DOI QR코드

DOI QR Code

Genetic Compositions of Broad bean wilt virus 2 Infecting Red Pepper in Korea

  • Kwak, Hae-Ryun (Crop Protection Division, National Academy of Agricultural Science) ;
  • Kim, Mi-Kyeong (Crop Protection Division, National Academy of Agricultural Science) ;
  • Nam, Moon (Crop Protection Division, National Academy of Agricultural Science) ;
  • Kim, Jeong-Soo (Crop Protection Division, National Academy of Agricultural Science) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology, Plant Genomics and Breeding Institute, Seoul National University) ;
  • Cha, Byeongjin (Department of Plant Medicine, Chungbuk National University) ;
  • Choi, Hong-Soo (Crop Protection Division, National Academy of Agricultural Science)
  • Received : 2012.12.23
  • Accepted : 2013.03.13
  • Published : 2013.09.01

Abstract

The incidence of Broad bean wilt virus 2 (BBWV2) on red pepper was investigated using the samples obtained from 24 areas of 8 provinces in Korea. Two hundred and five samples (79%) out of 260 collected samples were found to be infected with BBWV2. While the single infection rate of BBWV2 was 21.5%, the co-infection rate of BBWV2 with Cucumber mosaic virus, Pepper mottle virus, Pepper mild mottle virus and/or Potato virus Y was 78.5%. To characterize the genetic diversity of BBWV2 Korean isolates, 7 isolates were fully sequenced and analyzed. Phylogenetic analyses revealed that BBWV2 isolates could be divided largely into two groups as Group I and Group II. Based on the partial sequence analyses, 153 selected BBWV2 isolates were subgrouped into GS-I (21.6%), GS-II (3.9%) and GS-III (56.9%). BBWV2 GS-III, which was predominant in Korea, appears to be a new combination between Group I RNA-1 and Group II RNA-2. Viral disease incidence of BBWV2 on red pepper was under 2% before 2004. However, the incidence was increased abruptly to 41.3% in 2005, 58.2% in 2006 and 79% in 2007. These rapid increases might be related with the emergence of new combinations between BBWV2 groups.

