대한본초학회지 (The Korea Journal of Herbology)

  • 제28권5호
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  • Pages.87-93
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  • 2013
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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OMC-2010 구성약재 배합추출물 투여가 Ovalbumin으로 유도한 마우스 알레르기성 기관지 천식에 미치는 영향

Effects of OMC-2010 Constituents Extract on the Ovalbumin-Induced Allergic Asthma in Mice

  • 조일주 (원광대학교 한의과대학 본초학교실) ;
  • 배기상 (원광대학교 한방체액조절연구센터) ;
  • 최선복 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실) ;
  • 서상완 (충북테크노파크 바이오센터) ;
  • 옥주안 (충북테크노파크 바이오센터) ;
  • 김민선 (한국기초과학지원연구원 질량분석연구부) ;
  • 백선종 (한국기초과학지원연구원 질량분석연구부) ;
  • 배익현 (한국기초과학지원연구원 질량분석연구부) ;
  • 김현식 (한국기초과학지원연구원 질량분석연구부)
  • Jo, Il-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Bae, Gi-Sang (Hanbang Body-fluid Research Center, Wonkwang University) ;
  • Choi, Sun-Bok (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Dept. of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Seo, Sang Wan (ChungBuk Techno Park Bio Center) ;
  • Ok, Joo An (ChungBuk Techno Park Bio Center) ;
  • Kim, Min Sun (Division of Mass Spectrometry Research, Korea Basic Science Institute) ;
  • Baek, Sun Jong (Division of Mass Spectrometry Research, Korea Basic Science Institute) ;
  • Bae, Ik Hyun (Division of Mass Spectrometry Research, Korea Basic Science Institute) ;
  • Kim, Hyun Sik (Division of Mass Spectrometry Research, Korea Basic Science Institute)
  • 투고 : 2013.08.15
  • 심사 : 2013.09.23
  • 발행 : 2013.09.30
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초록

Objectives : We recently have reported that constituents of OMC-2010 have an immuno-modulatory effects via inhibiting tumor necrosis factor (TNF)-alpha and interleukin (IL)-5. In this study, based on previous data, we investigated the effects of combinations with each OMC constituents on splenocyte cytotoxicity, cytokine productions, and ovalbumin (OVA) induced experimental allergic asthma. Methods : Mouse splenocytes were pre-treated with ethanol extract of constituents of Rehmannia glutinosa (RG), Pinellia ternata (PT), Schisandra chinensis (SC). We made 4 combinations using RG, PT, and SC (A;1:1:1, B;2:1:1, C;1:2:1, D;1:1:2). The cells were pretreated with A, B, C, or D for 1 h, then stimulated with lipopolysaccharide (LPS, $1{\mu}g/ml$) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokine productions. Then using effective combination from RG, PR and SC, we administrated the combination orally, then challenged with OVA to induce asthma. Then we analyzed the airway hyper-reactivity (AHR), lung histology and lung TNF-${\alpha}$ and IL-5 mRNA. Results : A. B. C. and D did not showed significant cytotoxicity on splenocytes. Pre-treatment of A inhibited the expression of TNF-${\alpha}$ and IL-5 significantly, but not B, C, and D. In experimental asthma, administration of A significantly inhibited the increase of AHR, lung damage, TNF-${\alpha}$ and IL-5 expression. Conclusions : Theses results could suggest that inhibitory effects of the ideal combination with RG, PT and SC (1:1:1) could be applied to treatment of asthma and study of asthma mechanisms.

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