Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
권호 표지
DOI QR코드

DOI QR Code

Antihyperlipidemic Activity of the Ethyl-acetate Fraction of Stereospermum Suaveolens in Streptozotocin-induced Diabetic Rats

  • Received : 2013.05.03
  • Accepted : 2013.06.24
  • Published : 2013.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Dyslipidemia in diabetes mellitus is a significant risk factor for the development of cardiovascular complications. The aim of this study was to evaluate the effect of the ethyl-acetate fraction of an ethanolic extract from Streospermum suaveolens on lipid metabolism in streptozotocin (STZ)-induced diabetic rats. Methods: Diabetes was induced by intraperitonial injection of STZ (50 mg/kg). Diabetic rats were treated with an ethyl-acetate fraction orally at doses of 200 and 400 mg/kg daily for 14 days. On the $15^{th}$ day, serum lipid profiles, such as total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), were estimated in experimental rats. The atherogenic (AI) and the coronary risk (CRI) indices were also evaluated. Results: The ethyl-acetate fraction at doses of 200 and 400 mg/kg significantly (P < 0.001) and dose-dependently reduced serum cholesterol, triglycerides and LDL, but increased HDL towards near normal levels as compared to diabetic control rats. The fraction also significantly (P < 0.001) lowered the atherogenic index (AI) and coronary risk index (CAI) in a dose-dependent manner. Conclusion: The present study demonstrated that the ethyl-acetate fraction of Stereospermum suaveolens exhibits a potent antihyperlipidemic activity in hyperglycemic rats and suggests that the plant may have therapeutic value in treating the diabetic complication of hyperlipidemia.