Keywords

References

  1. Benner, C. P., Kuhn, C. W., Demski, J. W., Dobson, J. W., Colditz, P. and Nutter, F. W., Jr. 1985. Identification and incidence of pepper viruses in Northeastern Georgia. Plant Dis. 69:999-1001.
  2. Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J. and Watson, L. 1996. Viruses of Plants. Descriptions and Lists from the VIDE Database. CAB International, Wallingford, UK.
  3. Carvalho, C. M., Wellink, J., Ribeiro, S. G., Goldbach, R. W. and van Lent, J. W. M. 2003. The C-terminal region of themovement protein of cowpea mosaic virus is involved in binding to the large but not to the small coat protein. J. Gen. Virol. 84:2271-2277. https://doi.org/10.1099/vir.0.19101-0
  4. Chang, M. U. and Chung, J. D. 1987. Studies on viruses isolated from Lilium spp. in Korea. Plant Pathol. J. 3:223-235.
  5. Cho, J. D., Kim, J. S., Lee, S. H., Choi, G. S. and Chung, B. N. 2007. Viruses and symptoms on peppers and their infection types in Korea. Res. Plant Dis. (in Korean) 13:75-81. https://doi.org/10.5423/RPD.2007.13.2.075
  6. Choi, G. S., Kim, J. H., Lee, D. H., Kim, J. S. and Ryu, K. H. 2005. Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathol. J. 21:258-261. https://doi.org/10.5423/PPJ.2005.21.3.258
  7. Choi, H. S., Cho, J. D., Lee, K. H. and Kim, J. S. 2001. Broad bean wilt fabaviruses and their specific ultrastructures. Korean J. Electron Microscopy 31:215-222.
  8. Ferrer, R., Ferriol, I., Gueri, J., Moreno, P. and Rubio, L. 2011. Genetic variation and evolutionary analysis of Broad bean wilt virus 2. Arch. Virol. 156:1445-1450. https://doi.org/10.1007/s00705-011-0990-3
  9. Goldbach, R., Martelli, G. P. and Milne, R. G. 1995. Family Comoviridae. In: Virus taxonomy. Classification and nomenclature of viruses. Sixth report of the International Committee on Taxonomy of Viruses, ed. by F. A. Murphy, C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M. A. Mayo and M. D. Summers, pp 341-347. Springer, Wien New York, (Arch Virol [Suppl] 10).
  10. Hahm, Y. I., Kwon, M., Kim, J. S., Seo H. W. and Ahn, J. H. 1998. Serveys on disease occurrence in major horticultural crops in Kangwon Alpine areas. Plant Pathol. J. 14:668-675.
  11. Hofmann, C., Sambade, A. and Heinlein, M. 2007. Plasmodesmata and intercellular transport of viral RNA. Biochem. Soc. Transact. 35:142-145. https://doi.org/10.1042/BST0350142
  12. Ikegami, M., Kawashima, H., Natsuaki, T. and Sugimura, N. 1998. Complete nucleotide sequence of the genome organization of RNA2 of Patchouli mild mosaic virus, a new fabavirus. Arch. Virol. 143:2431-2434. https://doi.org/10.1007/s007050050472
  13. Ikegami, M., Onobori, Y., Sugimura, N. and Natsuaki, T. 2001. Complete nucleotide sequence and the genome organization of Patchouli mild mosaic virus RNA1. Intervirology 44:355-358. https://doi.org/10.1159/000050071
  14. Kim, J. H., Choi, G. S. and Choi, J. K. 2002. Characterization of Cucumber mosaic virus subgroup II isolated from paprika (Capsicum annuum var. grossum) in Korea. Plant Pathol. J. 18:6-11. https://doi.org/10.5423/PPJ.2002.18.1.006
  15. Kim, J. S., Kim, S. K., Choi, G. S. and Lee, M. W. 1990. Virus disease incidence and symptom appearance in red pepper. Korean J. Plant Pathol. 6:125-132.
  16. Kobayashi, Y. O., Mikoshiba, Y., Honda, Y. and Omura, T. 2004. Detection of Broad bean wilt virus 1 and Broad bean wilt virus 2 in Japan. Ann. Rept. Kanto Plant Prot. Soc. 51:43-48.
  17. Kobayashi, Y. O., Nakano, M., Kashiwazaki, S., Naito, T., Mikoshiba, Y., Shiota, A., Kameya-Iwaki, M. and Honda, Y. 1999. Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species. Arch. Virol. 144:1429-1438. https://doi.org/10.1007/s007050050599
  18. Kobayashi, Y. O., Kobayashi, A., Nakano, M., Hagiwara, K., Honda, Y. and Omura, T. 2003. Analysis of genetic relations between Broad bean wilt virus 1 and Broad bean wilt virus 2. J. Gen. Plant Pathol. 69:320-326.
  19. Kobayashi, Y. O., Nakano, M., Kashiwazaki, S., Naito, T., Mokoshiba, Y., Shiota, A., Kameya-Iwaki, M. and Honda, Y. 1999. Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species. Arch. Virol. 144:1429-1438. https://doi.org/10.1007/s007050050599
  20. Koh, L. H., Cooper, J. I. and Wong, S. M. 2001. Complete sequences and phylogenetic analyses of a Singapore isolate of Broad bean wilt fabavirus. Arch. Virol. 146:135-147. https://doi.org/10.1007/s007050170197
  21. Kondo, T., Fuji, S., Yamashita, K., Kang, D. K. and Chang, M. U. 2005. Broad bean wilt virus 2 in yams. J. Gen. Plant Pathol. 71:441-443. https://doi.org/10.1007/s10327-005-0236-x
  22. Kuroda, T., Okumura, A., Takeda, L., Miura, Y. and Suzuki, K. 2000. Nucleotide sequence and synthesis of infectious RNA from cloned cDNA of Broad bean wilt virus 2 RNA 2. Arch. Virol. 145:787-793. https://doi.org/10.1007/s007050050671
  23. Lee, U., Hong, J. S., Choi, J. K., Kim, K. C., Kim, Y. S., Curtis, I. S., Nam, H. G. and Lim, P. O. 2000. Broad bean wilt virus causes necrotic symptoms and generates defective RNAs in Capsicum annuum. Phytopathology 90:1390-1395. https://doi.org/10.1094/PHYTO.2000.90.12.1390
  24. Lee, S. H., Lee, K. W. and Chung, B. J. 1979. Investigations on the virus diseases in spinach (Spinacia oleracea L.) 2. Identification of Broad bean wilt virus occuring spinach. Korean J. Plant Port. 18:11-14.
  25. Lisa, V. and Boccardo, G. 1996. Fabaviruses: broad bean wilt and allied viruses. In: The plant viruses, vol. 5: Polyhedral virions and bipartite RNA genomes, ed. by B. D. Harrison and A. F. Murant, pp. 229-250. Plenum Press, New York.
  26. Liu, C., Meng, C., Xie, L., Hong, J. and Zhoua, X. 2009. Cell-tocell trafficking, subcellular distribution, and binding to coat protein of Broad bean wilt virus 2 VP37 protein. Virus Res. 143:86-93. https://doi.org/10.1016/j.virusres.2009.03.009
  27. Nakamura, S., Iwai, T. and Honkura, R. 1998. Complete nucleotide sequence and genome organization of Broad bean wilt virus 2. Ann. Phytopathol. Soc. Jpn. 64:565-568. https://doi.org/10.3186/jjphytopath.64.565
  28. Palukaitis P. and Garcia-Arenal, F. 2003. Cucumoviruses. Adv Virus Res. 62:241-323. https://doi.org/10.1016/S0065-3527(03)62005-1
  29. Park, I. S., Kim, K. W., Kyun, H. J. and Chang, M. U. 1998. The viruses in gladiolus hybridus cultivated in Korea 2. Broad bean wilt virus, Cucumber mosaic virus and Tobacco rattle virus. Plant Pathol. J. 14:83-91.
  30. Qi, Y., Zhou, X. and Li, D. 2000a. Complete nucleotide sequence and infectious cDNA clone of the RNA1 of a Chinese isolate of Broad bean wilt virus 2. Virus Genes 20:201-207. https://doi.org/10.1023/A:1008132310111
  31. Qi, Y., Zhou, X., Xue, C. and Li, D. 2000b. Nucleotide sequence of RNA2 and polyprotein processing sites of a Chinese isolate of Broad bean wilt virus 2. Prog. Nat. Sci. 10:680-686.
  32. Roh, S. H. and Chang, M. U. 1998. Three viruses isolated from Gentiana spp. in Korea. Plant Pathol. J. 14:425-432.
  33. Sanchez-Navarro, J. A., Herranz, M. C. and Pallas, V. 2006. Cellto- cell movement of alfalfa mosaic virus can be mediated by the movement proteins of ilar-, bromo-, cucumo-, tobamo- and comoviruses and does not require virion formation. Virology 346:66-73. https://doi.org/10.1016/j.virol.2005.10.024
  34. Sui, C., Wei, J. H., Zhan, Q. Q. and Zhang, J. 2009. First report of Broad bean wilt virus 2 infecting Bupleurum chinense in China. Plant Dis. 93:844.
  35. Taylor, R. H. and Stubbs, L. L. 1972. Broad bean wilt virus. Page 81 in: Description of Plant Viruses. Commonw. Mycol. Inst./ Assoc. Appl. Biol., Warwick, U.K.
  36. Uyemoto, K. J. and Provvidenti, R. 1974. Isolation and identification of two serotypes of Broad bean wilt virus. Phytopathology 64:1547-1548. https://doi.org/10.1094/Phyto-64-1547
  37. Watterson, J. C. 1993. Development and breeding of resistance to pepper and tomato viruses. In: Resistance to Viral Diseases of Vegetables, ed. by M. M. Kyle, pp. 80-101. Timber Press, Portland OR.

