolerance and salinity effects on plants. Ecotoxicol Saf 60, 324-349. https://doi.org/10.1016/j.ecoenv.2004.06.010
  52. Platt, R. G. and Opitz, K. W. 1974. The citrus industry, Vol. 3, pp. 1-47, Univ Calif Agr Press, Berkeley, USA.
  53. Radhamani, J., Malik, S. K., and Chandel, K. P. S. 1991. Seed coat characteristics in relation to the physiology of seed germination in Citrus and its allied genus. Seed Sci Technol 19, 611-621.
  54. Ranalli, P., Di Candilo, M. and Bagatta, M. 1997. Drought tolerance screening for potato improvement. Plant Breed 116, 290-292. https://doi.org/10.1111/j.1439-0523.1997.tb00999.x
  55. Roberts, E. H. 1973. Predicting the storage life of seeds. Seed Sci Technol 1, 499-514.
  56. Rouse, R. E. and Sherrod, J. B. 1996. Optimum temperature for citrus seed germination. Proc Fla State Hort Soc 109, 132-135.
  57. Saleh, B., Allario, T., Dambier, D., Ollitrault, P. and Morillon, R. 2008. Tetraploid citrus rootstocks are more tolerant to salt stress than diploid. C R Biol 331, 703-710. https://doi.org/10.1016/j.crvi.2008.06.007
  58. Samson, J. A. 1986. Citrus. pp.73-138, In Samson, J. A. (ed.), Tropical Fruits, 2nd edition, Longman Group, UK ltd Essex, UK.
  59. Smillie, R. M. and Nott, R. 1982. Salt tolerance in crop plants monitored by chlorophyll fluorescence in vivo. Plant Physiol 70, 1049-1054. https://doi.org/10.1104/pp.70.4.1049
  60. Soares Filho, W. S., Medrado, A. C., Cunha, M. M. A., Cunha Sobrinho, P. A. P. and Passos, O. S. 2002. Frequencia de hibridos em cruzamentos controlados de citros: cultivo de sementes versus cultivo in vitro de embrioes. Pesqui Agropecu Bras 37, 981-988. https://doi.org/10.1590/S0100-204X2002000700012
  61. Swingle, W. T. and Reece, P. C. 1967. The Citrus Industry, vol. 1. pp. 190-422, Univ Calif Press, Berkeley, USA.
  62. Syvertsen, J. P., Boman, B. and Tucker, D. P. H. 1989. Salinity in Florida citrus production. Proc Fla State Hort Soc 102, 61-64.
  63. Syvertsen, J. P. and Levy, Y. 2005. Salinity interactions with other abiotic and biotic stresses in citrus. Hort Tech 15, 100-103.
  64. Takagi, N. and Akamatsu, S. 1985. The study of minor element in late maturing citrus orchards: Soil acidfication and Mn in Miyauchi iyo orchards. J Jpn Soc Hort Sci 60, 12-13.
  65. Thompson, P. A. and Newman, P. 1979. Germination of Alstromerias. Garden 104, 75-76.
  66. Uzun, O. Gulsen, U. Seday, T. Yesiloglu, Aka-Kacar, Y. and Tuzcu, O. 2011. Investigation of genetic relationships among trifoliate oranges and their hybrid relatives based on ISSR markers. Rom Biotech Lett 16, 6430-6438.
  67. Warner, D. A. and Edwards, G. E. 1993. Effects of ploidy on photosynthesis. Photosynth Res 35, 135-147. https://doi.org/10.1007/BF00014744
  68. Weber, C. A., Moore, G. A., Deng, Z. and Gmitter, Jr G. 2003. Mapping freeze tolerance quantitative trait loci in a Citrus grandis ${\times}$ Poncirus trifoliate F1 pseudo-testcross using molecular markers. J Am Soc Hort Sci 128, 508-514.
  69. Williamson, J. G. and Castle, W. S. 1989. A survey of Florida citrus nurseries. Proc Fla State Hort Soc 102, 78-82.
  70. Wilson, J. M. and Greaves, J. A. 1993. Adaptation of food crop to temperature and water stress. pp. 389-398, AVRDC, Shanhua, Taiwan.
  71. Wullschleger, S. D., Sanderson, M. A. McLaughlin, S. B., Biradar, D. P. and Rayburn, A. L. 1996. Photosynthetic rates and ploidy levels among populations of switch grass. Crop Sci 36, 306-312. https://doi.org/10.2135/cropsci1996.0011183X003600020016x
  72. Yang, E. Y., Park, K. S., Oh, J. S., Lee, H. J. and Lee, Y. B. 2008. Effect of mineral nutrient control on nutrient uptake, growth and yield of single-node cutting rose grown in a closed hydroponic system. J Bio-Environ Cont 17, 252-260.
  73. Yeleosky, G. 1979. Accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes. Plant Physiol 64, 425-427. https://doi.org/10.1104/pp.64.3.425
  74. Yu, T. Y., Yeam, D. Y. and Kim, Y. I. 1974. Germination-promoting effect with seed coat scarifications in korean lawn grass (Zoysia japonica STEUD) seeds. J Korean Soc Hort Sci 15, 187-191.