Keywords

References

  1. Tang WH, Maroo A, Young JB. Ischemic heart disease and congestive heart failure in diabetic patients. Med Clin North Am. 2004;88(4):1037-61. https://doi.org/10.1016/j.mcna.2004.04.008
  2. Koschinsky ML, Marcoina SM. The relationship between lipoprotein alpha and the complications of diabetes mellitus. Acta Diabet. 2003;40(2):65-76.
  3. Annapurna A, Mahalakshmi DK, Krishna KM. Antidiabetic activity of a polyherbal preparation (tincture of panchparna) in normal and diabetic rats. Indian J Exp Biol. 2001;39(5):500-2.
  4. Bhattaram VA, Ceraefe M, Kohlest C, Vest M, Deundorf H. Pharmacokinetics and bioavailabitlity of herbal medicinal products. Phytomedicine. 2002;9 Suppl 3:1-33.
  5. Kim JS, Ju JB, Choi CW, Kim SC. Hypoglycemic and antihyperlipidemic effect of four Korean medicinal plants in alloxan induced diabetic rat. American Journal of Biochemistry and Biotechnology. 2006;2 (4):154-60. https://doi.org/10.3844/ajbbsp.2006.154.160
  6. The wealth of India: Raw materials. New Delhi (India): CSIR Publication; 1976. p. 49-52.
  7. Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. Dehradun (India): International Book Distributors; 1988. p. 1848-49.
  8. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. 5th ed. New Delhi (India): National Institute of Science Communication; 1999. p. 234.
  9. Ramachandran AG, Mohandoss S. 6-O-${\beta}$-D-Glucosylscutellarein - A rare Flavone glycoside from Stereospermum suaveolens. Journal of Indian Chemical Society. 1988;65:150-89.
  10. Joshi KC, Bansal RK, Patni R. Chemical examination of the roots of Stereospermum suaveolens DC. Journal of the Indian Chemical Society. 1977;54(6):648-9.
  11. Haque MR, Rahman KM, Iskander MN, Hasan CM, Rashid MA. Stereochenols A and B, two quinones from Stereospermum chelonoides. Phytochemistry. 2006;67(24): 2663-5. https://doi.org/10.1016/j.phytochem.2006.08.014
  12. Ramachandran AG, Kotiyal JP. Stereolensin - A new flavone glucoside from Stereospermum suaveolens. Indian J Chem. 1979;18B:188-9.
  13. Sankara Subramanian S, Nagarajan S, Sulochana N. Flavonoids of the leaves of Stereospermum suaveolens. Current Science. 1972;41:102-3.
  14. Ghani A. Medicinal plants of Bangladesh. Chemical constituents and uses. Dhaka: Asiatic Society of Bangladesh; 1998. p. 390.
  15. Balasubramanian T, Lal MS, Sarkar M, Chatterjee TK. Antihyperglycemic and antioxidant activities of medicinal plant Stereospermum suaveolens in streptozotocin-induced diabetic rats. J Diet Suppl. 2009;6(3):227-51. https://doi.org/10.1080/19390210903070780
  16. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 3rd ed. Pune (India): Nirali Prakashan; 1998. p. 122-8.
  17. Trease GE, Evans WC. Pharmacognosy. 10th ed. London: Balliere Tindal; 1972. p. 107, 378.
  18. Horbone JB. Phytochemical methods. London: Chapman and Hall; 1988. p. 60-6.
  19. Ecobichon DJ. The basis of toxicology testing. 3rd ed. New York: CRC press; 1997. p. 43.
  20. Siddique O, Sun Y, Lin JC, Chien YW. Facilitated transdermal transport of insulin. J Pharm Sci. 1987;76(4):341-5. https://doi.org/10.1002/jps.2600760416
  21. Nagappa AN, Thakurdesai PA, Venkat Rao N, Singh J. Antidiabetic activity of Terminalia catappa Linn fruits. J Ethnopharmacol. 2003;88(1):45-50. https://doi.org/10.1016/S0378-8741(03)00208-3
  22. Abbott RD, Wilson PW, Kannel WB, Castelli WP. High density lipoprotein cholesterol, total cholesterol screening and myocardial infarction. The Framingham Study. Arterosclerosis. 1988;8(3)207-11. https://doi.org/10.1161/01.ATV.8.3.207
  23. Alladi S, Shanmugasundaram KR. Induction of hypercholesterolemia by supplementing soy protein with acetate-generating amino acids. Nutrition Reports International. 1989;40(5):893-990.
  24. Saravanan R, Rajendra Prasad N, Pugalandi KV. Effect of Piper betle leaf extract on alcoholic toxicity in the rat brain. J Med Food. 2003;6(3):261-5. https://doi.org/10.1089/10966200360716689
  25. Nesto RW. Beyond low-density lipoprotein: addressing the atherogenic lipid triad in type 2 diabetes mellitus and the metabolic syndrome. Am J Cardiovasc Drugs. 2005;5(6):379-87. https://doi.org/10.2165/00129784-200505060-00005
  26. Betteridge J. Lipid disorders in diabetes mellitus. In Pickup JC, Williams G, editors. Text book of diabetes. 2nd ed. London. Blackwell Science; 1997: 55.1-55.31.
  27. Murali B, Upadhyaya UM, Goyal RK. Effect of chronic treatment with Enicostemma littorale in non-insulin dependent diabetic (NIDDM) rats. J Ethnopharmacol. 2002;81(2):199-204. https://doi.org/10.1016/S0378-8741(02)00077-6
  28. Bopanna KN, Kannan J, Sushma G, Balaraman R, Rathod SP. Antidiabetic and antihyperlipidemic effect of neem seed, kernal powder on alloxan diabetic rabbits. Indian J Pharmacol. 1997;29:162-7.
  29. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic complications. Diabetes Care. 1996;19(3):257-67. https://doi.org/10.2337/diacare.19.3.257
  30. Ranganathan G, Li C, Kern PA. The translational regulation of lipoprotein lipase in diabetic rats involves the 3-untransalted region of lipoprotein lipase mRNA. J Biol Chem. 2000;275(52):40986-91. https://doi.org/10.1074/jbc.M008775200
  31. Balamurugan K, Indra N, Vanithakumari G. Effect of rifampicin on certain lipid profiles in the liver of albino rats. Indian Journal of Environment and Ecoplanning. 2009;16:25-8.
  32. Temme EH, Van Hoydonck PG, Schouten EG, Kesteloot H. Effect of a plant sterol-enriched spread on serum lipids and lipoprotein in mildly hypercholesterolaemic subjects. Acta Cardiol. 2002;57(2):111-5. https://doi.org/10.2143/AC.57.2.2005382
  33. Maruthappana V, Sakthi Shree K. Antihyperlipidemic potential of a polyherbal drug (Geriforte) on atherogenic-diet-induced hyperlipidemia: A comparison with ayurslim. Int J Chem Anal Sci. 2010;1(3):37-9.
  34. Golay A, Chen YD, Reaven GM. Effect of differences in glucose tolerance on insulin's ability to regulate carbohydrate and free fatty acid metabolism in obese individuals. J Clin Endocrinol Metab. 1986;62(6):1081-8. https://doi.org/10.1210/jcem-62-6-1081
  35. Linsel-Nitschke P, Tall AR. HDL as a target in the treatment of atherosclerotic cardiovascular disease. Nat Rev Drug Discov. 2005;4(3):193-205. https://doi.org/10.1038/nrd1658
  36. Calabresi L, Gomaraschi M, Franceschini G. Endothelial protection by high-density lipoproteins: from bench to bedside. Arterioscler Thromb Vasc Biol. 2003;23(10):1724-31. https://doi.org/10.1161/01.ATV.0000094961.74697.54
  37. Singh SK, Kesari AN, Gupta RK, Jaiswal D, Watal G. Assessment of antidiabetic potential of cynodondactylon extract in streptozotocin diabetic rats. J Ethnopharmacol. 2007;114(2):174-9. https://doi.org/10.1016/j.jep.2007.07.039
  38. Oladele SB, Ayo JO, Adaudi AO. Medicinal and physiological properties of flavonoids, coumarin derivatives and anthraquinones of plant origin. West Afr J Pharmacol Drug Res. 1995;11:134-44.