Cited by

  1. First Report of Blackberry chlorotic ringspot virus in Black Raspberry (Rubus coreanus) in Korea vol.101, pp.5, 2017, https://doi.org/10.1094/PDIS-11-16-1662-PDN
  2. The Current Incidence of Viral Disease in Korean Sweet Potatoes and Development of Multiplex RT-PCR Assays for Simultaneous Detection of Eight Sweet Potato Viruses vol.30, pp.4, 2014, https://doi.org/10.5423/PPJ.OA.04.2014.0029
  3. First Report of Carnation Italian ringspot virus in Chrysanthemum zawadskii var. latilobum in Korea vol.99, pp.10, 2015, https://doi.org/10.1094/PDIS-03-15-0267-PDN
  4. First Report of Broad bean wilt virus 2 in Gynura procumbens in Korea vol.101, pp.3, 2017, https://doi.org/10.1094/PDIS-10-16-1413-PDN
  5. Molecular Characterization and Variation of the Broad bean wilt virus 2 Isolates Based on Analyses of Complete Genome Sequences vol.29, pp.4, 2013, https://doi.org/10.5423/PPJ.OA.03.2013.0036
  6. A determinant of disease symptom severity is located in RNA2 of broad bean wilt virus 2 vol.211, 2016, https://doi.org/10.1016/j.virusres.2015.09.018